Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris

Lee, Jong Seok; Kwon, Jeong Seok; Yun, Jong Seok; Pahk, Jung Woon; Shin, Wan Seon; Lee, Shin Young; Hong, Eock Kee

doi:10.1016/j.carbpol.2010.01.017

Cited by 80 publications

(26 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since NO, IFN-c, and TNF-a play a critical in the antimicrobial and tumoricidal activities of macrophages, great attention has been paid to developing drugs that modulate NO and TNF-a production (Poderoso et al, 1999). Many polysaccharides can activate macrophages by inducing the production of cytokines (Belska et al, 2010;Lee et al, 2010;Togola et al, 2008). Our study showed that AMP promoted NO, TNF-a, and IFN-c secretion, indicating its stimulatory effect in the host defense against microbial infection on activated macrophages.…”

Section: Discussionmentioning

confidence: 60%

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Chen

Zheng

2014

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Atractylodes macrocephala Koidz is a traditional herb. Atractylodes macrocephalaon polysaccharides (AMP) have been found to enhance immunity and improve heart function. However, the mechanisms of the immunomodulatory effect have not been investigated. Objective: We examined whether AMP activated macrophages and explored the mechanisms of activation. Materials and methods: AMP was prepared and evaluated its immunomodulatory activity (25, 50, 100, and 200 lg/mL) by detecting the phagocytosis and the production of tumor necrosis factor-a (TNF-a), IFN-c, and nitric oxide (NO) in RAW264.7 macrophages. Furthermore, the role of nuclear factor-kB (NF-kB) pathway was examined in regulating TNF-a and NO production. Results: The phagocytosis of macrophages was enhanced by AMP in a dose-dependent manner and the maximal phagocytosis of macrophages occurred at concentrations of 100 and 200 lg/mL. NO, TNF-a, and IFN-c release was also found to be dose dependent by increasing concentrations of AMP and reached the peak at a concentration of 200 lg/mL. In addition, AMP induced inhibitor kappaB (IkB) degradation and the activation of NF-kB by p65 nuclear translocation, and then the activation of NF-kB in nucleus peaked at a concentration of 200 lg/ mL. Besides, NF-kB-specific inhibitor pyrrolidine dithiocarbamate (PDTC) decreased AMP-induced NO and TNF-a production. Discussion and conclusion: These data suggest that AMP may modulate macrophage activities by stimulating NF-kB or activating NF-kB-dependent mechanisms.

show abstract

Section: Discussionmentioning

confidence: 60%

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Chen

Zheng

2014

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…CPMN Fr II was a 1, 6-branched-glucogalactomannan with the β-linkage and random coil conformation (Lee et al, 2010a). CPMN Fr III was a β-1, 4-branched-β-1, 6galactoglucomannan (Lee et al, 2010b). The interpretation of polysaccharide structural differences from above results may relative to the fact that they were different C. militaris strains.…”

Section: Discussionmentioning

confidence: 71%

Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris

Wu¹,

Yan²,

Ma³

et al. 2011

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

The polysaccharide fraction, CM-hs-CPS2, was isolated from fruiting bodies of cultured Cordyceps militaris grown on solid rice medium by hot water extraction and ethanol precipitation, and purified by ion exchange column (DEAE-cellulose-52) and gel filtration column (Sephadex G-100) chromatography. Its structural characterizations were analyzed by gas chromatograph (GC) and fourier transform infrared spectroscopy (FT-IR). Results indicated that CM-hs-CPS2 was a kind of sulfating acidic polysaccharide containing acetamido group. The monosaccharide composition analysis showed that CM-hs-CPS2 was composed mainly of mannose, glucose and galactose in a molar ration of 1.35: 8.34: 1.00, and linked by α-glycosidic linkage. The studies on antioxidant activities of CM-hs-CPS2 were evaluated by various methods in vitro. Results showed that DPPH• scavenging activity, reducing power and ferrous ion chelating activity of CM-hs-CPS2 (8 mg/ml) were 89%, 1.188 and 85%, respectively.

show abstract

“…International Immunopharmacology j o u r n a l h o m e p a g e : w w w. e l s ev i e r. c o m / l o c a t e / i n t i m p 6B column chromatography, as previously reported [19]. The purified components of CM primarily consisted of polysaccharides, which was the (1)(2)(3)(4) …”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 97%

Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris

Lee

Hong

2011

International Immunopharmacology

Self Cite

119

View full text Add to dashboard Cite

Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris

Cited by 80 publications

References 36 publications

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris

Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris

Contact Info

Product

Resources

About