Structural determination of the O-chain polysaccharide from the haloalkaliphilic Halomonas alkaliantarctica bacterium strain CRSS

Pieretti, Giuseppina; Nicolaus, Barbara; Poli, Aannarita; Corsaro, Maria Michela; Lanzetta, Rosa; Parrilli, Michelangelo

doi:10.1016/j.carres.2009.06.022

Cited by 14 publications

(11 citation statements)

References 29 publications

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“…Similar rhamnose-rich polysaccharides have been reported to be O-polysaccharide chains of lipopolysaccharides from P. syringae pv. phaseolicola (38) and Pseudomonas putida (43) and from strains of Xanthomonas campestris (39) and Halomonas alkaliantarctica (46). However, this is the first time that such branched rhamnans have been reported to be exopolysaccharides from Pseudomonas species.…”

Section: Discussionmentioning

confidence: 79%

Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Ghods

Sims

Moradali

et al. 2015

Appl Environ Microbiol

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c Pseudomonas syringae pv. actinidiae is the major cause of bacterial canker and is a severe threat to kiwifruit production worldwide. Many aspects of the disease caused by P. syringae pv. actinidiae, such as the pathogenicity-relevant formation of a biofilm composed of extracellular polymeric substances (EPSs), are still unknown. Here, a highly virulent strain of P. syringae pv. actinidiae, NZ V-13, was studied with respect to biofilm formation and architecture using a flow cell system combined with confocal laser scanning microscopy. The biofilm formed by P. syringae pv. actinidiae NZ V-13 was heterogeneous, consisting of a thin cellular base layer 5 m thick and microcolonies with irregular structures. The major component of the EPSs produced by P. syringae pv. actinidiae NZ V-13 bacteria was isolated and identified to be an exopolysaccharide. Extensive compositional and structural analysis showed that rhamnose, fucose, and glucose were the major constituents, present at a ratio of 5:1.5:2. Experimental evidence that P. syringae pv. actinidiae NZ V-13 produces two polysaccharides, a branched ␣-D-rhamnan with side chains of terminal ␣-D-Fucf and an ␣-D-1,4-linked glucan, was obtained. The susceptibility of the cells in biofilms to kasugamycin and chlorine dioxide was assessed. About 64 and 73% of P. syringae pv. actinidiae NZ V-13 cells in biofilms were killed when kasugamycin and chlorine dioxide were used at 5 and 10 ppm, respectively. Kasugamycin inhibited the attachment of P. syringae pv. actinidiae NZ V-13 to solid surfaces at concentrations of 80 and 100 ppm. Kasugamycin was bacteriostatic against P. syringae pv. actinidiae NZ V-13 growth in the planktonic mode, with the MIC being 40 to 60 ppm and a bactericidal effect being found at 100 ppm. Here we studied the formation, architecture, and composition of P. syringae pv. actinidiae biofilms as well as used the biofilm as a model to assess the efficacies of bactericidal compounds. P seudomonas syringae pv. actinidiae is the main cause of kiwifruit canker, which is epidemic worldwide (1-3). The P. syringae pv. actinidiae epidemic in New Zealand is threatening the kiwifruit industry, which is the second most important horticultural export crop by monetary value in New Zealand. In New Zealand, the highly virulent strain P. syringae pv. actinidiae V was reported in 2010 in the Bay of Plenty region and caused an estimated 50% loss of vines of the Hort16A kiwifruit variety known as Zespri Gold (4-6). This bacterial disease can affect all commercial species of kiwifruit (3,7,8). The main distinctive symptoms of disease are cankers on the vines and trunks, gummosis, dieback of the canes, wilting, and sometimes death of the vines (5).Similar to other pseudomonads, P. syringae pv. actinidiae can form cell communities known as biofilms. The formation of biofilms is a physiological response to environmental stresses mediated by the regulation of specific sets of genes, including genes encoding virulence factors. Biofilm formation protects cells against host defense me...

