Structural characteristics of a highly branched and acetylated pectin from Portulaca oleracea L.

Tang, Wei; Liú, Dan; Yu, Li; Zou, Ming-Yue; Shao, Yu-Chen; Yin, Jingdong; Nie, Shaoping

doi:10.1016/j.foodhyd.2021.106659

Cited by 30 publications

(14 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HSQC spectrum (Figure ) showed the presence of resonances that are typical of pectic polysaccharides. Anomeric resonances at δ 5.27/98.4 and at 4.96/100.0 were assigned to H-1/C-1 of rhamnose (with H-6/C-6 at 1.25/16.5 ppm) and galacturonic acid, respectively, of a RG-I backbone. − The intense O -methyl resonance at δ 3.81/52.8 indicated a high degree of methyl-esterification of the uronic acid residues. A resonance at δ 5.07/70.6 was assigned to H-5/C-5 of (→ 4)-α- d -Gal p A-6-OMe-(1 →) residues, while a weak resonance at δ 4.83/71.7 was assigned to H-5/C-5 of nonesterified (→ 4)-α- d -Gal p A-(1 →) residues.…”

Section: Resultsmentioning

confidence: 97%

“…In addition, a resonance in the 13 C spectrum (not shown) at δ 170.6 was assigned to esterified α- d -Gal p A, while that for nonesterified residues (∼δ 173) was not observed. , A weak resonance at δ 2.20/20.4 indicated a low degree of O-acetylation . Intense anomeric resonances at δ 5.25/109.1 and 5.08–5.14/107.1–107.5 were assigned to terminal and (→ 5)-α- l -Ara f -(1 →) and those at δ 4.46–4.51/102.8–103.2 were assigned to (→ 6)-β- d -Gal p -(1→) and (→ 3,6)-β- d -Gal p -(1→) residues. ,,, Resonances at δ 3.72–3.86/61.2 and at δ 3.79–3.95/66.9 were assigned to H-5/C-5 of both α- l -Ara f and H-6/C-6 of β- d -Gal p residues.…”

Section: Resultsmentioning

confidence: 97%

“…56,58 A weak resonance at δ 2.20/20.4 indicated a low degree of Oacetylation. 57 Intense anomeric resonances at δ 5.25/109. 56,58 Other weaker anomeric resonances may be due to RG-II residues but could not be assigned.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Anthocyanin-Bound Pectin-Rich Fraction Extracted from New Zealand Blackcurrant (Ribes nigrum) Juice

Salleh

Goh

Sims

et al. 2021

ACS Food Sci. Technol.

View full text Add to dashboard Cite

This study was conducted because of the limited knowledge of polyphenols that are bound to pectin in blackcurrant juice. A dialyzed ethanol-precipitated fraction isolated from blackcurrant juice was found to contain carbohydrate (78% w/w), uronic acid (21% w/w), protein (4.8% w/w), anthocyanin (3.9% w/w), and calcium (2.2% w/w). The pectin-rich fraction had a pK a value of 1.67, a ζ-potential of −23.1 mV (pH 4.8), and a degree of esterification of 65.2%. Constituent sugar analysis showed mostly galacturonic acid, rhamnose, arabinose, and galactose, and NMR spectroscopic analysis showed that it was rich in rhamnogalacturonans with arabinogalactan side chains. This fraction was highly pigmented, with cyanidin 3-O-rutinoside being the major anthocyanin. Liquid chromatography showed anthocyanins retained in the fraction that failed to be extracted by methanol, suggesting the presence of bound anthocyanins. Multiangle laser light scattering data showed the presence of two fractions, ∼283 kDa present at 14.6% w/w and ∼97 kDa at 85.5% w/w. The latter also produced a higher UV 280 nm signal, signifying that proteins and/or polyphenols were present mainly in the second fraction. The existence of bound anthocyanins and proteins in pectin indicates the complexity of this biopolymer, which can help to elucidate the instability issues that can arise when blackcurrant juice is mixed with other ingredients in food systems.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Characterization of Anthocyanin-Bound Pectin-Rich Fraction Extracted from New Zealand Blackcurrant (Ribes nigrum) Juice

