Structural characteristics and biological activities of a pectic-polysaccharide from okra affected by ultrasound assisted metal-free Fenton reaction

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Lotus leaves are often discarded as byproducts in the lotus industry. Polysaccharides are regarded as one of the essentially bioactive components in lotus leaves. Therefore, in order to promote the application of lotus leaves in the functional food industry, the deep eutectic solvent (DES) assisted extraction of polysaccharides from lotus leaves (LLPs) was optimized, and structural and biological properties of LLPs extracted by DES and hot water were further investigated. At the optimal extraction conditions (water content of 61.0% in DES, extraction temperature of 92 °C, liquid-solid ratio of 31.0 mL/g and extraction time of 126 min), the maximum extraction yield (5.38%) was obtained. Furthermore, LLP-D extracted by DES and LLP-W extracted by hot water possessed the same sugar residues, such as 1,4-α-D-GalAp, 1,4-α-D-GalAMep, 1,3,6-β-D-Galp, 1,4-β-D-Galp, 1,5-α-L-Araf, and 1,2-α-L-Rhap, suggesting the presence of homogalacturonan (HG), rhamnogalacturonan I and arabinogalactan in both LLP-W and LLP-D. Notably, LLP-D was much richer in HG fraction than that of LLP-W, suggesting that the DES could assist to specifically extract HG from lotus leaves. Additionally, the lower molecular weight and higher content of uronic acids were observed in LLP-D, which might contribute to its much stronger in vitro antioxidant, hypoglycemic, and immunomodulatory effects. These findings suggest that the optimized DES assisted extraction method can be a potential approach for specific extraction of acidic polysaccharides with good bioactivities from lotus leaves for applications in the functional food industry.

Section: Deep Eutectic Solvent Assisted Extraction (Dese) Of Llpsmentioning

confidence: 99%

Section: Physicochemical and Structural Characterization Of Polysaccharides From Lotus Leaves (Llps)mentioning

confidence: 99%

Section: Physicochemical and Structural Characterization Of Polysaccharides From Lotus Leaves (Llps)mentioning

confidence: 99%

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Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Feng

Huang

et al. 2021

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“…Meanwhile, the high contents of uronic acids and total polyphenolics of PPP could contribute to its strong α-glucosidase inhibitory effect [5]. In addition, the high molecular weight might be also related to the remarkable inhibitory effect of polysaccharide on α-glucosidase [32,38]. Therefore, the reduction in α-glucosidase inhibitory effect of PPP after in vitro digestion might be associated with the decrease of its molecular weight and total polyphenolics.…”

Section: Stability Of Inhibitory Activity Against α-Glucosidase Of Ppp After In Vitro Digestionmentioning

confidence: 99%

Changes in Physicochemical and Biological Properties of Polyphenolic-Protein-Polysaccharide Ternary Complexes from Hovenia dulcis after In Vitro Simulated Saliva-Gastrointestinal Digestion

et al. 2021

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The present study aimed to explore the impacts of in vitro simulated saliva-gastrointestinal digestion on physicochemical and biological properties of the polyphenolic-protein-polysaccharide ternary complex (PPP) extracted from Hovenia dulcis. The results revealed that the in vitro digestion did remarkably affect physicochemical properties of PPP, such as content of reducing sugar release, content of bound polyphenolics, and molecular weight distribution, as well as ratios of compositional monosaccharides and amino acids. In particular, the content of bound polyphenolics notably decreased from 281.93 ± 2.36 to 54.89 ± 0.42 mg GAE/g, which might be the major reason for the reduction of bioactivities of PPP after in vitro digestion. Molecular weight of PPP also remarkably reduced, which might be attributed to the destruction of glycosidic linkages and the disruption of aggregates. Moreover, although biological activities of PPP obviously decreased after in vitro digestion, the digested PPP (PPP-I) also exhibited remarkable in vitro antioxidant and antiglycation activities, as well as in vitro inhibitory effects against α-glucosidase. These findings can help to well understand the digestive behavior of PPP extracted from H. dulcis, and provide valuable and scientific supports for the development of PPP in the industrial fields of functional food and medicine.

“…In addition, long-term use of such medicines may lead to liver cell damage, hepatitis, gastrointestinal disorders, and impaired body resistance to a certain extent [7]. Growing body of literature reports that changes in diet, which involve the use of some foods of plant origin, provide an efficacious treatment to alleviate lipid metabolism disorders and its complications [8]. Thus, the demand from consumers for a healthier diet through naturally occurring, pro-health products continue to grow.…”

Section: Introductionmentioning

confidence: 99%

Effects of Tartary Buckwheat Protein on Gut Microbiome and Plasma Metabolite in Rats with High-Fat Diet

Liu

Song

Zhao

et al. 2021

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The prevalence of lipid metabolism diseases, mainly obesity, fatty liver, and hyperlipidemia, is increasing in the world. Tartary buckwheat is a kind of medicinal and edible crop, and clinical experiments have also confirmed that dietary Tartary buckwheat can effectively regulate lipid metabolism disorders. Tartary buckwheat protein (TBP), as the main active ingredient of Tartary buckwheat, has an effect of blood lipid reduction that has been widely reported. In this paper, we investigated the constituents of TBP and then evaluated the hypolipidemic effect of TBP in hyperlipidemia rats. Male Sprague–Dawley rats were fed a high-fat diet for six weeks to induce hyperlipidemia and then given TBP orally for five weeks. The effects of TBP on body weight, serum lipids, liver lipids, liver oxidative stress, pathological organization, gut microbiota, and plasma metabolites were analyzed. At the serum level, TBP supplement significantly decrease the level of LDL-C and increase the level of HDL-C. At the liver level, it can reduce the levels of TC, TG, and LDL-C. The potential mechanism of action is, on the one hand, to increase the abundance of the Lachnospiraceae and the Ruminococcaceae by modulating the gut microbiota, facilitating the productivity of short-chain fatty acids, and increasing fecal bile acid excretion and, on the other hand, may be related to the improvement of bile acid metabolism.