Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from <i>Sinonovacula constricta</i>

Yuan, Qiuyan; Zhao, Longyan; Cha, Qian‐Qian; Sun, Yi; Ye, Hong; Zeng, Xiaoxiong

doi:10.1021/acs.jafc.5b03306

Cited by 124 publications

(49 citation statements)

References 71 publications

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“…() found that after treatment with the polysaccharide CMPB90‐1 from Cordyceps militaris at 250 to 500 μg/mL, a stimulated significant NO production in RAW 264.7 macrophages was observed and the level of production was similar to that production stimulated by LPS at 1 μg/mL. NO can be used as a quantitative index of macrophage activation and plays a pivotal role in host defense against invading microbes and tumors (Park et al., ; Yuan et al., ). Thus, this result indicated that KOS had a good effect on macrophage activation.…”

Section: Resultsmentioning

confidence: 99%

Prebiotic, Immunomodulating, and Antifatigue Effects of Konjac Oligosaccharide

Zeng

Zhang

et al. 2018

Journal of Food Science

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Irregular and long time work schedules not only makes people feel fatigue, but also brings great risks of diseases, due to gastrointestinal disorder and immune dysfunction. Therefore, it has positive significance to help challenged people stay energetic and healthy with food supplement. Konjac oligosaccharide has shown various physiological benefits and been recommended in the fortification of functional foods. However, there have been few reports on its application aimed to simultaneously relieve physical fatigue and keep body healthy. In this paper, the potential prebiotic, immunoregulatory, and antifatigue activities of konjac oligosaccharide were evaluated in vitro and in vivo. The results showed that konjac oligosaccharide could promote probiotics growth and short chain fatty acids production in mice cecum. At the concentration of 50 to 200 μg/mL, konjac oligosaccharide could activate murine macrophage RAW 264.7 to secret NO and cytokines of IL-10 and IL-6. Moreover, this oligosaccharide could alleviate physical fatigue by prolonging exhaustive time, improving the level of superoxide dismutases and glutathione peroxidase, increasing the content of blood glucose, and decreasing the content of blood urea nitrogen. The results suggested that konjac oligosaccharide had prebiotic, immunoregulatory, and antifatigue effects, providing its application potential in functional food aimed at people with irregular and long time work.

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Section: Resultsmentioning

confidence: 99%

Prebiotic, Immunomodulating, and Antifatigue Effects of Konjac Oligosaccharide

Zeng

Zhang

et al. 2018

Journal of Food Science

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“…Activation of macrophages can enhance their antitumor activity through secretion of cytokines or regulation of the immune system [ 29 ]; thereby, it is commonly assessed to examine the immunomodulatory function of polysaccharides [ 21 , 30 ]. In this study, macrophage immunological activity assay revealed that RS-3-1, BZ-3-1, FL-3-1, and GC-3-1 could enhance phagocytosis, promote NO production, and increase tumor necrosis factor (TNF- α ) level in RAW 264.7 cells.…”

Section: Resultsmentioning

confidence: 99%

Purification, Preliminary Characterization, and Immunological Activity of Polysaccharides from Crude Drugs of Sijunzi Formula

Wang

Peng

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Sijunzi Decoction (SJZD) is a conventional prescription for curing spleen deficiency in Traditional Chinese Medicine and polysaccharide is its main ingredient. In order to explore the effective ingredients contributing to the immunological activity of SJZD, we isolated and purified seven homogeneous polysaccharides from Radix Ginseng (RS-3-1 and RS-3-2), Rhizoma Atractylodis Macrocephalae (BZ-3-1, BZ-3-2, and BZ-3-3), Poria (FL-3-1), and Radix Glycyrrhizae (GC-3-1) decoctions, respectively. The molecular weight of seven homogeneous polysaccharides ranged from 5.42 × 104 to 5.65 × 104 Da. Monosaccharide composition determined by GC-MS analysis showed that these polysaccharides were primarily composed of Rha, Ara, Xyl, Man, Glc, and Gal with various ratios. Immunological activity assay revealed that polysaccharides from four crude drug components of SJZD displayed inhibitory effects on the complement system. RS-3-1, BZ-3-1, FL-3-1, and GC-3-1 could significantly enhance the phagocytosis and increase the NO production and tumor necrosis factor (TNF-α) level in RAW 264.7 cells (p < 0.05). These results demonstrated the immunological activities of these polysaccharides from the four crude drugs. This study supports the therapeutic effect of SJZD in clinical use and is essential for further identification the immunopolysaccharide from SJZD decoction.

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“…Overall, DPPY-0.5 possessed the most potent immunostimulating activity among the four polysaccharides. The immunomodulating activity of polysaccharides was found to be significantly influenced by their chemical compositions (Yuan et al, 2015). Sulfated polysaccharides have been demonstrated to possess superior immunomodulatory activity via in vitro and in vivo studies (Di et al, 2017;Kim et al, 2011).…”

Section: Effect On the Secretion Of No In Raw2647 Cellsmentioning

confidence: 99%

Purification, structural characterization, and biological activities of degraded polysaccharides from Porphyra yezoensis

et al. 2021

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The degraded polysaccharides from Porphyra yezoensis (DPPY) prepared using the H 2 O 2 -Vc method under optimized conditions were isolated and purified by DEAE Cellulose-52, and Sephadex G-100, providing four pure components, namely, DPPY-0, DPPY-0.1, DPPY-0.3, and DPPY-0.5. Their relative molecular weights were measured to be 10.8, 10.7, 18.7, and 35.5 kDa, respectively. GC-MS analysis revealed that all the four fractions were mainly composed of galactose, together with a small portion of glucose, mannose, xylose, and rhamnose. Structural analysis revealed that the purified polysaccharides mainly possess a backbone of ( 1(1 → 4)α-L-galactose-6-sulfate (G-L6S) units. They were found to promote the proliferation of RAW264.7 macrophages and enhance phagocytosis of the RAW264.7 cells. Antioxidant assays indicated that DPPY-0.5 possessed the most potent reducing power and free radical scavenging ability among the four purified polysaccharides. High sulfate content and proper molecular weight of these fractions are favorable to their immunomodulatory and antioxidant activities. Practical applicationsPorphyra yezoensis, common economic red algae widely distributed in East Asian countries, contains a high content of polysaccharides with a variety of biological activities. However, P. yezoensis polysaccharide (PPY) has not been well utilized due to the relatively low biological activities and lack of understanding of its structureactivity relationship. Thus, it is necessary to improve the bioactivities and elucidate the structure-activity relationship of this polysaccharide for its practical use. In the present work, four purified fractions (DPPY-0, DPPY-0.1, DPPY-0.3, and DPPY-0.5) were isolated from the degraded P. yezoensis polysaccharide, and were investigated for their antioxidant and immunoregulatory activities. The results of the present work will lay a foundation for the application of the degraded P. yezoensis polysaccharide in the food industry as a functional food ingredient.

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Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

Cited by 124 publications

References 71 publications

Prebiotic, Immunomodulating, and Antifatigue Effects of Konjac Oligosaccharide

Prebiotic, Immunomodulating, and Antifatigue Effects of Konjac Oligosaccharide

Purification, Preliminary Characterization, and Immunological Activity of Polysaccharides from Crude Drugs of Sijunzi Formula

Purification, structural characterization, and biological activities of degraded polysaccharides from Porphyra yezoensis

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