Salleh

Goh

Sims

et al. 2021

ACS Food Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…26 The reduced EPS was then subjected to methylation according to the reported method with minor modifications. 27 Briefly, 10 mg of dried sample was completely dissolved in 1 mL of dimethyl sulfoxide, followed by the addition of 20 mg NaOH powder under N 2 protection and stirring at room temperature for 3 h, and then methyl iodide (0.3 mL) was added dropwise upon an ice bath and stirred at room temperature for 2.5 h. The methylation reaction was terminated by the addition of ultrapure water. After extraction with dichloromethane, the methylated polysaccharide was hydrolyzed with trifluoroacetic acid (1 mL, 2 M) for 3 h at 110 °C.…”

Section: Methylation Analysismentioning

confidence: 99%

Exopolysaccharide from Lactobacillus rhamnosusZFM231 alleviates DSS‐induced colitis in mice by regulating gut microbiota

et al. 2022

View full text Add to dashboard Cite

BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis. In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated. RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment. Compared with the model group, in the EPS‐treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short‐chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti‐inflammatory factor transforning growth factor‐β significantly increased and the level of pro‐inflammatory factor tumor necrosis factor‐α significantly decreased in the colonic cells of EPS‐treated mice. CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti‐ulcerative colitis. The experimental results provide new insights into the probiotic effect of EPS. © 2022 Society of Chemical Industry.

show abstract

“…Based on the production demonstration of pultrusion-interlocking flat composite intelligent grid structure, Chunxiu et al [ 14 ] put forward several reinforcement and improvement methods and realize the manufacturing of a flat composite intelligent grid structure. Tang et al [ 15 ] propose to use evolutionary neural network to realize the global nonlinear mapping relationship between structural design parameters (input) and structural response parameters (output), instead of the finite element calculation in the optimization process. GA is taken as the optimization solver, and the response surface of neural network buckling stability is taken as the main constraint to optimize the stiffened composite grid structure.…”

Section: Related Workmentioning

confidence: 99%

Study on Structural Characteristics of Composite Smart Grille Based on Principal Component Analysis

Kong

Huazhong

Xie

2022

Computational Intelligence and Neuroscience

View full text Add to dashboard Cite

In recent years, many scholars have conducted in-depth and extensive research on the mechanical properties, preparation methods, and structural optimization of grid structural materials. In this paper, the structural characteristics of composite intelligent grid are studied by combining theoretical analysis with experiments. According to the existing conditions in the laboratory, the equilateral triangular grid structure experimental pieces were prepared. In this paper, principal component analysis combined with nearest neighbor method was used to detect the damage of composite plates. On this basis, the multiobjective robustness optimization of the structure is carried out based on artificial intelligence algorithm, which makes the structure quality and its sensitivity to uncertain parameters lower. Particle swarm optimization (PSO) is used in neural network training. The damage characteristics of different grid structures, different impact positions, and different impact energies were studied. The results show that the structural damage types, areas, and propagation characteristics are very different when the structure is impacted at different positions, which verifies that the grid structure has a good ability to limit the damage diffusion and shows that the grid structure has a good ability to resist damage.

show abstract

Structural characteristics of a highly branched and acetylated pectin from Portulaca oleracea L.

Cited by 30 publications

References 45 publications

Characterization of Anthocyanin-Bound Pectin-Rich Fraction Extracted from New Zealand Blackcurrant (Ribes nigrum) Juice

Characterization of Anthocyanin-Bound Pectin-Rich Fraction Extracted from New Zealand Blackcurrant (Ribes nigrum) Juice

Exopolysaccharide from Lactobacillus rhamnosusZFM231 alleviates DSS‐induced colitis in mice by regulating gut microbiota

Study on Structural Characteristics of Composite Smart Grille Based on Principal Component Analysis

Contact Info

Product

Resources

About