Structural characterization and in vitro antioxidant and hypoglycemic activities of degraded polysaccharides from sweet corncob

Ma, Yongqiang; Xiu, Weiye; Wang, Xin; Yu, Shiyou; Luo, Yan; Gu, Xiaoyu

doi:10.1016/j.jcs.2022.103579

Cited by 25 publications

(7 citation statements)

References 28 publications

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“…Figure 2 shows the absorption bands in FT‐IR spectra for RFCP and RACP. The absorption band at 3288 cm −1 is related to the presence of intermolecular hydrogen bonds in carbohydrates (O−H stretching vibration) [29] . The absorption band near 1920 cm −1 was attributed to C−H stretching vibration [22] .…”

Section: Resultsmentioning

confidence: 99%

“…A band between 260 and 280 nm in the UV-Vis spectra (Figure 1A), confirms the presence of proteins in RFCP and RACP. [29] Despite using the Sevag method for deproteinization of crude polysaccharides, the detected protein content remained at 4.00 � 0.11 for RFCP and 4.27 � 0.06 % for RACP. This suggests that proteins present in the extracts are bound to the polysaccharide.…”

Section: Uv-vis Spectroscopy Analysis and Monosaccharide Compositionmentioning

confidence: 97%

“…The absorption band at 3288 cm À 1 is related to the presence of intermolecular hydrogen bonds in carbohydrates (OÀ H stretching vibration). [29] The absorption band near 1920 cm À 1 was attributed to CÀ H stretching vibration. [22] The presence of uronic acids was related to the absorption band around 1720 cm À 1 (bending vibration of C=O).…”

Section: Congo Red Assaymentioning

confidence: 99%

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Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

Muñoz‐Castiblanco,

Santa Maria de la Parra,

Peña‐Cañón

et al. 2024

ChemistrySelect

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Water‐soluble crude polysaccharides (WSCPs) were isolated from the fruiting bodies of Ramaria flava (Schaeff.) Quél. (RFCP) and Ramaria cf. aurea (Schaeff.) Quél. (RACP). Species of Ramaria were collected in Boyacá‐Colombia. WSCPs were obtained by hot‐water extraction, ethanol precipitation, and dialysis and characterized using UV‐Vis spectroscopy, gel permeation chromatography, high‐performance liquid chromatography‐diode array detector, fourier transform infrared and nuclear magnetic resonance spectroscopy. Different assays evaluated the antioxidant capacity, and the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT)‐assay was used to determine cytotoxic effects. Chemical compositional analysis revealed that RFCP and RACP are heteropolysaccharides with molecular weights of 407 and 818 kDa, respectively compose mainly of glucose, mannose, galactose, fucose, and glucuronic acid with small amounts of protein and polyphenols. The backbone of RFCP and RACP is composed of β‐D‐Glcp‐(4→,→3)‐β‐D‐Glcp‐(1→,→2,6)‐α‐D‐Galp‐(1→,→3,6)‐α‐D‐Manp‐(1→. Antioxidant capacity demonstrated that both crude polysaccharides exhibit reducing power, radical scavenging ability, and a protective effect on lipid‐peroxidation. MTT assay showed that RFCP and RACP inhibited the growth of cancer cells HCT 116‐(colorectal) and MG‐63‐(bone) in a concentration‐dependent manner. Compared with RFCP, RACP showed better antioxidant capacity and cytotoxic effects due to differences in composition, molecular weight, and triple‐helical conformation. Overall, WSCPs exhibited a promising biological effect, highlighting the cultural, nutritional, and medicinal value of wild mushrooms.

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Section: Resultsmentioning

confidence: 99%

Section: Uv-vis Spectroscopy Analysis and Monosaccharide Compositionmentioning

confidence: 97%

Section: Congo Red Assaymentioning

confidence: 99%

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Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

Muñoz‐Castiblanco,

Santa Maria de la Parra,

Peña‐Cañón

et al. 2024

ChemistrySelect

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“…The molecular weight of poly-D-mannose was determined using the method of water-based size exclusion chromatography [ 26 , 27 ]. Briefly, a Waters 2695 HPLC system (Milford, MA, USA) equipped with a TSK gel GMPW XL column (300 mm × 7.8 mm, Tosoh Corp., Tokyo, Japan) and a refractive index detector were used.…”

