Structural features of pectic polysaccharides from stems of two species of Radix Codonopsis and their antioxidant activities

Zou, Yuanfeng; Zhang, Yanyun; Paulsen, Berit Smestad; Rise, Frode; Chen, Zhengli; Jia, Renyong; Li, Lixia; Song, Xu; Feng, Bin; Tang, Huaqiao; Huang, Chao; Yin, Zhongqiong

doi:10.1016/j.ijbiomac.2020.05.083

Cited by 56 publications

(78 citation statements)

References 49 publications

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“…Next, 200 μmol/mL of H 2 O 2 (Sigma-Aldrich, St. Louis, MO, USA) was added to each well ( n = 12), and the cells were cultured in a 37 °C incubator. After 24 h of incubation, 10 μL cell counting kit-8 (CCK8) (Wuhan Boster Biological Technology., Ltd., Wuhan, China) was added to each well, and after 1 h incubation, the absorbance was measured at 450 nm using a microwell reader (Bio-Rad, Software Version 8.0) [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

“…IPEC-J2 cells seeded onto 96-well plates were cultured in a 37 °C incubator for 24 h. Then, 200 μmol/mL H 2 O 2 was added to each well, and the cells were cultured for 24 h. Three concentrations of each polysaccharide fraction (5 μg/mL, 10 μg/mL, 20 μg/mL) were added to the 96-well plate. The cell viability was determined by the CCK8 method after incubation for 24 h at 37 °C [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

“…Cells were centrifuged at 12,000 rpm for 3 min after cell disruption to obtain the supernatant for the determination of antioxidant enzyme activity. The antioxidant enzyme activities were determined by a Biochemical Detection Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

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Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles

Zhu

et al. 2020

Molecules

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Truffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles. Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II. Additionally, two polysaccharide fractions were purified from T. pseudoexcavatum (TPD-NP and TPD-I). The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight. Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing. The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression. The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure. Further study on the structure-activity relationship is needed to be done.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles

Zhu

et al. 2020

Molecules

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“…Many traditional Chinese medicine books have mentioned that the origins of medicinal plants determine the traits and the efficacy of the medicinal materials 7 . In our previous study, it has been found that the pharmacological activities of different species of C. pilosula acidic polysaccharides are different 8 …”

Section: Introductionmentioning

confidence: 99%

“…A study focusing on secondary metabolites of the three species of Radix Codonopsis indicated that both types and content of low‐molecular‐weight compounds showed differences among the three species 25 . However, there are a few studies focusing on the structure and biological activities of polysaccharides from different species of Radix Codonopsis 8 . Previously, we studied the structure and bioactivity of a neutral polysaccharide fraction (CPPF) from C. pilosula Nannf.…”

Section: Introductionmentioning

confidence: 99%

Characterization of inulin‐type fructans from two species of Radix Codonopsis and their oxidative defense activation and prebiotic activities

et al. 2020

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BACKGROUND Codonopsis pilosula and C. tangshen are both plants widely used in traditional Chinese medicine. Polysaccharides, which are their primary active components, are thought to be important in their extensive use. In this study, two neutral polysaccharide fractions of C. pilosula (CPPN) and C. tangshen (CTPN) were obtained by fractionation on a DEAE–Sepharose column and characterized. RESULTS It was confirmed that the neutral polymers CPPN and CTPN were β‐(2,1)‐linked inulin‐type fructans with non‐reducing terminal glucose, and degree of polymerization (DP) of 19.6 and 25.2, respectively. The antioxidant and prebiotic activities in vitro were assayed based on IPEC‐J2 cell lines and five strains of Lactobacillus. Results indicated that the effects of CPPN and CTPN were increased antioxidant defense in intestinal epithelial cells through enhanced cell viability, improved expression of total antioxidant capacity, glutathione peroxidase, superoxide dismutase and catalase, and reduced levels of malondialdehyde and lactic dehydrogenase. The prebiotic activity of CPPN and CTPN was demonstrated by the promoting effect on Lactobacillus proliferation in vitro. The different biological activities obtained between the two fractions are probably due to the different DP and thus molecular weights of CPPN and CTPN. CONCLUSION The inulin fractions from C. pilosula and C. tangshen were natural sources of potential intestinal antioxidants as well as prebiotics, which will be valuable in further studies and new applications of inulin‐containing health products. © 2020 Society of Chemical Industry

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Effect of particle size on dissolution of different chemical components in Codonopsis pilosula

Huang,

Deng,

Zhao

et al. 2024

Biomedical Chromatography

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Codonopsis pilosula (Franch.) Nannf. is a traditional herb for treating immunosuppression. C. pilosula boiling powder (CP‐BP) contains particles of a small size made from C. pilosula decoction pieces (CP‐DP). It is still unclear how changes in particle size during the decoction process affect the dissolution of various chemical components in C. pilosula. Herein, an ultra‐high‐performance liquid chromatography–quadrupole‐Exactive Orbitrap mass spectrometry technique was established to characterize the components of CP‐BP and CP‐DP decoctions. The contents of the components were evaluated based on the relative peak area, extract yield, and alcohol solubility rate. A total of 71 compounds were finally identified, and their content in the CP‐BP decoction was generally higher than that in the CP‐DP decoction. Alkaloids had the highest average content, whereas terpenoids were the most affected by changes in particle size. In addition, immunosuppression was used as model to investigate whether these changes have practical significance. The results of network pharmacology suggested that the phosphoinositide 3‐kinase (PI3K)–Akt pathway may be a potential pathway of C. pilosula for treating immunosuppression. The results of molecular docking indicated that compounds with large content variations have good docking affinity with key targets (epidermal growth factor receptor [EGFR], prostaglandin‐endoperoxide synthase 2 [PTGS2], and peroxisome proliferator‐activated receptor gamma [PPARG]). These results provide an important reference for further development and use of C. pilosula.

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Structural features of pectic polysaccharides from stems of two species of Radix Codonopsis and their antioxidant activities

Cited by 56 publications

References 49 publications

Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles

Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles

Characterization of inulin‐type fructans from two species of Radix Codonopsis and their oxidative defense activation and prebiotic activities

Effect of particle size on dissolution of different chemical components in Codonopsis pilosula

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