Structural characterization and comparative biomedical properties of phloroglucinol from Indian brown seaweeds

Ramachandran, Karthik; Venkatesan, Manigandan; Sheeba, Ravi; Saravanan, R; Rajesh, Pushpabai Rajaian

doi:10.1007/s10811-016-0851-2

Cited by 39 publications

(27 citation statements)

References 33 publications

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“…MALDI-TOF-MS, is a particularly suitable technique for the analysis of larger oligomers, with m/z above the upper limit of ESI-MS. In a study regarding the phlorotannin fraction of Sargassum wightii, MALDI-TOF-MS was used to confirm the presence of dimers, trimers, and hexamers of phloroglucinol [189]. This technique has been also used in combination with U/HPLC-ESI-MS providing information about the size and isomeric variation of phlorotannins.…”

Section: Phlorotannins Mass Spectrometrymentioning

confidence: 99%

“…Although has being a technique used to analyze crude extracts or fractions, in order to confirm the reliability of spectrophotometric analysis (as discussed above), NMR has been also used in the identification (or confirmation) of isolated phenolic compounds [21,27,156,176], as well as of isolated phlorotannins [32,33,43,45,46,60,62,73,101,115,116,153,154,[177][178][179]184,189,207,[215][216][217]. In fact, NMR analysis has allowed to identify different groups of isolated phlorotannins, in particular, phlorethols, fuhalols, fucols, fucophlorethols, and eckols [32,33,40,43,45,46,60,62,73,153,154,[177][178][179]184,207,[215][216][217].…”

Section: Nmr Analysis Of Isolated Phenolic Compounds From Macroalgae mentioning

confidence: 99%

“…Reversed-phase (RP) HPLC columns have been the most used in macroalgae phenolic rich extracts [32,39,62,73,157,159,169,173,180,182,[186][187][188][189]. Octadecyl ("C18" or "ODS") has been the most used stationary phase, with a particle size of 5 µm.…”

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confidence: 99%

“…More interestingly, although elution with non-pH-adjusted solvents has been successfully achieved, the addition of trifluoroacetic acid [73,189], formic acid [32,116,159,169,186], or acetic acid [62,91] has been reported in accordance to general RP-HPLC standard practices.…”

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confidence: 99%

“…)1.0 350 Dasycladus vermicularis Isolationf of a sulphated coumarin from a RP-CC fraction[21]. Sargassum wightii, S. tenerrimum and Turbinaria conoides Isolation of a phlorotannins-enriched fraction from crude extract[189]. Separation of NP-CC fraction in 5 subfractions; SF4 further purified by RP-HPLC[32,169].Superose 6 colum (300/10/13) 100 mM sodium acetate buffer (pH 6.3) 20 mM EDTA, 250 mM NaCl 0.25 210 Padina gymnospora Recovery of 2-[1 -Oxo-hexadecyl]-1,3,5-trihydroxybenzene (a phloroglucinol derivative) from RP-CC fraction [164].…”

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confidence: 99%

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The Quest for Phenolic Compounds from Macroalgae: A Review of Extraction and Identification Methodologies

et al. 2019

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The current interest of the scientific community for the exploitation of high-value compounds from macroalgae is related to the increasing knowledge of their biological activities and health benefits. Macroalgae phenolic compounds, particularly phlorotannins, have gained particular attention due to their specific bioactivities, including antioxidant, antiproliferative, or antidiabetic. Notwithstanding, the characterization of macroalgae phenolic compounds is a multi-step task, with high challenges associated with their isolation and characterization, due to the highly complex and polysaccharide-rich matrix of macroalgae. Therefore, this fraction is far from being fully explored. In fact, a critical revision of the extraction and characterization methodologies already used in the analysis of phenolic compounds from macroalgae is lacking in the literature, and it is of uttermost importance to compile validated methodologies and discourage misleading practices. The aim of this review is to discuss the state-of-the-art of phenolic compounds already identified in green, red, and brown macroalgae, reviewing their structural classification, as well as critically discussing extraction methodologies, chromatographic separation techniques, and the analytical strategies for their characterization, including information about structural identification techniques and key spectroscopic profiles. For the first time, mass spectrometry data of phlorotannins, a chemical family quite exclusive of macroalgae, is compiled and discussed.

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Section: Phlorotannins Mass Spectrometrymentioning

confidence: 99%