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Section: Discussionmentioning

confidence: 79%

Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Ghods

Sims

Moradali

et al. 2015

Appl Environ Microbiol

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“…Core OS and O‐chain moieties – but not lipid A – from H. alkaliantarctica strain CRSS, isolated from the saline lake Cape Russell in Antarctica, have been structurally resolved. The repeating unit of the O‐chain is composed of a trisaccharide of l ‐rhamnose, and the heptasaccharide core region possesses only one Kdo unit, phosphorylated at position O‐4 as depicted below.…”

Section: Lps In Gram‐negative Extremophiles: Structural and Biologmentioning

confidence: 99%

Gram‐Negative Extremophile Lipopolysaccharides: Promising Source of Inspiration for a New Generation of Endotoxin Antagonists

et al. 2017

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Extremophiles are microorganisms exhibiting the fascinating capability to thrive in habitats that are considered clearly inhospitable. Extreme-loving microorganisms have acquired ever-increasing importance in the biomedical and pharmaceutical fields, with many of their bioactive products having long been used as life-saving drugs. In this context, extremophile Gram-negative bacterial lipopolysaccharides (LPSs) and their analogues offer many promising opportunities for a variety of biomedical and therapeutic applications. The structure-dependent capability to elicit and to modulate host immune responses is surely the most intriguing feature of LPSs in the context of new drug discovery and design. This review offers an overview of the chemical peculiarities of LPSs isolated from extremophile bacteria, providing the most promising results relating to their biological activity. We discuss the pharmacologically important potential of extremophile LPSs as fundamental immunomodulatory compounds from the perspective of drug synthesis and development

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“…Fraction a, eluted in the void volume, was characterized as the O-chain. 13 Fractions b and c mainly contained the core oligosaccharide. The sugar analysis performed on these fractions showed the presence of D-glucose (Glc), D-glucuronic acid (GlcA), L-glycero-D-mannoheptose (L,D-Hep) and 2-keto-3-deoxy octulosonic acid (Kdo).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the spatial proximity of some protons was recognized by NOE contacts measured in a ROESY spectrum. Carbon chemical shifts were assigned utilizing 1 H, 13 C-DEPT-HSQC (Fig. 3) and 1 H, 13 C-HMBC.…”

Section: Resultsmentioning

confidence: 99%

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Structural characterization of the core region from the lipopolysaccharide of the haloalkaliphilic bacterium Halomonas alkaliantarctica strain CRSS

et al. 2010

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Halophilic and halotolerant Gram-negative bacteria are microorganisms which thrive in high salt environments. LPS are the major components of their outer leaflet, nevertheless very little is known about the role of this molecules in the adaptation mechanisms of extremophiles. Recently we determined the O-chain repeating unit structure of the LPS from Halomonas alkaliantarctica strain CRSS, an haloalkaliphilic Gram-negative bacterium isolated from salt sediments of a saline lake in Cape Russell in Antarctic continent. The polysaccharide is constituted of the trisaccharidic repeating unit: →3)-β-l-Rhap-(1→4)-α-l-Rhap-(1→3)-α-l-Rhap-(1→. In this paper we report the complete core LPS structure from this bacterium. The LPS was hydrolyzed both under mild acid and strong alkaline conditions. The MALDI spectra showed the presence of two glycoforms. The most abundant was recovered after HPAEC purification of the alkaline hydrolyzed product and was characterized by means of 2D-NMR spectroscopy. A comparison of the MALDI-PSD spectra of the two glycoforms suggested that the branched heptose was not stoichiometrically substituted.

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Structural determination of the O-chain polysaccharide from the haloalkaliphilic Halomonas alkaliantarctica bacterium strain CRSS

Cited by 14 publications

References 29 publications

Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide

Gram‐Negative Extremophile Lipopolysaccharides: Promising Source of Inspiration for a New Generation of Endotoxin Antagonists

Structural characterization of the core region from the lipopolysaccharide of the haloalkaliphilic bacterium Halomonas alkaliantarctica strain CRSS

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