Structural characterization and anti-hypoxia activity of an exopolysaccharide isolated from fermentation broth of Lachnum sp.

Du, Zhanzhan; Zong, Shuai; Surhio, Maheen Mahwish; Xu, Ping; Liu, Yang; Ye, Ming

doi:10.1016/j.procbio.2016.06.002

Cited by 7 publications

(2 citation statements)

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“…Preparation and purification of LEP were carried out according to the previous report of our research team with little modification. 16 Carboxymethylated polysaccharide and sulfated polysaccharide were obtained according to the methods of He et al (2015) and Chen et al (2014). 17,18 Animal model 6 Male mice (18-22 g) were purchased from the Experimental Animal Center of Anhui Medical University (certificate number: no.…”

Section: Preparation Of Lep and Its Derivativesmentioning

confidence: 99%

“…14 It has been reported that polysaccharides isolated from Lachnum have preventive effects against ethanolinduced gastric ulcer, and anti-hypoxia activity. 15,16 Therefore, polysaccharides from Lachnum are considered an important class of bioactive natural products, which have been widely studied for their biochemical and medical properties. In our previous study, LEP was purified from an exopolysaccharide produced by Lachnum YM240.…”

Section: Introductionmentioning

confidence: 99%

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Hypoglycemic and hypolipidemic effects of a polysaccharide from Lachnum YM240 and its derivatives in mice, induced by a high fat diet and low dose STZ

Wang

Hou

et al. 2017

Med. Chem. Commun.

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Carboxymethylated and sulfated polysaccharides (CLEP and SLEP) were prepared from an exopolysaccharide previously obtained from YM240 (LEP) by chemical modifications. Two doses (50 mg kg and 200 mg kg b. w.) of LEP, CLEP and SLEP were orally administered to normal mice and type 2 diabetic mice (T2DM) that were induced by streptozotocin (STZ) and a high fat diet, respectively. The hypoglycemic effect was evaluated by testing the oral glucose tolerance, fasting blood glucose (FBG) levels, fasting serum insulin (FINS), glycosylated hemoglobin A1c (HbA1c), and the hypolipidemic effect was evaluated by the body, spleen, pancreas, liver and kidney weights, as well as serum triglycerides (TG), cholesterol (TC) and free fatty acids (FFA). After four weeks of administration, LEP, CLEP and SLEP showed a marked FBG fall rate of 11.2%, 44.0% and 42.5% for the high-dose and 7.43%, 38.5% and 33.1% for the low-dose, respectively, as compared to the DC group. Moreover, compared with DC mice, TC concentrations in the high-dose groups of LEP, CLEP and SLEP were significantly decreased by 29.6%, 38.7% ( < 0.05), 33.0% ( < 0.05), and TG concentrations decreased by 18.9%, 43.9% ( < 0.01), 29.0% ( < 0.05), respectively. In addition, LEP and the derivatives significantly upregulated the expression of glucokinase (GK) and adenosine monophosphate-activated protein kinase (AMPK) in the liver, AMPK and glucose transporter 4 (Glut4) in skeletal muscle and peroxysome proliferator-activated receptor (PPAR-γ) in adipose tissue, whereas downregulated the expression of glucose-6-phosphatase (G6P) in the liver; these were examined using ELISA detection kits. These results for FBG and serum lipids indicate that LEP and its derivatives possess significant hypoglycemic and hypolipidemic effects and carboxymethylation improved the hypoglycemic and hypolipidemic effects more effectively than sulfation. Therefore, the carboxymethylated and sulfated modifications were effective ways to enhance the hypoglycemic and hypolipidemic activities of polysaccharides.

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Section: Preparation Of Lep and Its Derivativesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hypoglycemic and hypolipidemic effects of a polysaccharide from Lachnum YM240 and its derivatives in mice, induced by a high fat diet and low dose STZ

Wang

Hou

et al. 2017

Med. Chem. Commun.

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Valorization of cell wall polysaccharides extracted from Liubao brick tea residues: chemical, structural, and hypoglycemic properties

Wang,

Chi,

Zhao

2024

J Sci Food Agric

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BACKGROUNDTea dregs, typically generated during the production of instant tea or tea beverages, have conventionally been regarded as waste material and routinely discarded. Nevertheless, contemporary research endeavors are concentrating on discovering efficient methods for utilizing the potential of this discarded resource.RESULTSIn this study, we employed a sequential extraction method using chemical chelating agents to extract and isolate four distinct cell wall polysaccharides, designated as CWTPS‐1 through CWTPS‐4, from the tea dregs of Liubao brick tea. A comprehensive investigation into their physicochemical, structural, and hypoglycemic properties was conducted. The analysis of chemical composition and physicochemical characteristics revealed that all four CWTPSs were characterized as acidic polysaccharides, albeit with varying chemical compositions and physicochemical attributes. Specifically, the xyloglucan fractions, CWTPS‐3 and CWTPS‐4, were found to be rich in glucose and xylose, displaying a more uniform molecular weight distribution, greater structural stability, and a more irregular surface compared to the others. Moreover, they exhibited a higher diversity of monosaccharide residues. Importantly, our research unveiled that all four CWTPSs exhibited the capacity to modulate key glucose‐regulated and antioxidant enzyme activities within HepG2 cells via the IRS‐1‐PI3K/Akt signaling pathway, thereby ameliorating cellular insulin resistance. Furthermore, our correlation analysis highlighted significant associations between monosaccharide composition and neutral sugar content with the observed hypoglycemic activity of CWTPSs.CONCLUSIONSThis study highlights the potential of utilizing tea dregs as a valuable resource, making a significant contribution to the advancement of the tea industry. Furthermore, CWTPS‐4 exhibits promising prospects for further development as a functional food ingredient or additive.This article is protected by copyright. All rights reserved.

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Bioactive polysaccharides from natural sources: A review on the antitumor and immunomodulating activities

Chakraborty

Sen²,

Mondal³

et al. 2019

Biocatalysis and Agricultural Biotechnology

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Structural characterization and anti-hypoxia activity of an exopolysaccharide isolated from fermentation broth of Lachnum sp.

Cited by 7 publications

References 41 publications

Hypoglycemic and hypolipidemic effects of a polysaccharide from Lachnum YM240 and its derivatives in mice, induced by a high fat diet and low dose STZ

Hypoglycemic and hypolipidemic effects of a polysaccharide from Lachnum YM240 and its derivatives in mice, induced by a high fat diet and low dose STZ

Valorization of cell wall polysaccharides extracted from Liubao brick tea residues: chemical, structural, and hypoglycemic properties

Bioactive polysaccharides from natural sources: A review on the antitumor and immunomodulating activities

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