Structural characterization and immunomodulatory activity of two novel polysaccharides derived from the stem of Dendrobium officinale Kimura et Migo

Tao, Shengchang; Lei, Zhouxi; Li, Yunrong; Ren, Zhiyao; Zhang, Xiaofeng; Wei, Gang; Chen, Haiming

doi:10.1016/j.jff.2019.04.013

Cited by 51 publications

(24 citation statements)

References 37 publications

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“…Another polysaccharide, DOPW-1, of Mw 389.98 kDa, was isolated from the stems of D . officinale according to a method described previously ( Tao et al, 2019 ). In brief, after hot water extraction, a D .…”

Section: Methodsmentioning

confidence: 99%

“…D . officinale polysaccharides possess anticancer ( Yu et al, 2018a ; Zhang et al, 2019 ; Zhang et al, 2020 ; Tao et al, 2021 ), immunomodulation ( Huang et al, 2016 ; Tao et al, 2019 ), antioxidant ( Luo et al, 2016 ), hypoglycemic ( Wang et al, 2018 ; Kuang et al, 2020 ) and antifatigue ( Wei et al, 2017 ) activities. However, few studies have focused on the activity of D .…”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

et al. 2021

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We investigated the antitumor effects of four fractions of Dendrobium officinale Kimura & Migo (D. officinale) polysaccharides with different molecular weights (Mw), Astragalus membranaceus polysaccharides (APS) and Lentinus edodes polysaccharides (LNT) on colorectal cancer (CRC) using a zebrafish xenograft model. Transcriptome sequencing was performed to further explore the possible antitumor mechanisms of D. officinale polysaccharides. Fractions of D. officinale polysaccharides, LNT, and APS could significantly inhibit the growth of HT-29 cells in a zebrafish xenograft model. One fraction of D. officinale polysaccharides called DOPW-1 (Mw of 389.98 kDa) exhibited the strongest tumor inhibition. Compared with the control group, RNA-seq revealed that the DOPW-1–treated experimental group had 119 differentially expressed genes (DEGs), of which 45 had upregulated expression and 74 had downregulated expression. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that the pathway “apoptosis-multiple species” was the most significantly enriched. Our data indicated that 1) fractions of D. officinale polysaccharides of Mw 389.98 kDa were most suitable against CRC; 2) DOPW-1 could be developed into a clinical agent against CRC; and 3) an apoptosis pathway is important for DOPW-1 to inhibit the proliferation of HT-29 cells.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

et al. 2021

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“…For instance, most of animal metabolomic studies have investigated effects of ginseng, Panax ginseng, and American ginseng (Table S1). Ginseng beneficially affects circulating metabolites in different metabolic pathways, such as bile acids, amino acids, lipids, and phytosphingosine (Feng, Liu, et al., 2016; Feng, Yue, et al., 2016; Li, Liu, et al., 2018; Lin, Liu, Pi, et al., 2018; Lin et al., 2016; Tao et al., 2019; Wang, Zhu, et al., 2019; Zhu et al., 2015), which may partly explain the mechanism of therapeutic effects of ginsengs on Alzheimer's disease, stress, TNBS‐induced colitis, cognitive impairment, and cisplatin nephrotoxicity. Of note, the dried leaf of Platycodon grandiflorum is known for its anti‐inflammatory and antioxidative activities.…”

Section: Metabolomics and Mechanisms Of Mfhmentioning

confidence: 99%

Advances in understanding of health‐promoting benefits of medicine and food homology using analysis of gut microbiota and metabolomics

