Structural characterization of a novel derivative of myricetin from Mimosa pudica as an anti-proliferative agent for the treatment of cancer

Jose, Joby K.; Dhanya, A. T.; Haridas, Karickal R.; Kumar, T.M. Sumesh; Jayaraman, Sony; Variyar, E. Jayadevi; Sudhakaran, Sudheesh

doi:10.1016/j.biopha.2016.10.020

Cited by 37 publications

(16 citation statements)

References 17 publications

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“…With regards to our findings, EGCG, kaempferol, quercetin, and apigenin have previously been shown to regulate the Zn‐dependent HDACs . We report for the first time pan‐HDAC inhibition by myricetin (Table ), which has previously been shown to act as an antiproliferative agent for the treatment of cancer . Thus, we would speculate that anticarcinogenic actions of myricetin are mediated in part through HDAC inhibition.…”

Section: Discussionsupporting

confidence: 68%

Targeting the epigenome: Screening bioactive compounds that regulate histone deacetylase activity

Godoy

Lucas

Bender

et al. 2017

Molecular Nutrition Food Res

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Scope Nutrigenomics is a rapidly expanding field that elucidates the link between diet-genome interactions. Recent evidence demonstrates that regulation of the epigenome, and in particular inhibition of HDACs, impact pathogenetic mechanisms involved in chronic disease. Few studies, to date, have screened libraries of bioactive compounds that act as epigenetic modifiers. This study screened a library of 131 natural compounds to determine bioactive compounds that inhibit Zn-dependent HDAC activity. Methods and results Using class-specific HDAC substrates, we screened 131 natural compounds for HDAC activity in bovine cardiac tissue. From this screen, we identified 18 bioactive compound HDAC inhibitors. Using our class-specific HDAC substrates, we next screened these 18 bioactive compounds against recombinant HDAC proteins. Consistent with inhibition of HDAC activity, these compounds were capable of inhibiting activity of individual HDAC isoforms. Lastly, we report that treatment of H9c2 cardiac myoblasts with bioactive HDAC inhibitors was sufficient to increase lysine acetylation as assessed via immunoblot. Conclusion This study provided the first step in identifying multiple bioactive compound HDAC inhibitors. Taken together, this report sets the stage for future exploration of these bioactive compounds as epigenetic regulators to potentially ameliorate chronic disease.

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Section: Discussionsupporting

confidence: 68%

Targeting the epigenome: Screening bioactive compounds that regulate histone deacetylase activity

Godoy

Lucas

Bender

et al. 2017

Molecular Nutrition Food Res

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“…Myricetin has been demonstrated to be not only an effective antitumor medicine, but also a common ingredient of various foods and beverages (13). Jose et al (12) indicated that myricetin did not produce any toxicity in mice and may protect the cellular architecture of the liver through histopathology studies. Further studies on the effects of myricetin on EMT are required.…”

Section: Discussionmentioning

confidence: 99%

“…Myricetin is a flavonoid widely found in dicotyledonous plants, including onions, berries and grapes. It has been reported to have an extensive range of pharmacological activities, including antithrombotic (9), antioxidant (10), antitumor (7), anti-inflammatory, including reducing blood sugar (11) and protecting the liver and kidney (12). However, it has been reported that myricetin may have a toxic effect on cells (13).…”

Section: Introductionmentioning

confidence: 99%

Myricetin inhibits migration and invasion of hepatocellular carcinoma MHCC97H cell line by inhibiting the EMT process

Zhu

Ren

et al. 2019

Oncol Lett

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The recurrence and metastasis of hepatocellular carcinoma (HCC) are a major concern in current research. Epithelial-mesenchymal transition (EMT) is the leading cause underlying the high mobility and invasiveness of tumor cells. Myricetin is a natural flavonol with various pharmacological activities. The effects of myricetin on the migration and invasion of HCC MHCC97H cells were evaluated in the present study. Wound healing, Transwell migration and invasion assays were used to examine cell migration and invasion. Western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to examine the expression of epithelial (E)-cadherin, neural (N)-cadherin and vimentin. The present study aimed to investigate the effects of myricetin on the migration and invasion of HCC MHCC97H cells. It was indicated that myricetin decreased the viability of MHCC97H cells in a concentration and time-dependent manner, and inhibited MHCC97H cells migration and invasion. As the concentration of myricetin increased, filopodia and lamellipodia in cells weakened and cells were arranged more closely. RT-qPCR and western blotting revealed that myricetin upregulated E-cadherin expression and downregulated N-cadherin. Collectively, the results of the present study demonstrate that myricetin may inhibit the migration and invasion of HCC MHCC97H cells by inhibiting the EMT process.

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“…Its roots and leaves are used in hemorrhoids and fistula treatments and against convulsions (Hassan et al, 2010). In vitro and in vivo analysis of flavonoids and phenolic compounds of leaves, stems, and seeds of the M. pudica have found high antioxidant, antimicrobial, anticancer and antidiabetic activities, justifying its use in different diseases treatments (Doss et al, 2011;Kaur et al, 2011;Zhang et al, 2011;Joseph et al, 2013;Gunawardhana et al, 2015;Tunna et al, 2015;Jose et al, 2016;Muhammad et al, 2016;Patro et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

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This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies.

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Structural characterization of a novel derivative of myricetin from Mimosa pudica as an anti-proliferative agent for the treatment of cancer

Cited by 37 publications

References 17 publications

Targeting the epigenome: Screening bioactive compounds that regulate histone deacetylase activity

Targeting the epigenome: Screening bioactive compounds that regulate histone deacetylase activity

Myricetin inhibits migration and invasion of hepatocellular carcinoma MHCC97H cell line by inhibiting the EMT process

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

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