Structural characterization of a polysaccharide from the flower buds of Tussilago farfara, and its effect on proliferation and apoptosis of A549 human non-small lung cancer cell line

Qu, Honglan; Yang, Wei; Li, Jie

doi:10.1016/j.ijbiomac.2018.03.005

Cited by 36 publications

(9 citation statements)

References 34 publications

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“…Cell apoptosis was detected by Annexin V-FITC/propidium iodide (PI) staining assay using flow cytometry [36]. To analyze liposomes induced apoptosis of HepG2 cells, logarithmic phase cell concentration was adjusted to 1.5 × 105 cells/mL and inoculated in 6-well plates.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization of Liposomes Modified with Biosurfactant MEL-A Loading Betulinic Acid and Its Anticancer Effect in HepG2 Cell

Chen

2019

Molecules

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As a novel natural compound delivery system, liposomes are capable of incorporating lipophilic bioactive compounds with enhanced compound solubility, stability and bioavailability, and have been successfully translated into real-time clinical applications. To construct the soy phosphatidylcholine (SPC)–cholesterol (Chol) liposome system, the optimal formulation was investigated as 3:1 of SPC to Chol, 10% mannosylerythritol lipid-A (MEL-A) and 1% betulinic acid. Results show that liposomes with or without betulinic acid or MEL-A are able to inhibit the proliferation of HepG2 cells with a dose-effect relation remarkably. In addition, the modification of MEL-A in liposomes can significantly promote cell apoptosis and strengthen the destruction of mitochondrial membrane potential in HepG2 cells. Liposomes containing MEL-A and betulinic acid have exhibited excellent anticancer activity, which provide factual basis for the development of MEL-A in the anti-cancer applications. These results provide a design thought to develop delivery liposome systems carrying betulinic acid with enhanced functional and pharmaceutical attributes.

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Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization of Liposomes Modified with Biosurfactant MEL-A Loading Betulinic Acid and Its Anticancer Effect in HepG2 Cell

Chen

2019

Molecules

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“…An in vitro study showed that TFPB1 (0 to 1000 μg ml -1 ), a homogeneous polysaccharide with a molecular weight of 37.8 kDa, dose-dependently induced apoptosis and inhibited proliferation ( Table 5 ). Interestingly, TFPB1 (1 mg ml -1 ) could expansively arrest cell cycle in G2-M phase, hence it is worth noting that the underlying mechanism of different structural polysaccharides to further study on cell cycle ( Qu et al , 2018 ).…”

Section: Pharmacologymentioning

confidence: 99%

A review of the ethnobotanical value, phytochemistry, pharmacology, toxicity and quality control of Tussilago farfara L. (coltsfoot)

Chen

Dong

Quan

et al. 2021

Journal of Ethnopharmacology

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Ethnopharmacological relevance Tussilago farfara L. (commonly called coltsfoot), known as a vital folk medicine, have long been used to treat various respiratory disorders and consumed as a vegetable in many parts of the world since ancient times. Aim of the review This review aims to provide a critical evaluation of the current knowledge on the ethnobotanical value, phytochemistry, pharmacology, toxicity and quality control of coltsfoot, thus provide a basis for further investigations. Materials and methods A detailed literature search was obtained using various online search engines (e.g. Google Scholar, Web of Science, Science Direct, Baidu Scholar, PubMed and CNKI). Additional information was sourced from ethnobotanical literature focusing on Chinese and European flora. The plant synonyms were validated by the database ‘The Plant List’ ( www.theplantlist.org ). Results Coltsfoot has diverse uses in local and traditional medicine, but similarities have been noticed, specifically for relieving inflammatory conditions, respiratory and infectious diseases in humans. Regarding its pharmacological activities, many traditional uses of coltsfoot are supported by modern in vitro or in vivo pharmacological studies such as anti-inflammatory activities, neuro-protective activity, anti-diabetic, anti-oxidant activity. Quantitative analysis (e.g. GC-MS, UHPLC-MRM HR ) indicated the presence of a rich (>150) pool of chemicals, including sesquiterpenes, phenolic acids, flavonoids, chromones, pyrrolizidine alkaloids (PAs) and others from its leaves and buds. In addition, adverse events have resulted from a collection of the wrong plant which contains PAs that became the subject of public concern attributed to their highly toxic. Conclusions So far, remarkable progress has been witnessed in phytochemistry and pharmacology of coltsfoot. Thus, some traditional uses have been well supported and clarified by modern pharmacological studies. Discovery of therapeutic natural products and novel structures in plants for future clinical and experimental studies are still a growing interest. Furthermore, well-designed studies in vitro particularly in vivo are required to establish links between the traditional uses and bioactivities, as well as ensure safety before clinical use. In addition, the good botanical identification of coltsfoot and content of morphologically close species is a precondition for quality supervision and control. Moreover, strict quality control measures are required in the studies investigating any aspect of the pharmacology and chemistry of coltsfoot.

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“…The specific mechanism is that BRP promoted nitric oxide (NO) production and increased the secretion of TNF (Yao et al, 2017), and also increased the expression of death receptor DR5 and promoted pro-Caspase-3, pro-Caspase-8, pro-Caspase-9 and Bax cleavage, and reduced Bcl-2 (Wang et al, 2014). Qu et al found that a polysaccharide, TFPB1, from the flower buds of Tussilago farfara L. exerted a pro-apoptotic effect in human lung cancer A549 cells, while upregulating the expression of Fas, FasL, and Bax, and downregulating the expression of Bcl-2 is one of the reasons (Qu et al, 2018). Death receptor-mediated extrinsic apoptotic pathway was involved in the Panax ginseng C.A.Mey.…”

Section: Death Receptor Pathwaymentioning

confidence: 99%

Modulation of Apoptosis by Plant Polysaccharides for Exerting Anti-Cancer Effects: A Review

Gan

Wang

et al. 2020

Front. Pharmacol.

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Cancer has become a significant public health problem with high disease burden and mortality. At present, radiotherapy and chemotherapy are the main means of treating cancer, but they have shown serious safety problems. The severity of this problem has caused further attention and research on effective and safe cancer treatment methods. Polysaccharides are natural products with anti-cancer activity that are widely present in a lot of plants, and many studies have found that inducing apoptosis of cancer cells is one of their important mechanisms. Therefore, this article reviews the various ways in which plant polysaccharides promote apoptosis of cancer cells. The major apoptotic pathways involved include the mitochondrial pathway, the death receptor pathway, and their upstream signal transduction such as MAPK pathway, PI3K/AKT pathway, and NF-kB pathway. Moreover, the paper has also been focused on the absorption and toxicity of plant polysaccharides with reference to extant literature, making the research more scientific and comprehensive. It is hoped that this review could provide some directions for the future development of plant polysaccharides as anticancer drugs in pharmacological experiments and clinical researches.

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Structural characterization of a polysaccharide from the flower buds of Tussilago farfara, and its effect on proliferation and apoptosis of A549 human non-small lung cancer cell line

Cited by 36 publications

References 34 publications

Synthesis, Characterization of Liposomes Modified with Biosurfactant MEL-A Loading Betulinic Acid and Its Anticancer Effect in HepG2 Cell

Synthesis, Characterization of Liposomes Modified with Biosurfactant MEL-A Loading Betulinic Acid and Its Anticancer Effect in HepG2 Cell

A review of the ethnobotanical value, phytochemistry, pharmacology, toxicity and quality control of Tussilago farfara L. (coltsfoot)

Modulation of Apoptosis by Plant Polysaccharides for Exerting Anti-Cancer Effects: A Review

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