Structural characterization of Lanzhou lily (Lilium davidii var. unicolor) polysaccharides and determination of their associated antioxidant activity

Abstract

BACKGROUD: The Lanzhou lily (Lilium davidii var. unicolor) is the only Lilium species that is used for both culinary and medicinal purposes in China. Its bulbs contain various bioactive substances, such as polysaccharides, saponins and colchicine. Lanzhou lily polysaccharides are known to have anti-immunity, anti-tumor and anti-oxidation functions. RESULTS: The present study used a Box-Behnken design to optimize the ultrasound-assisted extraction of Lanzhou lily polysaccharides. Compared to other enzymes, trypsin significantly increased the polysaccharide yields, whereas the protein content of polysaccharides extracted with trypsin was the lowest. Monosaccharide mainly includes glucose (> 50%) and mannose (> 10%). 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity, chelating activity, total antioxidant capacity and hydroxyl radical scavenging activity of Lanzhou lily polysaccharides extracted with trypsin were stronger than those extracted without enzymes (control). Structural characteristics of Lanzhou lily polysaccharides extracted with trypsin and extracted without enzymes were characterized by scanning electron microscopy and nuclear magnetic resonance spectroscopy. When water extracted polysaccharide and trypsin extracted polysaccharide concentrations were 200 ∼g mL-1 , Raw264.7 proliferation rates were 101.69% and 159.41%, respectively. CONCLUSION: The Lanzhou lily polysaccharide was identified as ⊍-(1 → 6)-D-glucan. Consequently, the effects of both potential antioxidant and proliferative activity of trypsin are significant.

Effects of Lactobacillus plantarum Fermentation on the Chemical Structure and Antioxidant Activity of Polysaccharides from Bulbs of Lanzhou Lily

“…NMR spectroscopy is an important method used to analyze the structure of polysaccharides. The detailed structural information of polysaccharides could be analyzed by 1D and 2D NMR spectroscopy Figures and demonstrate the 13 C, 1 H, correlation spectroscopy (COSY), hetero-nuclear single quantum coherence (HSQC), and hetero-nuclear multiple-bond correlation spectroscopy (HMBC) spectra of the LP-W and LPF-W fractions.…”

“…21,34 NMR spectroscopy is an important method used to analyze the structure of polysaccharides. The detailed structural information of polysaccharides could be analyzed by 1D and 2D NMR spectroscopy 35 In the 1 H NMR spectra of LP-W (Figure 4a), the hydrogen spectrum signal is mainly concentrated between δ3.0 and 5.5 ppm. The δ3.2−4.0 ppm peaks are the sugar ring proton signals, and the group of δ4.3−5.5 ppm peaks is the sugar terminal proton signals, which occur at δ5.…”

Changes of starch and sucrose content and related gene expression during the growth and development of Lanzhou lily bulb

“…It is mainly grown in Gansu Province, China and is a very important agricultural plant with high economic value [ 2 , 3 ]. As a traditional medicinal plant and popular edible vegetable bulb, Lanzhou lily also has the function of clearing away heat and removing toxic, nourishing the lungs, anting cancer and enhancing human immunity [ 4 , 5 ].…”

Multi-Pharmaceutical Activities of Chinese Herbal Polysaccharides in the Treatment of Pulmonary Fibrosis: Concept and Future Prospects

“…LLP-1, LLP-2 and LLP-3 are the three new polysaccharide components in lily, their Mw are estimated to be 2.25 × 10 6 , 2.02 × 10 6 , and 2.08 × 10 6 Da, LLP-1 and LLP-2 are mainly composed of Glc and Man, with the molar ratio of 1:2 and 1:1, respectively, LLP-3 is mainly composed of Ara, Gal, Glc, and Man, with the molar ratio is 2:2:2:1 ( Chen et al, 2014 ; Hou et al, 2016 ). LLP has a variety of activities, such as hypoglycemic, anti-oxidant and anti-cancer effects ( Li et al, 2020b ). LLP can improve the PF alveolar compartment and reduce the infiltratsion of inflammatory cells.…”