Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods

Chen, Haoying; Zeng, Jinsong; Wang, Bin; Cheng, Zheng; Xu, Jun; Chen, Kefu

doi:10.1016/j.carbpol.2021.118149

Cited by 138 publications

(72 citation statements)

References 83 publications

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“…The ability of polysaccharide to reduce Fe 3+ to Fe 2+ was investigated by reducing the force method. The absorbance at 700 nm was measured to describe the reduction ability of natural compounds; the greater the value of A700, the better the reduction capacity ( 59 , 60 ). As shown in Figure 4D , the reducing power of PAP1b increased slowly in concentration, V C increased rapidly, and then remains unchanged; the maximum absorbance value of V C was.952.…”

Section: Resultsmentioning

confidence: 99%

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Guo

Pan³

et al. 2022

Front. Nutr.

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A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ratio of 4.1:3.3:0.9:1.7, with an average molecular weight of 37.3 kDa. Structural characterization indicated that the backbone of PAP1b appeared to be composed mainly of → 6-β-Manp-(1 →, → 4)-α-Galp-(1 → and → 3,6)-β-Manp-(1 →) residues with some branches, and terminal of (1 →)-linked-β-Manp residues. The results of bioactivity experiments showed that PAP1b had antioxidant in vitro, esspecially on scavenging DPPH and hydroxyl radicals. Therefore, the polysaccharide from Areca catechu L. could be used as a potential antioxidant in functional food.

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Section: Resultsmentioning

confidence: 99%

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Guo

Pan³

et al. 2022

Front. Nutr.

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“…This was in accordance with the results of the monosaccharide composition and FT-IR spectroscopy analyses. In addition, the more anion groups in the PGs, the better the stability of the PG-based solutions or colloids [ 28 ]. Accordingly, the stability of PG-A, with the most anion groups, was the best, followed by PG-U, PG-H, and PG-C.…”

Section: Resultsmentioning

confidence: 99%

Comparative Characterization and Immunomodulatory Activities of Polysaccharides Extracted from the Radix of Platycodon grandiflorum with Different Extraction Methods

et al. 2022

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Platycodon grandiflorum is an edible and medicinal plant, and polysaccharides are one of its important components. To further improve the utilization rate of P. grandiflorum, we investigated the effects of four different extraction methods, including hot water, ultrasonic-assisted, acid-assisted, and alkali-assisted extractions, on the polysaccharides, which were named PG-H, PG-U, PG-C, and PG-A. The findings indicated that the extraction method had a significant impact on the yield, characteristics, and immunoregulatory activity. We observed that the yields decreased in the following order: PG-H, PG-U, PG-C, and PG-A. Galacturonic acid, glucose, galactose, and arabinose were the most prevalent monosaccharides in the four PGs. However, their proportions varied. In addition, the difference between the content of glucose and galacturonic acid was more significant. PG-U had the highest glucose content, whereas PG-C had the lowest. Galacturonic acid content was highest in PG-A, while the lowest in PG-U. The molecular weight decreased in the order of PG-U, PG-H, PG-C, and PG-A; the particle size was in the order of PG-U, PG-A, PG-H, and PG-C. Moreover, the extraction method had a great impact on immunoregulatory activity. The ability to stimulate the immune function of macrophages was as follows: PG-A > PG-C > PG-U > PG-H. The results indicated that PGs, with lower molecular weights and higher GalA content, exhibited better immune-stimulating activity. And more important the AAE method was a good way to extract polysaccharides from Platycodon grandiflorum for use as a functional product and immunological adjuvant.

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“…The ABTS radical scavenging assay was based on a published method [ 43 ], with minor modifications as follows. A 7 mmol/L ABTS working stock solution was prepared, mixed with an equal volume of 2.45 mmol/L potassium persulfate solution and placed it in the dark environment for one week at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The hydroxyl radical activity was measured based on previous reports [ 43 , 45 ]. Briefly, 1 mL of the polysaccharide solution (0.1, 0.5, 1.0, 2.0, 3.0, or 4.0 mg/mL) was mixed with 1.0 mL of an FeSO 4 solution (2 mmol/L) and 1.0 mL salicylic acid–ethanol solution (6 mmol/L).…”

Section: Methodsmentioning

confidence: 99%

Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

Tong

Wang

et al. 2022

Microb Cell Fact

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Background Polysaccharides are important active ingredients in Ophiocordyceps gracilis with many physiological functions. It can be obtained from the submerged fermentation by the anamorph (Paraisaria dubia) of Ophiocordyceps gracilis. However, it was found that the mycelial pellets of Paraisaria dubia were dense and increased in volume in the process of fermentation, and the center of the pellets was autolysis due to the lack of nutrient delivery, which extremely reduced the yield of polysaccharides. Therefore, it is necessary to excavate a fermentation strategy based on morphological regulation for Paraisaria dubia to promote polysaccharides accumulation. Results In this study, we developed a method for enhancing polysaccharides production by Paraisaria dubia using microparticle enhanced technology, talc microparticle as morphological inducer, and investigated the enhancement mechanisms by transcriptomics. The optimal size and dose of talc were found to be 2000 mesh and 15 g/L, which resulted in a high polysaccharides yield. It was found that the efficient synthesis of polysaccharides requires an appropriate mycelial morphology through morphological analysis of mycelial pellets. And, the polysaccharides synthesis was found to mainly rely on the ABC transporter-dependent pathway revealed by transcriptomics. This method was also showed excellent robustness in 5-L bioreactor, the maximum yields of intracellular polysaccharide and exopolysaccharides were 83.23 ± 1.4 and 518.50 ± 4.1 mg/L, respectively. And, the fermented polysaccharides were stable and showed excellent biological activity. Conclusions This study provides a feasible strategy for the efficient preparation of cordyceps polysaccharides via submerged fermentation with talc microparticles, which may also be applicable to similar macrofungi. Graphical Abstract

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Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods

Cited by 138 publications

References 83 publications

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Comparative Characterization and Immunomodulatory Activities of Polysaccharides Extracted from the Radix of Platycodon grandiflorum with Different Extraction Methods

Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

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