Structural displacement model of chitooligosaccharide transport through chitoporin

Nanopore-based glycan sequencing: state of the art and future prospects

“…48,51 Early in the 1990s, some single-channel studies explored the transport mechanism of glycans via porins such as LamB 55 and chitooligosaccharide-uptake porins ChiP (chitoporin). 56 Although porins were proposed to be used as the earliest glycan sensors, 57 their low conductance, unavoidable gating signals, strong translocation specificity, and high blockage rate hindered their further application as glycan sensors. 57 By contrast, the general porins OmpF and OmpG with higher signal stability were developed as biosensors 58,59 and possibly applicable for glycan sensing.…”

“…Nevertheless, the natural transport mechanism of glycan-specific porins seems very enlightening for nanopore design. 56,60 For example, the asymmetric charge distribution within most glycan porins could enhance their sensing resolution. 60 The aromatic residues lining the pore work to assist the glycan translocation with their hydrophobic core.…”

Conformational flexibility driving charge-selective substrate translocation across a bacterial transporter

“…2,12,14 Some membrane pores, such as substrate-specific porins, contain a critical sensing zone that acts as a binding site promoting selective molecular translocation. 5,8,15–18 Although high-resolution structures are available, their molecular basis of selectivity remains underexplored. 1,3,15–18…”

“…5,8,15–18 Although high-resolution structures are available, their molecular basis of selectivity remains underexplored. 1,3,15–18…”