Structural characteristics of mixed pectin from ginseng berry and its anti-obesity effects by regulating the intestinal flora

Ren, Ting; Xu, Mingxiang; Zhou, Shuo; Ren, Jing; Li, Bo; Jiang, Peng; Li, Hui; Wu, Wei; Chen, Changbao; Fan, Meiling; Jiao, Lili

doi:10.1016/j.ijbiomac.2023.124687

Cited by 7 publications

(3 citation statements)

References 59 publications

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“…The glycosidic bond was positioned accordingtothe previously reported literature and methylation results;→2,4)α-Glc(1→ was assigned to the signals at δ 5.32/98.59 [36]. Chemical shifts of δ 5.32, 5.22, 5.16, 5.12, and 5.08 ppm in 1 13 Cindicate two polysaccharides all had α and β glycoside bonds.There was no signal peak at 82-88 ppm in the 13 C NMR spectrum, verifying that both polysaccharide residues were pyranose [51]. Meanwhile, the signals at 175.07 ppm (in AMPA) belong to the C-6 characteristic absorption peak of uronic acid, which verifies that AMPA is an acidic polysaccharide.…”

Section: Methylation Analysismentioning

confidence: 85%

Structure Elucidation and Immunoactivity Study of Armillaria mellea Fruiting Body Polysaccharides

Li,

Niu

et al. 2023

Separations

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Polysaccharides are the main nutritional constituents in the Armillaria mellea fruiting bodies. The structure and immunoactivity of Armillaria mellea polysaccharide are valuable to be determined for development. In the present study, two polysaccharides, including Armillaria mellea neutral polysaccharide (AMPN) and Armillaria mellea acidic polysaccharide (AMPA), were prepared and determined. AMPN and AMPA were separated and refined by the ion exchange column and gel chromatographycolumn. Analysis of AMPN and AMPA revealed molecular weights of 4.432 × 103 Da and 7.323 × 103 Da. The monosaccharide composition analysis revealed that AMPN was mainly composed of 68.3%glucose, while AMPA consisted primarily of glucose, mannose, and galactose, accompanied by 8.9% galacturonic acid and 3% fucose. Then, infrared spectra analysis, Congo red staining, methylation, and NMR spectroscopy analysis were conducted as a means to clarify the structure of AMPN and AMPA. The NMR spectra demonstrated that the two polysaccharides included both α and β-configuration glycosidic bonds. The Congo red experiment suggests that AMPN and AMPA all had obvious triple helix structures.The effects of AMPN and AMPA on immune repair ability were compared by immune deficiency mice. The activity experiment showed that AMPN and acidic polysaccharides extracted from the Armillaria fruiting body have biological immune activity. Among them, AMPA showed higher immune activity. These findings suggest that Armillaria mellea fruiting bodies may be used as a source of dietary supplements and functional products.

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Section: Methylation Analysismentioning

confidence: 85%

Structure Elucidation and Immunoactivity Study of Armillaria mellea Fruiting Body Polysaccharides

Li,

Niu

et al. 2023

Separations

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“…Ginseng pectin, a mixed pectin containing the structural domains of rhamnogalacturonan-I and homogalacturonan, improves the intestinal flora by increasing Bifidobacterium, Akkermansia, Prevotella , and Bacteroides, which increases the levels of propionic, acetic, and butyric acids and valine. These, in turn, participate in cinnamonoside-, 10-hydroxy-8-fen-2-fenuglone glucoside-, leucovorin-, 24-propylcholesterol-3-ol-, and other lipid regulation-related pathways in serum metabolite alterations, and activation of the AMP-activated protein kinase pathway, to ameliorate lipid disorders in obese rats ( Zhuang et al, 2021 ; Ren et al, 2023 ).…”

Section: Therapeutic Intervention For Amd With Tcmmentioning

confidence: 99%

Role of traditional Chinese medicine in age-related macular degeneration: exploring the gut microbiota’s influence

Yu,

Liu,

Meng

2024

Front. Pharmacol.

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The pathogenesis of age-related macular degeneration (AMD), a degenerative retinopathy, remains unclear. Administration of anti-vascular endothelial growth factor agents, antioxidants, fundus lasers, photodynamic therapy, and transpupillary warming has proven effective in alleviating symptoms; however, these interventions cannot prevent or reverse AMD. Increasing evidence suggests that AMD risk is linked to changes in the composition, abundance, and diversity of the gut microbiota (GM). Activation of multiple signaling pathways by GM metabolites, including lipopolysaccharides, oxysterols, short-chain fatty acids (SCFAs), and bile acids (BAs), influences retinal physiology. Traditional Chinese medicine (TCM), known for its multi-component and multi-target advantages, can help treat AMD by altering GM composition and regulating the levels of certain substances, such as lipopolysaccharides, reducing oxysterols, and increasing SCFA and BA contents. This review explores the correlation between GM and AMD and interventions for the two to provide new perspectives on treating AMD with TCM.

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“…Bagabaldo et al found that banana pectin ameliorated HFD-induced obesity by cholesterol and bile acid binding pathways [14]. Jiao et al reported that ginseng berry pectin improved lipid disorder in obese rats through regulating gut microbiota and activating the AMP pathway [15]. Interestingly, tomato pectin (TP) is a potential active ingredient in tomato processing residue.…”

Section: Introductionmentioning

confidence: 99%

Dietary Tomato Pectin Attenuates Hepatic Insulin Resistance and Inflammation in High-Fat-Diet Mice by Regulating the PI3K/AKT Pathway

Sun,

Wu,

Wang

et al. 2024

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Chronic metabolic disease is a serious global health issue, which is accompanied by impaired insulin resistance. Tomato pectin (TP) is a naturally soluble complex hetero-polysaccharide with various biological functions. However, the impact of TP on hepatic insulin resistance in a high-fat diet (HFD) and its potential mechanism remains largely unknown. The results revealed that TP treatment significantly decreased the liver weight, hepatic fat accumulation and hepatic injury in HFD-fed mice. TP also improved fasting blood glucose levels and glucose tolerance in HFD-fed mice. The underlying mechanisms involved in the inflammation, oxidative stress and insulin signaling in the liver were also investigated by RT-qPCR and western blot, which indicated that TP ameliorated hepatic insulin resistance by regulating the PI3K/AKT/GSK-3β pathway, increasing the expression of GLUT4, decreasing the expression of PECK and G6P as well as restoring antioxidant activities and suppressing the inflammation statues in HFD-fed mice. Our data showed that dietary TP has profound effects on hepatic insulin resistance, inflammation and oxidative stress, demonstrating that TP might be a promising therapeutic agent against insulin resistance and related chronic metabolic disease.

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Structural characteristics of mixed pectin from ginseng berry and its anti-obesity effects by regulating the intestinal flora

Cited by 7 publications

References 59 publications

Structure Elucidation and Immunoactivity Study of Armillaria mellea Fruiting Body Polysaccharides

Structure Elucidation and Immunoactivity Study of Armillaria mellea Fruiting Body Polysaccharides

Role of traditional Chinese medicine in age-related macular degeneration: exploring the gut microbiota’s influence

Dietary Tomato Pectin Attenuates Hepatic Insulin Resistance and Inflammation in High-Fat-Diet Mice by Regulating the PI3K/AKT Pathway

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