Structural characterization and hepatoprotective activity of a galactoglucan from Poria cocos

Cheng, Yufeng; Xie, Ying; Ge, Ji-Chun; Wang, Lei; Peng, Daiyin; Yu, Nianjun; Zhang, Yue; Jiang, Yue-hang; Luo, Jian‐Ping; Chen, Weidong

doi:10.1016/j.carbpol.2021.117979

Cited by 72 publications

(32 citation statements)

References 35 publications

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“…The characteristic absorption band at 923.26 cm −1 suggested the presence of pyranoside configurations in OFP. The peaks at 850.25 cm −1 and 838.66 cm −1 were assigned to the presence of α-type and β-type glycosidic linkages [ 12 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Fan

Yang

et al. 2021

Molecules

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The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant. O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column. The molecular weight was determined as 6.2 kDa. HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc. Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain. Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated. OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.

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Section: Resultsmentioning

confidence: 99%

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Fan

Yang

et al. 2021

Molecules

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“…After 5 days of growth and adaptation to the environment, the mice were randomly divided into PCX, PCY, PCZ, and the control (CNT) groups. Every morning, the PCX group (300 mg/kg/day, intragastric water-soluble polysaccharide administration for 15 days) ( Cheng et al, 2021 ), PCY group (300 mg/kg/day, intragastric water-insoluble polysaccharide administration for 15 days) ( Sun et al, 2019 ), PCZ group (150 mg/kg/day, daily intragastric triterpene saponin administration for 15 days) ( Chao et al, 2021 ), CNT group (with an equal amount of sterile distilled water by intraperitoneal injection, once daily for 15 consecutive days). At the end of the experiment, the male KM mice were fixed on the console, and their anuses were gently wiped with sterile cotton swabs to initiate a stress response and cause the mice to defecate.…”

Section: Methodsmentioning

confidence: 99%

“…Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos , an ancient traditional Chinese medicine, is an edible and pharmaceutical mushroom belonging to the dry sclerotium of Polypo-raceae fungi widely used in many Asian countries ( Sun, 2014 ). Named Fu-ling in Chinese, P. cocos is a highly valued tonic mushroom that grows around the old or dead roots of pine trees ( Cheng et al, 2021 ). The sclerotium of P. cocos has been used to treat edema, nephrosis, spleen deficiency, chronic gastritis, acute gastroenteric symptoms, diarrhea, indigestion, and weight loss for more than two thousand years, and it can also be consumed by healthy people to calm the nerves and strengthen the body’s immune function ( Ji et al, 2019 ; Li et al, 2019 ; Weifeng Li et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics

Deng²,

Lei

et al. 2022

Front. Pharmacol.

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Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties. Because of the mushroom’s complex composition, its pharmacological effects have not been fully clarified. Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice. These injections were made to explore the component’s effects on the mice’s gut microbiota and their metabolomics. The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01). A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites. In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota. The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.

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“…Pectin also acts as a fat substitute in spreads, ice cream and emulsified meat products ( Munoz-Almagro, Montilla, & Villamiel, 2021 ). Besides, pectin exhibits various nutritious functions such as antioxidant activity ( Ma et al, 2020 ), intestinal protection activity ( Shen et al, 2021 ), anti-inflammatory ( Xiong et al, 2021 ), immunomodulatory ( Zhu et al, 2021 ), hepatoprotective activity ( Cheng et al, 2021 ), and inhibition of tumors ( He et al, 2021 ), making it widely used in health food and pharmaceutical industries. The functions of pectin are largely influenced by its structure ( Cui et al, 2021 ), and not all isolated pectins are suitable for any potential application in food or pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Chain conformations and steady-shear viscosity properties of pectic polysaccharides from apple and tomato

Wang

Nie

et al. 2022

Food Chemistry: X

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Structural characterization and hepatoprotective activity of a galactoglucan from Poria cocos

Cited by 72 publications

References 35 publications

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics

Chain conformations and steady-shear viscosity properties of pectic polysaccharides from apple and tomato

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