2022
DOI: 10.1016/j.ijbiomac.2021.11.100
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Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva

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Cited by 25 publications
(12 citation statements)
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“…Besides, the connection manner of →4)- α -D-GalA p -(1→4)- α -D-GalA p -(1→ was speculated to exist through the HMBC correlation from C1 (δ 101.88) of H to H4 (δ 4.33) of F and C4 (δ 79.68) of F to H1 (δ 4.85) of H [ 47 , 48 ]. The correlation from C1 (δ 100.39) of F to H4 (δ 4.27) of E implied the presence of 4)- α -D-GalA p -(1→3,4)- α -D-Glc p -(1→ linkage [ 49 ]. The cross peaks between H1 (δ 5.08) of D and C4 (δ 78.18) of C, and H1 (δ 4.98) of G and C3 (δ 83.3) of E were observed in the HMBC spectrum, implying the presence of side chains of α -L-Ara f -(1→2,4)- α -L-Rha p -(1→ and α -L-Ara p -(1→3,4)-α-D-Glc p -(1→, respectively [ 32 , 50 ].…”
Section: Resultsmentioning
confidence: 99%
“…Besides, the connection manner of →4)- α -D-GalA p -(1→4)- α -D-GalA p -(1→ was speculated to exist through the HMBC correlation from C1 (δ 101.88) of H to H4 (δ 4.33) of F and C4 (δ 79.68) of F to H1 (δ 4.85) of H [ 47 , 48 ]. The correlation from C1 (δ 100.39) of F to H4 (δ 4.27) of E implied the presence of 4)- α -D-GalA p -(1→3,4)- α -D-Glc p -(1→ linkage [ 49 ]. The cross peaks between H1 (δ 5.08) of D and C4 (δ 78.18) of C, and H1 (δ 4.98) of G and C3 (δ 83.3) of E were observed in the HMBC spectrum, implying the presence of side chains of α -L-Ara f -(1→2,4)- α -L-Rha p -(1→ and α -L-Ara p -(1→3,4)-α-D-Glc p -(1→, respectively [ 32 , 50 ].…”
Section: Resultsmentioning
confidence: 99%
“…The shifts of H1–H5 and C1–C6 were obtained from the COSY spectrum and HSQC spectrum, respectively ( Figure 3 A,B). In addition, a C-6 shift was at around 175.41 ppm, and a C-4 shift at 78.40 ppm was a down-shift, which implied residue Ⅲ was 1,4-β-D-GlcpA [ 26 , 28 , 29 ].…”
Section: Resultsmentioning
confidence: 99%
“…Of interest, from the 13 C NMR, 1 H NMR, COSY NMR, and HSQC NMR of HU, we found some anomeric signals, and the chemical shifts of H/C were at 5.19/95.99, 4.91/98.13, 4.76/100.71, 5.11/98.13, 4.66/104.03, 4.85/103.10, and 5.15/100.24 ppm. Based on published literature and our results of the NMRs of U, we assigned the anomeric signals at 5.19/95.99, 4.91/98.13, 4.76/100.71, 5.11/98.13, 4.66/104.03, and 4.85/103.10 ppm to sugar residues Ⅰ (1,3-α-L-Rhap), Ⅱ (1,4-α-L-Rhap3S), Ⅲ (1,4-β-D-GlcpA), Ⅳ (1,4-β-D-Glcp), Ⅴ (1,4-β-D-Glcp3Me), and Ⅷ (1,4-α-L-IdopA) in HU [ 26 , 27 , 28 , 29 , 31 , 32 ]. Chemical shifts of other protons and carbon atoms of sugar residues were identified by analyzing the COSY and HSQC Ns of HU and listed in Table 2 .…”
Section: Resultsmentioning
confidence: 99%
“…However, the selection of pore size for ultrafiltration is not only based on the molecular size of ulvan but also depends on the elution amount (which decreasing with the pore size). In order to balance the influence of elution amount and pore size, the MWCO of about 10 kDa was generally selected (Glasson et al 2022(Glasson et al , 2017.…”
Section: Purification Methods Of Ulvanmentioning
confidence: 99%