Structural Characterization of a Neutral Polysaccharide from Cucurbia moschata and Its Uptake Behaviors in Caco-2 Cells

Li, Fēi; Pak, SolJu; Zhao, Jing; Wei, Yunlu; Zhang, Yuyu; Li, Quanhong

doi:10.3390/foods10102357

Cited by 15 publications

(8 citation statements)

References 41 publications

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“…Polysaccharide absorption peaks were found at 800–1,200, 1,450–1,800, 2,900–3,000, and 3,200–3,600 cm –1 in the spectra. The hydroxyl group (O-H) had a characteristic significant broad stretch peak at 3,400.5 cm –1 , while the C-H stretching vibration had a band at 2,937.6 cm –1 ( 41 ). The broadband at 1633.7 cm –1 was created by bound water ( 18 ).…”

Section: Results and Analysismentioning

confidence: 99%

Hypoglycemic Effects of Lycium barbarum Polysaccharide in Type 2 Diabetes Mellitus Mice via Modulating Gut Microbiota

Zhai

Xie

et al. 2022

Front. Nutr.

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This study aims to explore the molecular mechanisms of Lycium barbarum polysaccharide (LBP) in alleviating type 2 diabetes through intestinal flora modulation. A high-fat diet (HFD) combined with streptozotocin (STZ) was applied to create a diabetic model. The results indicated that LBP effectively alleviated the symptoms of hyperglycemia, hyperlipidemia, and insulin resistance in diabetic mice. A high dosage of LBP exerted better hypoglycemic effects than low and medium dosages. In diabetic mice, LBP significantly boosted the activities of CAT, SOD, and GSH-Px and reduced inflammation. The analysis of 16S rDNA disclosed that LBP notably improved the composition of intestinal flora, increasing the relative abundance of Bacteroides, Ruminococcaceae_UCG-014, Intestinimonas, Mucispirillum, Ruminococcaceae_UCG-009 and decreasing the relative abundance of Allobaculum, Dubosiella, Romboutsia. LBP significantly improved the production of short-chain fatty acids (SCFAs) in diabetic mice, which corresponded to the increase in the beneficial genus. According to Spearman’s correlation analysis, Cetobacterium, Streptococcus, Ralstonia. Cetobacterium, Ruminiclostridium, and Bifidobacterium correlated positively with insulin, whereas Cetobacterium, Millionella, Clostridium_sensu_stricto_1, Streptococcus, and Ruminococcaceae_UCG_009 correlated negatively with HOMA-IR, HDL-C, ALT, AST, TC, and lipopolysaccharide (LPS). These findings suggested that the mentioned genus may be beneficial to diabetic mice’s hypoglycemia and hypolipidemia. The up-regulation of peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and insulin were remarkably reversed by LBP in diabetic mice. The real-time PCR (RT-PCR) analysis illustrated that LBP distinctly regulated the glucose metabolism of diabetic mice by activating the IRS/PI3K/Akt signal pathway. These results indicated that LBP effectively alleviated the hyperglycemia and hyperlipidemia of diabetic mice by modulating intestinal flora.

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Section: Results and Analysismentioning

confidence: 99%

Hypoglycemic Effects of Lycium barbarum Polysaccharide in Type 2 Diabetes Mellitus Mice via Modulating Gut Microbiota

Zhai

Xie

et al. 2022

Front. Nutr.

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“…EFP-3 has an absorption peak at 1,597.92 cm –1 , indicating the presence of N-H variable angle vibration in EFP-3 ( 49 ). The absorption peaks at 1,000 to 1,200 cm –1 were related to the C-O-C stretch of the glycoside bonds, which have different spectral shapes for polysaccharides composed of different monosaccharides, where multiple absorption bands appeared in EFP-2, EFP-3, and EFP-4, but only 1–2 absorption bands in EFP-0 and EFP-1 ( Figures 3A,B ); and it is noteworthy that EFP-0, EFP-2, EFP-3, and EFP-4 ( Figures 3A,C–E ) have absorption peaks at 1,000 to 1,050 cm –1 , indicating the presence of the pyranose ring in these four polysaccharide fractions ( 55 ), and 1,024 cm –1 is the peak of C-O-C stretching vibration ( 56 ). A peak around 900 cm –1 indicates the presence of β-glycoside bonds in the polysaccharide chain, but the peaks of EFP-0 and EFP-1 are weaker, and there is an absorption peak around 850 cm –1 indicating the presence of α-glycoside bonds in polysaccharides, but only EFP-0 has no absorption peak here ( 45 , 57 , 58 ) and ( 59 ).…”

