Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification

Seo, Dongmin; Fujita, Hirotaka; Sakoda, Akiyoshi

doi:10.1016/j.biortech.2011.07.034

Cited by 76 publications

(39 citation statements)

References 27 publications

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“…reported that the rate of SSF of pure cellulose (Avicel) was slightly enhanced by adding Tween 20 [17]. Studies about the mechanism of Tween additives on enzymatic hydrolysis and fermentation of lignocellulosic materials have also been proposed [18-21]. The mechanism mainly focuses on three aspects: protecting free cellulase from deactivation; decreasing cellulase protein adsorption on the substrate; and reducing unproductive binding of enzymes to lignin.…”

Section: Introductionmentioning

confidence: 99%

Tween 40 pretreatment of unwashed water-insoluble solids of reed straw and corn stover pretreated with liquid hot water to obtain high concentrations of bioethanol

Lü

Yang

et al. 2013

Biotechnol Biofuels

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BackgroundLiquid hot water (LHW) pretreatment is an effective and environmentally friendly method to produce bioethanol with lignocellulosic materials. In our previous study, high ethanol concentration and ethanol yield were obtained from water-insoluble solids (WIS) of reed straw and corn stover pretreated with LHW by using fed-batch semi-simultaneous saccharification and fermentation (S-SSF). However, high cellulase loading and the large amount of wash water possibly limit the practical application of LHW pretreatment. To decrease cellulase loading and the amount of wash water, we performed Tween 40 pretreatment before WIS was subjected to bioethanol fermentation.ResultsResults showed that the optimum conditions of Tween 40 pretreatment were as follows: Tween 40 concentration of 1.5%, WIS-to-Tween 40 ratio of 1:10 (w/v), and pretreatment time of 1 hour at ambient temperature. After Tween 40 pretreatment, cellulase loading could be greatly reduced. After Tween 40 pretreatment, the residual liquid could be recycled for utilization but slightly affected ethanol concentration and yield. The unwashed WIS could obtain a high ethanol concentration of 56.28 g/L (reed straw) and 52.26 g/L (corn stover) by Tween 40 pretreatment using fed-batch S-SSF. Ethanol yield reached a maximum of 69.1% (reed straw) and 71.1% (corn stover).ConclusionsTween 40 pretreatment was a very effective and less costly method with unwashed WIS. This pretreatment could greatly reduce cellulase loading and save wash water. Higher ethanol concentration was obtained almost without reducing ethanol yield.

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Section: Introductionmentioning

confidence: 99%

Tween 40 pretreatment of unwashed water-insoluble solids of reed straw and corn stover pretreated with liquid hot water to obtain high concentrations of bioethanol

Lü

Yang

et al. 2013

Biotechnol Biofuels

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“…Many studies have reported the effect of the surfactant Tween series on enzymatic hydrolysis and SSF and its mechanism [12,15,[18][19][20][21]. The studies showed that the addition of surfactant Tween improved enzymatic hydrolysis yields and ethanol production.…”

Section: Resultsmentioning

confidence: 99%

“…Ooshima et al reported that the rate of SSF of pure cellulose (Avicel) was slightly enhanced by adding Tween 20 [17]. Studies about the mechanism of Tween additives on enzymatic hydrolysis and fermentation of lignocellulosic materials have also been proposed [18][19][20][21]. The mechanism mainly focuses on three aspects: protecting free cellulase from deactivation; decreasing cellulase protein adsorption on the substrate; and reducing unproductive binding of enzymes to lignin.…”

Section: Introductionmentioning

confidence: 99%

- Assessment of Potential Capacity Increases at Combined Heat and Power Facilities Based on Available Corn Stover and Forest Logging Residues

2015

Fuel Production From Non-Food Biomass

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Background: Liquid hot water (LHW) pretreatment is an effective and environmentally friendly method to produce bioethanol with lignocellulosic materials. In our previous study, high ethanol concentration and ethanol yield were obtained from water-insoluble solids (WIS) of reed straw and corn stover pretreated with LHW by using fed-batch semi-simultaneous saccharification and fermentation (S-SSF). However, high cellulase loading and the large amount of wash water possibly limit the practical application of LHW pretreatment. To decrease cellulase loading and the amount of wash water, we performed Tween 40 pretreatment before WIS was subjected to bioethanol fermentation.

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“…Recently, surfactants especially non-ionic surfactants have been proven to enhance the enzymatic hydrolysis conversion from lignocellulosic biomass substrates, and can lower the enzyme requirement for a given yield effectively (Seo et al, 2011;Li et al, 2012). Therefore, we tried to improve the hydrolysis efficiency through adding certain amount of non-ionic surfactants during the hydrolysis of CCR.…”

Section: Improvement Of Hydrolysis Efficiency Of Ccr By Adding Surfacmentioning

confidence: 99%

Efficient hydrolysis of corncob residue through cellulolytic enzymes from Trichoderma strain G26 and l -lactic acid preparation with the hydrolysate

Xie

Zhao

et al. 2015

Bioresource Technology

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Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification

Cited by 76 publications

References 27 publications

Tween 40 pretreatment of unwashed water-insoluble solids of reed straw and corn stover pretreated with liquid hot water to obtain high concentrations of bioethanol

Tween 40 pretreatment of unwashed water-insoluble solids of reed straw and corn stover pretreated with liquid hot water to obtain high concentrations of bioethanol

- Assessment of Potential Capacity Increases at Combined Heat and Power Facilities Based on Available Corn Stover and Forest Logging Residues

Efficient hydrolysis of corncob residue through cellulolytic enzymes from Trichoderma strain G26 and l -lactic acid preparation with the hydrolysate

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