Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera

Song, Shuang; Wang, Linlin; Wang, Lilong; Yu, Qi; Ai, Chunqing; Fu, Yinghuan; Yan, Chunhong; Wen, Chengrong; Zhu, Zhenjun

doi:10.1016/j.ijbiomac.2019.06.116

Cited by 27 publications

(24 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A high DS increases the density of negative charges and enables a sulfated polysaccharide to more strongly neutralize these positively charged amino acid residues (Yang et al., 2013). This is consistent with the relationship of DS and anticoagulation activity for several other polysaccharides (Liang et al., 2018; Song et al., 2019).…”

Section: Resultssupporting

confidence: 90%

Sulfation, structural analysis, and anticoagulant bioactivity of ginger polysaccharides

Wang

Tang

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

In this study, ginger polysaccharide (GP), ginger polysaccharide 1 (GP1), and ginger polysaccharide 2 (GP2) from ginger were firstly modified by sulfation. Fourier transform infrared, and nuclear magnetic resonance spectra investigation of sulfated ginger polysaccharide (SGP), sulfated ginger polysaccharide 1 (SGP1), and sulfated ginger polysaccharide 2 (SGP2) revealed that the sulfation successfully occurred with the characteristic absorption peak of polysaccharide. Congo red experiment showed that triple helical structure existed in SGP and SGP1, but random coils existed in SGP2. SGP, SGP1, and SGP2 all showed a rough and rugged surface with plenty of small pores. The blood clotting time of SGP2 or SGP at 2 mg/mL in activated partial thromboplastin time (APTT) assay was 41.42 or 38.01 s, respectively, which were approximately 1.33‐ and 1.22‐fold longer than that of the physiological saline. Compared to the saline control group, prothrombin time (PT) was increased by 1.22‐fold with the addition of GP at 2 mg/mL. However, no clotting inhibition phenomenon was observed in thrombin time test even at the concentrations that APTT and PT were obviously prolonged. It indicated that GP2, SGP2, and SGP inhibited the intrinsic pathway of coagulation, but GP inhibited both the intrinsic and extrinsic pathways of coagulation. Hence, ginger polysaccharides might be used as anticoagulants and therapeutic reagents for thrombosis.

show abstract

Section: Resultssupporting

confidence: 90%

Sulfation, structural analysis, and anticoagulant bioactivity of ginger polysaccharides

Wang

Tang

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…In previous studies, the anticoagulant activity of polysaccharides was related to their acid sugar, sulfur structures and aggregation form. 35,37,39 LAP-1 contained a higher ratio of galacturonic acid and a large number of /1)-b-D-GalAp-(4/ and /1)-b-D-GlcAp-(4/ compared with other polysaccharides and the spatial stereochemical structure of LAP-1 showed an aggregation form with branches caused by the van der Waals' forces and the hydrogen bonds which different with other plant carbohydrate polymers. The coagulation activity of LAP-1 could attributes to its low molecular weight, glycosidic linkages of galacturonic acid and aggregation form according to the results.…”

Section: Anticoagulant Activity In Vitromentioning

confidence: 96%

Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.) S. Y. Hu F

Zhang

et al. 2020

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…Compared with normal control group, GYT with concentrations ≥15 mg/ml could significantly prolong plasma APTT and TT ( p < .01), indicating that GYT had significant anticoagulant activity. However, there was no significant difference in PT values among all groups ( p > .05), suggesting that GYT from the heads of Hypomesus olidus , achieved anticoagulant effects mainly by inhibiting endogenous coagulation pathway and thrombin‐mediated fibrin formation (Song et al, ). Therefore, APTT and TT were used as anticoagulant indicators in the subsequent anticoagulant tests of polysaccharides.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, plant-and animal-derived polysaccharides have attracted wide attention because of their strong anticoagulant activities (Song et al, 2019;Wang, Zhang, et al, 2018;Wang, Li, et al, 2018). Hypomesus olidus, a freshwater fish, is mainly distributed in northeastern China, Korea, Japan, and Northern Canada (Cho et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…However, studies have shown that by-products of aquatic products contain a variety of active substances, such as protein, polysaccharide, and fatty acid (Jayathilakan, Sultana, Radhakrishna, & Bawa, 2012). They have significant anticoagulant activities (Song et al, 2019), antioxidant activities (Choksawangkarn, Phiphattananukoon, Jaresitthikunchai, & Roytrakul, 2018), and anti-inflammatory activities. Therefore, the effective utilization of by-products can not only avoid waste of resources, but also avoid environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus

Zhang

et al. 2019

Food Science & Nutrition

View full text Add to dashboard Cite

The aim of this work was to extract, isolate, and purify polysaccharides from the heads of Hypomesus olidus and to evaluate their anticoagulant activities and anticoagulant mechanisms. Response surface methodology was used to optimize the extraction conditions for ultrasonic‐assisted extraction of polysaccharides from the heads of Hypomesus olidus. The optimal extraction conditions consisted of ultrasonic power of 275 W, ultrasonic time of 50 min, and solid–liquid ratio of 5 ml/g, giving the yield of crude polysaccharides (GYT) of 7.73 ± 0.042%. Three polysaccharide fractions, GYT‐1, GYT‐2, and GYT‐3 were purified from GYT by using DEAE‐cellulose‐52 column and Sephadex G‐100 column for anticoagulant activities. The results showed that two doses (2 and 4 mg/ml) of GYT‐1 and GYT‐3 could significantly prolong (p < .01) in partial thromboplastin time (APTT) (2.19 and 2.37 times, 2.22 and 2.44 times, respectively) and thrombin time (TT) (2.39 and 2.46 times, 2.44 and 2.80 times, respectively) compared with normal control. In particular, GYT‐3 had stronger anticoagulant activity than GYT‐1, and it was composed of arabinose, fructose, glucose, and lactose with molar ratios of 0.595:1: 2.026:0.273. However, GYT‐2 had no anticoagulant activity (p > .05). In addition, anticoagulation mechanism of polysaccharides from the heads of Hypomesus olidus (GYT‐3) was evaluated. The results showed that the anticoagulant activity of GYT‐3 was based on their binding with antithrombin AT‐III. And the inhibitory effects of GYT‐3 on factor IIa and Xa were related to the concentration of AT‐III in plasma. This study may provide a new and promising anticoagulant drug.

show abstract

Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera

Cited by 27 publications

References 37 publications

Sulfation, structural analysis, and anticoagulant bioactivity of ginger polysaccharides

Sulfation, structural analysis, and anticoagulant bioactivity of ginger polysaccharides

Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.) S. Y. Hu F

Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus

Contact Info

Product

Resources

About