Section: Resultsmentioning

confidence: 93%

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

et al. 2022

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The isolation, purification, and antioxidant activity of polysaccharides extracted from elderberry fruits were studied. Two neutral polysaccharides (EFP-0 and EFP-1) and three acidic polysaccharides (EFP-2, EFP-3, and EFP-4) were isolated from elderberry. EFP-0, EFP-1, EFP-2, EFP-3, and EFP-4 all contain arabinose, galactose, glucose, and mannose, with molecular weights of 1.7981 × 106, 7.0523 × 106, 7.7638 × 106, 4.3855 × 105, and 7.3173 × 105 Da, respectively. Structural characterization showed that the backbone of EFP-2 consisted of →4)-Manp (1→4)-β-D-Glcp (1→ and →4)-β-D-Glcp (1→5)-α-L-Araf (1→units, and T-α-L-Araf (1→ and T-β-D-Galp (1→ residues were detected by methylation analysis and NMR analysis. In addition, the MTT assay and zebrafish oxidative damage assay showed that EFP-2 had a protective effect on H2O2-damaged RAW264.7 cells in a dose-dependent manner, and zebrafish with the addition of EFP-2 would have low levels of ROS in vivo which showed significant antioxidant activity. Therefore, the results showed that the elderberry polysaccharides have antioxidant activity and can be used as potential antioxidants in functional foods.

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“…The spectra showed polysaccharide absorption peaks around 800–1,200, 1,300–1,800, and 3,200–3,600 cm −1 . At 3415.18 cm −1 , the hydroxyl group (O-H) displayed a recognizable broad stretch peak ( Li et al, 2021 ). The presence of uronic acid in RQP can be inferred from the strong band at 1657.42 cm −1 and the weak band at 1402.2 cm −1 caused by the absorption of a carboxylic group (COO−; Ye et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Anti-diabetic effect of red quinoa polysaccharide on type 2 diabetic mellitus mice induced by streptozotocin and high-fat diet

Zang,

Ge,

Cao

et al. 2024

Front. Microbiol.

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The purpose of this study was to explore the mechanism of red quinoa polysaccharide (RQP) in alleviating type 2 diabetes (T2D) through in vivo and in vitro experiments. Results of HPLC and FITR showed that RQP was a complex polysaccharide and contained more glucose, galactose and acarbose. In vitro experiments, RQP showed strong antioxidant capacity and inhibition on α-amylase and α-glucosidase. In vivo experiments, RQP was proved to induce a significant improvement of diabetes after 4 weeks of ingestion, including the abilities of lowering blood glucose, regulating lipid metabolism, anti-oxidation and promoting secretion of SCFAs. Furthermore, 16S rRNA study demonstrated that RQP transformed the intestinal microbiota composition in diabetic mice, decreased the abundance of norank_f_Muribaculaceae and Lachnospiraceae_NK4A136_group, and increased the relative abundance of Akkermansia, unclassified_f_Lachnospiraceae, norank_f_Eubacterium_coprostanoligenes_group, unclassified_f_Atopobiaceae and norank_f_Lachnospiraceae. The biosynthetic pathways, metabolic pathways and intestinal microbiome phenotypes in mice also changed accordingly. In conclusion, this study suggests that RQP can inhibit the development of diabetes by correcting the imbalance of intestinal flora.

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Structural Characterization of a Neutral Polysaccharide from Cucurbia moschata and Its Uptake Behaviors in Caco-2 Cells

Cited by 15 publications

References 41 publications

Hypoglycemic Effects of Lycium barbarum Polysaccharide in Type 2 Diabetes Mellitus Mice via Modulating Gut Microbiota

Hypoglycemic Effects of Lycium barbarum Polysaccharide in Type 2 Diabetes Mellitus Mice via Modulating Gut Microbiota

Extraction, isolation, structural characterization, and antioxidant activity of polysaccharides from elderberry fruit

Anti-diabetic effect of red quinoa polysaccharide on type 2 diabetic mellitus mice induced by streptozotocin and high-fat diet

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