Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Ji, Xiaolong; Guo, Jianhang; Pan, Feibing; Kuang, Fengjun; Chen, Haiming; Guo, Xudan; Liu, Yanqi

doi:10.3389/fnut.2022.853115

Cited by 36 publications

(26 citation statements)

References 61 publications

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“…Two absorption bands (2,800–3,500 cm –1 and 500–1,700 cm –1 ) were clearly observed for the three polysaccharides. The appearance of a sharp and strong peak between 3,600 and 3,200 cm –1 is associated with -OH stretching due to the large number of intra-molecular and inter-molecular hydrogen bonds in samples ( 39 , 43 ). Peaks at 2,926 cm –1 are associated with asymmetric and symmetric stretching of CH 2 .…”

Section: Resultsmentioning

confidence: 99%

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

et al. 2022

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Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides. In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%. Three purified polysaccharide fractions were obtained after decolorization, deproteinization, and column chromatography. Then, their main composition and antioxidant activity were investigated. The dominant fraction was SHP-2 with a yield of 3.78%. It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others. The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked. This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

et al. 2022

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“…Therefore, the development of highly effective and non-toxic natural compounds is seen as a good alternative for the treatment of liver cancer. In recent years, many studies have shown that natural polysaccharides have significant inhibitory effects on tumors (3)(4)(5)(6). Natural polysaccharides have become the main research focus for anti-tumor drugs since they are less toxic and have no side effects in humans.…”

Section: Introductionmentioning

confidence: 99%

Anti-cancer Potential of Polysaccharide Extracted From Polygonatum sibiricum on HepG2 Cells via Cell Cycle Arrest and Apoptosis

Liu²,

Zhang³

et al. 2022

Front. Nutr.

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Polygonatum sibiricum is one of the most widely used traditional Chinese medicine in China. Polygonatum sibiricum polysaccharide (PSP) is the main functional component of Polygonatum sibiricum. In this study, a water-soluble polysaccharide (PSP-1) was first isolated from Polygonatum sibiricum with a molecular weight of 38.65 kDa. Structural analysis was performed via methylation and FT-IR spectroscopy analyses, which in combination with NMR spectroscopy, revealed that PSP-1 has a → 4-α-D-Glcp-1 → backbone with the substitution at O-6 with the β-D-Glcp-1 → residues. Furthermore, PSP-1 exhibited potent and concentration-dependent anticancer effects, inducing HepG2 cell apoptosis and arresting the cell cycle at the G1 phase. Moreover, PSP-1 also decreased the mitochondrial membrane potential, damaged the nucleus of HepG2 cells, and increased the activity of caspase-9 and−3 in the intrinsic apoptotic pathways to induce HepG2 cell apoptosis. To conclude, PSP-1 might be a good candidate for the treatment of liver cancer, and this work provides important information for understanding the relationship between structure and antitumor activity of PSP-1, which is relevant for the treatment of hepatocellular carcinoma in clinic.

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“…A Bruker 600 MHz spectrometer equipped with a 1 H/ 13 C double probe was used for NMR analysis at 25°C ( 18 ). An amount of 50 mg dried RATAPW was dissolved in 1 ml D 2 O and frozen three times to exchange H protons into deuterium completely.…”

Section: Methodsmentioning

confidence: 99%

Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii

Zhang

et al. 2022

Front. Nutr.

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Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease. Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored. In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii. In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii. The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%. The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches. Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway. Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes. RATAPW also enhances ConA-induced T cells and lipopolysaccharide (LPS)-induced B cell proliferation in a dose-dependent effect. Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.

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Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

Cited by 36 publications

References 61 publications

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

Anti-cancer Potential of Polysaccharide Extracted From Polygonatum sibiricum on HepG2 Cells via Cell Cycle Arrest and Apoptosis

Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii

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