Structural Features of a Full-Length Ubiquitin Ligase Responsible for the Formation of Patches at the Plasma Membrane

Knop, Jan; Lienemann, Tim; El-Kilani, Haifa; Krings, Catharina; Sindalovskaya, Maria; Bergler, Johannes; Betzel, Christian; Hoth, Stefan

doi:10.3390/ijms22179455

Cited by 2 publications

(1 citation statement)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Green fluorescence was observed, not only in the cytosol, but also in the nucleus (Figure 5). Interestingly, we frequently observed the fluorescent patches at the plasma membrane (PM) (Figure 5, see white arrows), These GFP patches may represent the sites for tethering the tonoplast and/or MVBs to the PM, as described in Arabidopsis [47][48][49]. In addition, mobile vesicles were also observed in the cytoplasm (Figure 5, see red arrows), suggesting that the function of SAUL1 homologues might be conserved in different plant species.…”

Section: Subcellular Localization Of Gmsaul1amentioning

confidence: 64%

Over-Expression of Soybean GmSAUL1 Enhances Disease Resistance in Nicotiana tabacum

et al. 2023

Agronomy

View full text Add to dashboard Cite

E3 ubiquitin ligases play essential roles in plant defense responses. However, their roles in other plant species have not been investigated extensively. Here, we used a gain-of-function approach to interrogate the function of GmSAUL1 (Senescence-Associated E3 Ubiquitin Ligase 1) homologs in soybeans. Ectopic over-expression of GmSAUL1a in Nicotiana tabacum resulted in autoimmune responses that could be suppressed by high temperature, which is a hallmark of NLR (nucleotide-binding leucine-rich repeat protein)-mediated resistance. Interestingly, an elevated activation of NtMPK6, but reduced activation of NtMPK4, responding to flg22 treatment, was observed in these GmSAUL1a-over-expressing tobacco lines, suggesting that over-expressing GmSAUL1a activates immune signaling through activating NtMPK6, but suppressing NtMPK4 kinase activity. Collectively, these results demonstrated that GmSAUL1 plays a positive and pivotal role in soybean immunity that is likely governed by NLR proteins.

show abstract

Section: Subcellular Localization Of Gmsaul1amentioning

confidence: 64%

Over-Expression of Soybean GmSAUL1 Enhances Disease Resistance in Nicotiana tabacum

et al. 2023

Agronomy

View full text Add to dashboard Cite

show abstract

Genome-wide identification and expression analysis of the U-box gene family related to biotic and abiotic stresses in Coffea canephora L.

Liu,

Chen

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Plant U-box genes play an important role in the regulation of plant hormone signal transduction, stress tolerance, and pathogen resistance; however, their functions in coffee ( Coffea canephora L.) remain largely unexplored. In this study, we identified 47 CcPUB genes in the C. canephora L. genome, clustering them into nine groups via phylogenetic tree. The CcPUB genes were unevenly distributed across the 11 chromosomes of C. canephora L., with the majority (11) on chromosome 2 and none on chromosome 8. The cis -acting elements analysis showed that CcPUB genes were involved in abiotic and biotic stresses, phytohormone responsive, and plant growth and development. RNA-seq data revealed diverse expression patterns of CcPUB genes across leaves, stems, and fruits tissues. qRT-PCR analyses under dehydration, low temperature, SA, and Colletotrichum stresses showed significant up-regulation of CcPUB2 , CcPUB24 , CcPUB34 , and CcPUB40 in leaves. Furthermore, subcellular localization showed CcPUB2 and CcPUB34 were located in the plasma membrane and nucleus, and CcPUB24 and CcPUB40 were located in the nucleus. This study provides valuable insights into the roles of PUB genes in stress responses and phytohormone signaling in C. canephora L., and provided basis for functional characterization of PUB genes in C. canephora L. Supplementary Information The online version contains supplementary material available at 10.1186/s12864-024-10745-w.

show abstract

Structural Features of a Full-Length Ubiquitin Ligase Responsible for the Formation of Patches at the Plasma Membrane

Cited by 2 publications

References 61 publications

Over-Expression of Soybean GmSAUL1 Enhances Disease Resistance in Nicotiana tabacum

Over-Expression of Soybean GmSAUL1 Enhances Disease Resistance in Nicotiana tabacum

Genome-wide identification and expression analysis of the U-box gene family related to biotic and abiotic stresses in Coffea canephora L.

Contact Info

Product

Resources

About