Structural characterization and anti-inflammatory activity of polysaccharides from Astragalus membranaceus

Chen, Guangming; Jiang, Nan; Zheng, Junping; Hu, Haiming; Yang, Huabing; Lin, Ai Jeng; Hu, Baifei; Liu, Hongtao

doi:10.1016/j.ijbiomac.2023.124386

Cited by 53 publications

(13 citation statements)

References 61 publications

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“…The content of total carbohydrate was analyzed using the phenol-sulfuric acid method, while the protein content was determined using the Bradford method. The uronic acid content was measured using the m -hydroxydiphenyl method …”

Section: Methodsmentioning

confidence: 99%

“…The molecular weights of SALP and FALP were determined as previously described with minor modifications. , High-performance gel permeation chromatography (HPGPC) was conducted using an Ultimate3000 system (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an evaporative light scattering detector (ELSD) 6000 and a TSK gel G4000 PWxl column (7.8 mm i.d. × 30 cm, 10 μm).…”

Section: Methodsmentioning

confidence: 99%

“…The uronic acid content was measured using the m-hydroxydiphenyl method. 19 Molecular Weight Detection. The molecular weights of SALP and FALP were determined as previously described with minor modifications.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The parameters for the gradient elution were as follows: 0−60 min, 15%−25% A, and a detection wavelength of 250 nm. 19 NMR Spectroscopy Analysis. About 30 mg of both SALP and FALP was dissolved in 0.5 mL of D 2 O, and the NMR spectra were then obtained using a Bruker AVANCE III HD (Bruker, Stockholm, Sweden) operated at 600 MHz.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The chemical shifts were expressed in parts per million. 19 Surface Morphology Analysis. The freeze-dried SALP and FALP samples were mounted on a metal platform and examined using a scanning electron microscope (SEM; JSM-6510LV, JEOL, Tokyo, Japan).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Atractylodes lancea Rhizome Polysaccharide Alleviates Immunosuppression and Intestinal Mucosal Injury in Mice Treated with Cyclophosphamide

Wang,

Dong,

Xie

et al. 2023

J. Agric. Food Chem.

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Plant-derived polysaccharides, such as Atractylodes lancea rhizome polysaccharide (ALP), are good immune regulators. However, the immune regulatory mechanism of the ALP is unknown. This study aimed to evaluate the effects of ALP on the intestinal mucosal barrier and intestinal mucosal immunity of immunosuppressed mice. We also compared the activity of raw Atractylodes lancea rhizome polysaccharide (SALP) with wheat bran processed bran-fried Atractylodes lancea rhizome polysaccharide (FALP; both at 1.2 g/kg/d for mice). Our results showed that ALP effectively increased the immune organ index and blood cell count, stimulated the secretion of cytokines, and promoted the expression of occludin and zonula occludens-1 (ZO-1). ALP also promoted the expression of T cells and the secretion of sIgA. Furthermore, ALP alleviated the gut microbiota disorder in Cy-treated mice and increased the relative abundances of Lactobacillus and Faecalibaculum. ALP reversed the decrease in the level of SCFAs and promoted the expression of G protein-coupled receptor 43 (GPR43). To our knowledge, this study was the first to explore how the ALP protects the intestinal mucosal barrier and enhances intestinal mucosal immunity by alleviating the gut microbiota imbalance and metabolic disorders of SCFAs. FALP was more therapeutic than SALP, suggesting that FALP could be developed as a promising functional food component.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Atractylodes lancea Rhizome Polysaccharide Alleviates Immunosuppression and Intestinal Mucosal Injury in Mice Treated with Cyclophosphamide

Wang,

Dong,

Xie

et al. 2023

J. Agric. Food Chem.

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Structural characteristics of neutral polysaccharides purified from coix seed and its anti‐insulin resistance effects on HepG2 cells

Wu,

Liu,

Wang

et al. 2024

Food Science & Nutrition

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Coix seed is recognized as a functional medicinal food due to its valuable biological activities, with polysaccharides being the primary active compounds. In this study, an ultrasonic‐assisted enzymatic extraction technique was employed, and response surface methodology was used to optimize the yield of polysaccharides to 9.55 ± 0.26%. A novel neutral polysaccharide, CSPsN‐1, was purified with a molecular weight of 7.75 kDa. CSPsN‐1 was composed of arabinose, galactose, glucose, xylose, and mannose in molar ratios of 0.48: 7.92: 86.39: 2.42: 2.79. Its backbone composed of →4)‐α‐D‐Glcp‐(1→ and →3,4)‐α‐D‐Glcp‐(1→ units, with terminal residues of α‐D‐Glcp. In vitro experiments, CSPsN‐1 enhanced glucose consumption in insulin‐resistant HepG2 cells and upregulated GLUT4 expression by activating the PI3K/AKT signaling pathway. These findings suggest that CSPsN‐1 holds significant promise as a functional ingredient for treating insulin resistance and related metabolic disorders.

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Interplay between traditional Chinese medicine polysaccharides and gut microbiota: The elusive “polysaccharides‐bond‐bacteria‐enzyme” equation

Rong,

Shen,

Shu

2024

Phytotherapy Research

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Polysaccharides are one of the most important components of traditional Chinese medicine (TCM) and have been extensively studied for their immunomodulatory properties. The functions and effects of TCM polysaccharides are closely related to the gut microbiota, making the study of their interaction a hot topic in the field of TCM metabolism. This review follows two main inquiries: first, how the gut microbiota breaks down TCM polysaccharides to produce bioactive metabolites; and second, how TCM polysaccharides reshape the gut microbiota as a carbon source. Understanding the interaction mechanism involves a challenging equation of the structural association of TCM polysaccharides with the metabolic activities of the microbiota. This review has meticulously searched, partially organized literature spanning the past decade, that delves into the interaction mechanism between TCM polysaccharides and gut microbiota. It also gives an overview of the complex factors of the elusive “polysaccharides‐bond‐bacteria‐enzyme” equation: the complexity of polysaccharide structures, the diversity of glycosidic bond types, the communal nature of metabolizing microbiota, the enzymes involved in functional degradation by microbiota, and the hierarchical roles of polysaccharide utilization locus and gram‐positive PULs. Finally, this review aims to facilitate discussion among peers in the field of TCM microbiota and offers prospects for research in related fields, paving the way for pharmacological studies on TCM polysaccharides and gut microbiota therapeutics, and providing a reference point for further clinical research.

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Structural characterization and anti-inflammatory activity of polysaccharides from Astragalus membranaceus

Cited by 53 publications

References 61 publications

Atractylodes lancea Rhizome Polysaccharide Alleviates Immunosuppression and Intestinal Mucosal Injury in Mice Treated with Cyclophosphamide

Atractylodes lancea Rhizome Polysaccharide Alleviates Immunosuppression and Intestinal Mucosal Injury in Mice Treated with Cyclophosphamide

Structural characteristics of neutral polysaccharides purified from coix seed and its anti‐insulin resistance effects on HepG2 cells

Interplay between traditional Chinese medicine polysaccharides and gut microbiota: The elusive “polysaccharides‐bond‐bacteria‐enzyme” equation

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