Structural features, antioxidant and acetylcholinesterase inhibitory activities of polysaccharides from stem of Physalis alkekengi L.

Liu, Xuegui; Bian, Jıanpeng; Li, Danqi; Liu, Changfeng; Xu, Shuping; Zhang, Gongling; Zhang, Lixin; Gao, Pin‐Yi

doi:10.1016/j.indcrop.2018.12.047

Cited by 67 publications

(32 citation statements)

References 41 publications

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“…At present, there are few studies on the inhibitory activity of acetylcholinesterase in bee pollen. Acetylcholinesterase is involved in the development and maturation of cells and can promote the development of neurons and nerve regeneration [41].…”

Section: Results and Discussion Sections In Wrong Order—experimentmentioning

confidence: 99%

Bioactive Constituents of F. esculentum Bee Pollen and Quantitative Analysis of Samples Collected from Seven Areas by HPLC

Guo

Zhang

et al. 2019

Molecules

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Bee pollen contains all the essential amino acids needed by humans. China is the largest producer of bee pollen in the world. In the present study, we identified 11 fatty acids in F. esculentum bee pollen oil by GC-MS analysis, and 16 compounds were isolated from F. esculentum bee pollen by column chromatography and identified. A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was established for the quality control of F. esculentum bee pollen. A validated HPLC-DAD method was successfully applied to the simultaneous characterization and quantification of nine main constituents in seven samples collected from seven different areas in China. The results showed that all standard calibration curves exhibited good linearity (R2 > 0.999) in HPLC-DAD analysis with excellent precision, repeatability and stability. The total amount in the samples from the seven regions ranged from 23.50 to 46.05 mg/g. In addition, seven compounds were studied for their bioactivity using enzymic methods, whereby kaempferol (3) showed high α-glucosidase inhibitory activity (IC50: 80.35 μg/mL), ergosterol peroxide (8) showed high tyrosinase inhibitory activity (IC50: 202.37 μg/mL), and luteolin (1) had strong acetylcholinesterase inhibitory activity (IC50: 476.25 μg/mL). All results indicated that F. esculentum bee pollen could be a nutritious health food.

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Section: Results and Discussion Sections In Wrong Order—experimentmentioning

confidence: 99%

Bioactive Constituents of F. esculentum Bee Pollen and Quantitative Analysis of Samples Collected from Seven Areas by HPLC

Guo

Zhang

et al. 2019

Molecules

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“…The previous studies indicated that polysaccharides extracted from plants have some antioxidant activity and can be explored as a novel potential antioxidant [14][15][16]. Based on the separation and detection method, the analytical methods used for monosaccharide composition analysis of polysaccharides include gas chromatography [17], high-performance liquid chromatography (HPLC) [18], ion chromatography (IC) [19], and capillary electrophoresis (CE) [20]. Generally, polysaccharides are difficult to detect by conventional HPLC methods using UV or fluorescence detection because of their nonchromophoric attributes [21].…”

Section: Introductionmentioning

confidence: 99%

Extraction and chemical characterization of novel water-soluble polysaccharides from two palm species and their antioxidant and antitumor activities

Dawood

Elmongy

Negm

et al. 2020

Egyptian Journal of Basic and Applied Sciences

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Two crude heteropolysaccharides were isolated from fruits of Caryota mitis and Chamaerops humilis in percentages of 2.65 and 3.89, respectively. In the case of Caryota mitis (CM) polysaccharide, it consisted of galactose, glucose and mannose as the predominant monosaccharides. While mannose, galactose and arabinose were respectively the predominant monosaccharides in the structure of Chamaerops humilis (CH) crude polysaccharide. Sixteen common amino acids were recognized to be components of the protein-bound polysaccharides of CM and CH. The major amino acid in CM and CH polysaccharides was tyrosine (1.091ng/g) and arginine (0.684 ng/g), respectively. Antioxidant activities of tested polysaccharides were examined by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and ferric reducing activity power assay. In a concentration of 1 mg/ml, CM and CH polysaccharides recorded FRAP values of 0.56 and 0.44 µmol Fe ++ /g, respectively. The scavenging activity of CM sample was higher (IC 50 , 422.11 µg/ml) than that of CH polysaccharide (IC 50 , 630.0 µg/ml). Also, the antitumor activity of CM and CH polysaccharides was investigated using HepG2 and MCF-7 human cell lines. The polysaccharide of Caryota mitis had a higher antitumor activity than that of CHPs; it had IC 50 values of 13.6 and 46.4 µg/ml in HepG2 and MCF-7 assays, respectively.

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“…francheti on nitric oxide production. The antioxidant activity has been demonstrated both in fruits and in stems of Physalis alkekengi and was due to the radical scavenger ability and lipid peroxidation inhibition [ 46 , 47 ]. It is known that hyperglycemia is associated with a cascade of events which finally leads to endothelial disfunction, proinflammatory state, oxidative stress, atherosclerosis, alteration of insulin sensitivity and obesity.…”

Section: Discussionmentioning

confidence: 99%

Physalis alkekengi L. Extract Reduces the Oxidative Stress, Inflammation and Apoptosis in Endothelial Vascular Cells Exposed to Hyperglycemia

et al. 2020

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To find new natural remedies in diabetes, this study investigated the biological activity of two extracts obtained from the fruits (PhyF) and herba (PhyH) of Physalis alkekengi var. franchetii L. on human umbilical vein endothelial cells (HUVECs) exposed to normo- and hyperglycemic conditions. The biological effect was quantified by malondialdehyde, IL-31 and IL-33 levels in correlation with physico-chemical characterization and antioxidant activity. Additionally, from PhyP extract, the caspase-3, IL-6, IL-10, tumor necrosis factor (TNF)-α and nuclear transcription factor NFkB expressions were evaluated. HPLC analysis revealed a significant number of phenolic compounds, especially in PhyF extract, with a good antioxidant activity as highlighted by TEAC, CUPRAC or DPPH methods. On HUVECS cells, the extracts were not toxic even at high concentrations. Particularly PhyF extract, diminished lipid peroxidation and inhibited the IL-31 and IL-33 secretions induced by hyperglycemia. The inhibitory effect on proinflammatory cytokines was noticed after both doses of PhyF extract in parallel with the upregulation of anti-inflammatory cytokine IL-10. Moreover, PhyF, especially in a low dose, reduced caspase-3 active form. These experimental findings suggest that Physalis fruits extract exerted beneficial effects in hyperglycemia by inhibition of oxidative stress, inflammation and apoptosis being a good adjuvant option in diabetes.

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Structural features, antioxidant and acetylcholinesterase inhibitory activities of polysaccharides from stem of Physalis alkekengi L.

Cited by 67 publications

References 41 publications

Bioactive Constituents of F. esculentum Bee Pollen and Quantitative Analysis of Samples Collected from Seven Areas by HPLC

Bioactive Constituents of F. esculentum Bee Pollen and Quantitative Analysis of Samples Collected from Seven Areas by HPLC

Extraction and chemical characterization of novel water-soluble polysaccharides from two palm species and their antioxidant and antitumor activities

Physalis alkekengi L. Extract Reduces the Oxidative Stress, Inflammation and Apoptosis in Endothelial Vascular Cells Exposed to Hyperglycemia

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