Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

Hong, Tao; Zhao, Jiaying; Yin, Jingdong; Nie, Shaoping; Xie, Mingyong

doi:10.3389/fnut.2022.878249

Cited by 10 publications

(5 citation statements)

References 38 publications

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“…The 1 H NMR data are consistent with the 13 C NMR. In the low-field region of oxidized AG, typical signals of the C-6 carboxyl group of galacturonic acid units are observed at δ 173 and 174 ppm (esterified) and δ 170 ppm (non-esterified) [ 45 ]. In the anomeric region, overlapping signals at δ 100–108 ppm correspond to the C-1 galactose and arabinofuranose units in native AG, and after modification, galacturonic acid signals are added to the AG-TH spectrum ( Figure 11 b).…”

Section: Resultsmentioning

confidence: 99%

Inherited Structure Properties of Larch Arabinogalactan Affected via the TEMPO/NaBr/NaOCl Oxidative System

Ionin,

Malyar,

Borovkova

et al. 2024

Polymers

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Arabinogalactan (AG), extracted from larch wood, is a β-1,3-galactan backbone and β-1,6-galactan side chains with attached α-1-arabinofuranosyl and β-1-arabinopyranosyl residues. Although the structural characteristics of arabinogalactan II type have already been studied, its functionalization using 2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO) oxidation remains a promising avenue. In this study, the oxidation of AG, a neutral polysaccharide, was carried out using the TEMPO/NaBr/NaOCl system, resulting in polyuronides with improved functional properties. The oxidation of AG was controlled by analyzing portions of the reaction mixture using spectrophotometric and titration methods. To determine the effect of the TEMPO/NaBr/NaOCl system, air-dried samples of native and oxidized AG were studied by Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy, as well as by gel permeation chromatography. Compounds that model free (1,1-diphenyl-2-picrylhydrazyl (DPPH)) and hydroxyl radicals (iron(II) sulfate, hydrogen peroxide, and salicylic acid) were used to study the antioxidant properties. It was found that, in oxidized forms of AG, the content of carboxyl groups increases by 0.61 mmol compared to native AG. The transformation of oxidized AG into the H+ form using a strong acid cation exchanger leads to an increase in the number of active carboxyl groups to 0.76 mmol. Using FTIR spectroscopy, characteristic absorption bands (1742, 1639, and 1403 cm−1) were established, indicating the occurrence of oxidative processes with a subsequent reduction in the carboxyl group. The functionality of AG was also confirmed by gel permeation chromatography (GPC), which is reflected in an increase in molecular weights (up to 15,700 g/mol). A study of the antioxidant properties of the oxidized and protonated forms of AG show that the obtained antioxidant activity (AOA) values are generally characteristic of polyuronic acids. Therefore, the TEMPO oxidation of AG and other neutral polysaccharides can be considered a promising approach for obtaining compounds with the necessary controlled characteristics.

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Section: Resultsmentioning

confidence: 99%

Inherited Structure Properties of Larch Arabinogalactan Affected via the TEMPO/NaBr/NaOCl Oxidative System

Ionin,

Malyar,

Borovkova

et al. 2024

Polymers

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“…Signals corresponding to esterified methoxyl groups and the H2 of galacturonic residues appeared at 3.77 and 3.70 ppm, respectively [43]. The double peak observed between 4.45 and 4.35 ppm can be assigned to the H4 of galacturonic acid (4.40 ppm) and esterified galacturonic acid residues (4.46 ppm) [49]. In contrast, the SH-PEC spectrum exhibits a triple signal at 3.40 ppm, which corresponds to the protons of cysteamine (tagged as H1′) [50].…”

Section: Sh-pec Synthesismentioning

confidence: 98%

Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation

Beltran,

Luna,

Gastelum

et al. 2023

Pharmaceutics

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Gold nanorods (AuNRs) have attracted attention in the field of biomedicine, particularly for their potential as photothermal agents capable of killing tumor cells by photothermic ablation. In this study, the synthesis of novel AuNRs stabilized with thiolated pectin (AuNR@SH-PEC) is reported. To achieve this, thiolated pectin (SH-PEC) was obtained by chemically binding cysteamine motifs to the pectin backbone. The success of the reaction was ascertained using FTIR-ATR. Subsequently, the SH-PEC was used to coat and stabilize the surface of AuNRs (AuNR@SH-PEC). In this context, different concentrations of SH-PEC (0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 mg/mL) were added to 0.50 mL of AuNRs suspended in CTAB, aiming to determine the experimental conditions under which AuNR@SH-PEC maintains stability. The results show that SH-PEC effectively replaced the CTAB adsorbed on the surface of AuNRs, enhancing the stability of AuNRs without affecting their optical properties. Additionally, scanning electron and atomic force microscopy confirmed that SH-PEC is adsorbed into the surface of the AuNRs. Importantly, the dimension size (60 × 15 nm) and the aspect ratio (4:1) remained consistent with those of AuNRs stabilized with CTAB. Then, the photothermal properties of gold nanorods were evaluated by irradiating the aqueous suspension of AuNR@SH-PEC with a CW laser (808 nm, 1 W). These results showed that photothermal conversion efficiency is similar to the photothermal conversion observed for AuNR-CTAB. Lastly, the cell viability assays confirmed that the SH-PEC coating enhanced the biocompatibility of AuNR@SH-PEC. Most important, the viability cell assays subjected to laser irradiation in the presence of AuNR@SH-PEC showed a decrease in the cell viability relative to the non-irradiated cells. These results suggest that AuNRs stabilized with thiolated pectin can potentially be exploited in the implementation of photothermal therapy.

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“…5) Jujube polysaccharides' structural characteristics and structural kinds could be established by NMR and methylation studies, and the majority of them belonged to a pectin family that resembled homogalacturonan (HG). [44,45]…”

Section: Nmr Analysismentioning

confidence: 99%

Comparative Structural Characterization of Jujube Polysaccharides by Different Levels of Purification

Guo,

Shen,

Gao

et al. 2023

Starch Stärke

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The objective of this paper was to analyze and contrast the features of the structure that jujube polysaccharides extracted and purified using various techniques, four fractions, namely hot water extraction and 0.3 M NaCl‐PBS purification (PZMP3‐1 and PZMP3‐2), alkaline extraction and 0.3 M NaCl purification (SAZMP4), alkaline extraction and 0.3 M‐PBS NaCl purification (PZJRP), were obtained by column chromatography (Sephacryl S‐300) and Fast Flow (DEAE‐Sepharose). Chemical testing, gas chromatography, scanning electron microscopy, nuclear magnetic resonance spectroscopy, methylation analysis, high‐performance gel permeation chromatography, and atomic force microscopy were used to examine and evaluate their properties. Different purification methods had certain effects on the structural characteristics of a series of jujube polysaccharides. The results could provide fundamental knowledge to select desirable extraction and purification technologies for the preparation of pure jujube polysaccharides.This article is protected by copyright. All rights reserved

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Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

Cited by 10 publications

References 38 publications

Inherited Structure Properties of Larch Arabinogalactan Affected via the TEMPO/NaBr/NaOCl Oxidative System

Inherited Structure Properties of Larch Arabinogalactan Affected via the TEMPO/NaBr/NaOCl Oxidative System

Novel Gold Nanorods@Thiolated Pectin on the Killing of HeLa Cells by Photothermal Ablation

Comparative Structural Characterization of Jujube Polysaccharides by Different Levels of Purification

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