Section: Methodsmentioning

confidence: 99%

A Poly-D-Mannose Synthesized by a One-Pot Method Exhibits Anti-Biofilm, Antioxidant, and Anti-Inflammatory Properties In Vitro

et al. 2023

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In this study, D-mannose was used to synthesize poly-D-mannose using a one-pot method. The molecular weight, degree of branching, monosaccharide composition, total sugar content, and infrared spectrum were determined. In addition, we evaluated the safety and bioactivity of poly-D-mannose including anti-pathogen biofilm, antioxidant, and anti-inflammatory activity. The results showed that poly-D-mannose was a mixture of four components with different molecular weights. The molecular weight of the first three components was larger than 410,000 Da, and that of the fourth was 3884 Da. The branching degree of poly-D-mannose was 0.53. The total sugar content was 97.70%, and the monosaccharide was composed only of mannose. The infrared spectra showed that poly-D-mannose possessed characteristic groups of polysaccharides. Poly-D-mannose showed no cytotoxicity or hemolytic activity at the concentration range from 0.125 mg/mL to 8 mg/mL. In addition, poly-D-mannose had the best inhibition effect on Salmonella typhimurium at the concentration of 2 mg/mL (68.0% ± 3.9%). The inhibition effect on Escherichia coli O157:H7 was not obvious, and the biofilm was reduced by 37.6% ± 2.9% at 2 mg/mL. For Staphylococcus aureus and Bacillus cereus, poly-D-mannose had no effect on biofilms at low concentration; however, 2 mg/mL of poly-D-mannose showed inhibition rates of 33.7% ± 6.4% and 47.5% ± 4%, respectively. Poly-D-mannose showed different scavenging ability on free radicals. It showed the best scavenging effect on DPPH, with the highest scavenging rate of 74.0% ± 2.8%, followed by hydroxyl radicals, with the scavenging rate of 36.5% ± 1.6%; the scavenging rates of superoxide anion radicals and ABTS radicals were the lowest, at only 10.1% ± 2.1% and 16.3% ± 0.9%, respectively. In lipopolysaccharide (LPS)-stimulated macrophages, poly-D-mannose decreased the secretion of nitric oxide (NO) and reactive oxygen species (ROS), and down-regulated the expression of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). Therefore, it can be concluded that poly-D-mannose prepared in this research is safe and has certain biological activity. Meanwhile, it provides a new idea for the development of novel prebiotics for food and feed industries or active ingredients used for pharmaceutical production in the future.

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“…We previously extracted sweet corn cob polysaccharides and investigated their functional properties [ 12 ]. Some researchers found that polysaccharide-iron (III) complexes would enhance bioactivity and could be used as a potential iron supplement.…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization, In Vitro Digestion Property, and Biological Activity of Sweet Corn Cob Polysaccharide Iron (III) Complexes

Xiu

Wang

et al. 2023

Molecules

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This study aimed to enhance the utilization value of sweet corn cob, an agricultural cereal byproduct. Sweet corn cob polysaccharide-ron (III) complexes were prepared at four different temperatures (40 °C, 50 °C, 60 °C, and 70 °C). It was demonstrated that the complexes prepared at different temperatures were successfully bound to iron (III), and there was no significant difference in chemical composition; and SCCP-Fe-C demonstrated the highest iron content. The structural characterization suggested that sweet corn cob polysaccharide (SCCP) formed stable β-FeOOH iron nuclei with −OH and −OOH. All the four complexes’ thermal stability was enhanced, especially in SCCP-Fe-C. In vitro iron (III) release experiments revealed that all four complexes were rapidly released and acted as iron (III) supplements. Moreover, in vitro antioxidant, α-glucosidase, and α-amylase inhibition studies revealed that the biological activities of all four complexes were enhanced compared with those of SCCP. SCCP-Fe-B and SCCP-Fe-C exhibited the highest in vitro antioxidant, α-glucosidase, and α-amylase inhibition abilities. This study will suggest using sweet corn cobs, a natural agricultural cereal byproduct, in functional foods. Furthermore, we proposed that the complexes prepared from agricultural byproducts can be used as a potential iron supplement.

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Structural characterization and in vitro antioxidant and hypoglycemic activities of degraded polysaccharides from sweet corncob

Cited by 25 publications

References 28 publications

Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

A Poly-D-Mannose Synthesized by a One-Pot Method Exhibits Anti-Biofilm, Antioxidant, and Anti-Inflammatory Properties In Vitro

Structural Characterization, In Vitro Digestion Property, and Biological Activity of Sweet Corn Cob Polysaccharide Iron (III) Complexes

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