Structural Characterization of Ulvan Polysaccharide from Cultivated and Collected <i>Ulva fasciata</i> (Chlorophyta)

Abstract

Ulvan is a sulfated heteropolysaccharide present in the cell wall of Ulva species with unique structural properties and technological potential. Here we characterized by FTIR and NMR analysis the structure of ulvan from Ulva fasciata collected in natural environment (SEA) and after in vitro biomass cultivation in nutrient enriched water (CULT). FTIR spectrum of CULT ulvan presented stronger signals of sulfate groups than SEA. 1 H and 13 C NMR showed that both ulvan are composed mainly of ulvanobiuronic acid 3-sulfate type A and type B. SEA ulvan presented signals characteristics of xylose, suggesting the presence of ulvanobiose in its structure, while CULT presented most signals of type A disaccharide. The cultivation of Ulva could be an alternative to suffice the emerging demand for ulvan meeting requirements of quality and quantity.

An Analysis of the Nutritional and Health Values of Caulerpa racemosa (Forsskål) and Ulva fasciata (Delile)—Two Chlorophyta Collected from the Philippines

“…According to Olasehinde et al [34], the presence of sulfate groups in algal polysaccharides are correlated to their biological activities or are synthesized in the algal Golgi bodies. These bands are due to these sugar cycles, according to Robic et al [36], and are a typical signal for ulvans [34][35][36]86]. The FTIR spectrum of U. fasciata crude ulvan extract confirmed the presence of sulfate groups and sugars.…”

“…The FTIR spectrum of U. fasciata crude ulvan extract confirmed the presence of sulfate groups and sugars. The presence, degree, and distribution of the sulfate groups are important in determining the biological activity of ulvan [86].…”

Antiviral activity of sulfated polysaccharides from marine algae and its application in combating COVID-19: Mini review

“…One of the most common disaccharide units, Ulvanobiuronic acid type A 3S consists of β- d -glucuronic acid (1,4)-linked to α-L rhamnose 3-sulfate, while in type B 3S α- l -iduronic acid is (1 → 4)-linked to α- l -rhamnose 3-sulfate. Ulvanobiose U 3S consists of β- d -xylose 2-sulfate (1 → 4)-linked to α- l -rhamnose 3-sulfate and type U 2 ' S,3S consist of β- d -xylose (1 → 4)-linked to α- l -rhamnose 3-sulfate ( Figueira et al, 2020 ). Some of the marine algae synthesized these sulfated polysaccharides.…”

Bioactive Compounds, Cosmeceutical And Nutraceutical Applications of Green Seaweed Species (Chlorophyta)

“…For instance, ulvans and xylans were found abundantly in Chlorophyta (Hentati et al, 2020;Kumar et al, 2008). In general, ulvan is differentiated from other seaweed polysaccharides because of the remarkable rare sugars rhamnose and iduronic acid, which are identical to the mammalian glycosaminoglycans (Figueira, da Silva, Enrich-Prast, Yoneshigue-Valentin, & de Oliveira, 2020). Meanwhile, xylans have a molecular structure of 1,4--D-xylans like higher plants (Aizatul, Abdul, Rahim, Yusof, & Atikah, 2021).…”

“…It is a sulfated polysaccharide that consists of a central framework of disaccharide modules, Lrhamnose 3-sulphate, which is linked to (i) ulvabiouronic acid unit A; (ii) ulvabiuronic acid unit B; (iii) ulvabiose unit A; or (iv) ulvabiose unit B (Shah et al, 2020). Moreover, ulvan is mainly composed of variable amounts of rhamnose, glucuronic acid, iduronic acid, xylose and high level of charged sulfated polyelectrolytes (Figueira et al, 2020;Aizatul et al, 2021). Ulva (family Ulvacea) from Chlorophyta sp.…”