et al. 2020

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The health-promoting benefits of medicine and food homology (MFH) are known for thousands of years in China. However, active compounds and biological mechanisms are unclear, greatly limiting clinical practice of MFH. The advent of gut microbiota analysis and metabolomics emerge as key tools to discover functional compounds, therapeutic targets, and mechanisms of benefits of MFH. Such studies hold great promise to promote and optimize functional efficacy and development of MFH-based products, for example, foods for daily dietary supplements or for special medical purposes. In this review, we summarized pharmacological effects of 109 species of MFH approved by the Health and Fitness Commission in 2015. Recent studies applying genome sequencing of gut microbiota and metabolomics to explain the activity of MFH in prevention and management of health consequences were extensively reviewed. We discussed the potentiality in future to decipher functional activities of MFH by applying metabolomics-based polypharmacokinetic strategy and multiomics technologies. The needs for personalized MFH recommendations and comprehensive databases have also been highlighted. This review emphasizes current achievements and challenges of the analysis of gut microbiota and metabolomics as a new avenue to understand MFH. K E Y W O R D S gut microbiota, medicine and food homology, metabolomics, multiomics technology Abbreviations: IBD, inflammatory bowel disease; LPS, lipopolysaccharide; MFH, medicine and food homology; SCFA, short-chain fatty acid; TCMIO, a comprehensive database of traditional Chinese medicine on immuno-oncology; WEGL, water extract of Ganoderma lucidum mycelium This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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“…Healthy effects are found in the prevention and treatments of diabetes, hypertension, obesity, protection against gastric ulcers and hypertension, and immune enhancement in animal models [3][4][5][6][7][8][9] . The widely recognised functional properties are mainly due to the various phytochemicals in the D. o cinale, which mainly include polysaccharides, phenanthrenes, bibenzyls, saccharides and glycosides, essential oils, alkaloids and polyphenols [1,10] .…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Nutritional Quality of Dendrobium Officinale Kimura Et Migo After Transplantation of Artificially Cultivated Plants From South to North China During Different Growing Years

Guo

Zhang

Yan

et al. 2021

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Background: Dendrobium officinale Kimura et Migo is a traditional functional food and medicinal plant in China. Due to low natural regeneration rates, habitat destruction, excessive collection and commercial trade, D. officinale is severely threatened, and commercial artificial-sheltered cultivation has been massively used to meet the needs of the market. Aim: To comprehensively compare the accumulation of nutritional compounds during 3-5 years of introduced, artificial-sheltered cultivation from southern to northern China.Methods: D. officinale of the same species were artificially cultivated in the southern traditional cultivation area, Anhui and the new northern cultivation area, Beijing. First, samples were collected in the third, fourth, and fifth years of growth, and nutritional quality indexes, including polysaccharides, alkaloids, flavonoids and total phenolic content, were determined. Second, an untargeted metabolomics method was used to investigate metabolic variations in D. officinale stems between Anhui and Beijing cultures in the fifth year.Results: After comparing the nutrient accumulation in different growing years, the idea harvest time was found in the third growing year in both cultivation areas. Of them, the contents of polysaccharides, flavonoids and total polyphenol were higher in cultivation in Anhui than Beijing, but the accumulation of alkaloid content was much lower in Anhui. The highest amount of polysaccharides of Dendrobium officinale was found in the three-year cultivation in Anhui, which reached 515.75 mg/g. When metabolites were analysed, a total of 272 metabolites were detected in the current study, including 27 up-regulated and 73 down-regulated metabolites in D. officinale cultivated in Beijing compared with samples from Anhui. Conclusion: D. officinale artificially and transplanted cultivated from southern to northern China showed some significant differences in the accumulation of nutrient compounds. Planting in northern China has some specific advantages, but the overall nutritional value is not as good as planting in southern China. Our study contributes to a better understanding of the nutrient profiles of D. officinale through artificial cultivation in different areas.

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Structural characterization and immunomodulatory activity of two novel polysaccharides derived from the stem of Dendrobium officinale Kimura et Migo

Cited by 51 publications

References 37 publications

Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

Advances in understanding of health‐promoting benefits of medicine and food homology using analysis of gut microbiota and metabolomics

Comparative Analysis of Nutritional Quality of Dendrobium Officinale Kimura Et Migo After Transplantation of Artificially Cultivated Plants From South to North China During Different Growing Years

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