Structural Insights into Polyhydroxyalkanoates Biosynthesis

Sagong, Hye-Young; Son, Hyeoncheol Francis; Choi, So Young; Lee, Sang Yup; Kim, Kyungjin

doi:10.1016/j.tibs.2018.08.005

Cited by 100 publications

(52 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The elucidation of structure/function relationships of the main actors of the PHA biosynthetic machineries, has opened the way to protein engineering approaches aimed at designing enzymes with tailored substrate specificity. This will ultimately lead to the development of engineered microorganisms producing tailor-made PHAs even from variable substrates, such as WFOs 26,29 . In this regard, the application of the process to waste streams derived from a defined source (big fast-food companies, restaurant chains), will assure a more stable PHA composition.…”

Section: Discussionmentioning

confidence: 99%

Conversion of no/low value waste frying oils into biodiesel and polyhydroxyalkanoates

Vastano

Corrado

Sannia

et al. 2019

Sci Rep

View full text Add to dashboard Cite

A sustainable bioprocess was developed for the valorization of a no/low value substrate, i.e. waste frying oils (WFOs) with high content of free fatty acids (FFAs), otherwise unsuitable for biodiesel production. The bioprocess was verified using both recombinant (Escherichia coli) and native (Pseudomonas resinovorans) polyhydroxyalkanoates (PHAs) producing cell factories. Microbial fermentation of WFOs provided a 2-fold advantage: i) the reduction of FFAs content resulting into an upgrading of the “exhausted waste oils” and ii) the production of a bio-based microbial polymer. Proper strain designing and process optimization allowed to achieve up to 1.5 g L−1 of medium chain length, mcl-PHAs, together with an efficient conversion (80% yield) of the treated WFO into biodiesel.

show abstract

Section: Discussionmentioning

confidence: 99%

Conversion of no/low value waste frying oils into biodiesel and polyhydroxyalkanoates

Vastano

Corrado

Sannia

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The PHAs are important thermoplastic polyesters produced by both Gram‐positive and Gram‐negative bacteria. They are thermoplastic polyesters originated from several R ‐hydroxyalkanoic acids . The classification of PHAs is given into three major types as short, medium, and long chain lengths.…”

Section: Polyhydroxyalkanoatesmentioning

confidence: 99%

“…They are thermoplastic polyesters originated from several R-hydroxyalkanoic acids. 63 The classification of PHAs is given into three major types as short, medium, and long chain lengths. The 3-hydroxyvalerate and 3-hydroxybutyrate are included in the class of short chain lengths.…”

Section: Polyhydroxyalkanoatesmentioning

confidence: 99%

Recent developments in the synthesis of poly(hydroxybutyrate) based biocomposites

Raza

Noor

Khalil

2019

Biotechnology Progress

View full text Add to dashboard Cite

Poly(hydroxybutyrate) (PHB) has become an attractive biomaterial in research and development for past few years. It is natural bio‐based aliphatic polyester produced by many types of bacteria. Due to its biodegradable, biocompatible, and eco‐friendly nature, PHB can be used in line with bioactive species. However, high production cost, thermal instability, and poor mechanical properties limit its desirable applications. So there is need to incorporate PHB with other materials or biopolymers for the development of some novel PHB based biocomposites for value addition. Many attempts have been employed to incorporate PHB with other biomaterials (or biopolymers) to develop sustainable biocomposites. In this review, some recent developments in the synthesis of PHB based biocomposites and their biomedical, packaging and tissue engineering applications have been focused. The development of biodegradable PHB based biocomposites with improved mechanical properties could be used to overcome its native limitations hence to open new possibilities for industrial applications.

show abstract

“…In butanoate metabolism, (3R)-3-hydroxybutanoyl-CoA can be converted into the polyhydroxyalkanoate (PHA) polybetahydroxybutrate (PHB). The PHAs are natural polyesters (Chen, 2010;Sagong et al, 2018;Verlinden et al, 2007). The synthesis of PHAs occurs in many bacteria under physiological stress, serving as an energy-and redox-storage material and allowing the organism to survive harsher environmental conditions (Chen, 2010;Lam et al, 2019;Sagong et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Crystal structure of acetoacetyl-CoA reductase fromRickettsia felis

et al. 2021

View full text Add to dashboard Cite

Rickettsia felis, a Gram-negative bacterium that causes spotted fever, is of increasing interest as an emerging human pathogen. R. felis and several other Rickettsia strains are classed as National Institute of Allergy and Infectious Diseases priority pathogens. In recent years, R. felis has been shown to be adaptable to a wide range of hosts, and many fevers of unknown origin are now being attributed to this infectious agent. Here, the structure of acetoacetyl-CoA reductase from R. felis is reported at a resolution of 2.0 Å. While R. felis acetoacetyl-CoA reductase shares less than 50% sequence identity with its closest homologs, it adopts a fold common to other short-chain dehydrogenase/reductase (SDR) family members, such as the fatty-acid synthesis II enzyme FabG from the prominent pathogens Staphylococcus aureus and Bacillus anthracis. Continued characterization of the Rickettsia proteome may prove to be an effective means of finding new avenues of treatment through comparative structural studies.

show abstract

Structural Insights into Polyhydroxyalkanoates Biosynthesis

Cited by 100 publications

References 106 publications

Conversion of no/low value waste frying oils into biodiesel and polyhydroxyalkanoates

Conversion of no/low value waste frying oils into biodiesel and polyhydroxyalkanoates

Recent developments in the synthesis of poly(hydroxybutyrate) based biocomposites

Crystal structure of acetoacetyl-CoA reductase fromRickettsia felis

Contact Info

Product

Resources

About