Structural modulation of gut microbiota during alleviation of antibiotic-associated diarrhea with herbal formula

Lv, Weijie; Liu, Cui; Ye, Chunxin; Sun, Jiaqi; Tan, Xin; Zhang, Chao; Qu, Qian; Shi, Dayou; Guo, Shining

doi:10.1016/j.ijbiomac.2017.02.060

Cited by 82 publications

(49 citation statements)

References 33 publications

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“…Dietary fiber benefits human health and can also modulate gut microbiota for treating diarrhea infections (63). Furthermore, the Chinese herbal formula SLBZS has been demonstrated to have an effect in shifting the gut microbiome structure during the treatment of rats with antibiotic-associated diarrhea (64). Taken together, the current study may provide a theoretical basis for gut microbiota and potential novel targets for the control of the disease.…”

Section: Discussionmentioning

confidence: 69%

Escherichia�coli O101‑induced diarrhea develops gut microbial dysbiosis in rats

et al. 2018

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Enterotoxigenic Escherichia coli (ETEC)-induced diarrhea is a devastating disease and one of the third leading causes of infectious disease-associated mortalities worldwide. Despite recent advances in the identification of the association between gut microbiota and diarrhea, a lack of understanding exists on the status of gut microbiota in rats treated with ETEC. In the present study, a rat model of Escherichia (E.) coli O 101 -induced diarrhea was established. The diarrhea incidence and index, as well as histological changes, were assessed. In addition, Illumina MiSeq sequencing of V3-V4 hypervariable regions of 16S ribosomal RNA was employed to investigate the changes in the gut microbiota profiles in the feces of the diarrhea rats. The results indicated that E. coli O 101 increased the diarrhea index and injury in the intestinal tissues, whereas it decreased the bacterial richness and shifted the distribution pattern of the bacterial communities in the phylum, order and genus levels in the fecal samples. Notably, the proportion of bacteria Prevotella, Enterococcus and Akkermansia was significantly decreased, while the pathogenic bacteria Escherichia/Shigella were significantly increased in diarrhea rats. Taken together, the gut microbiota is closely associated with E. coli O 101 -induced diarrhea in lower microbial diversity and dysbiosis of gut microbiota at different taxonomical levels.

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Section: Discussionmentioning

confidence: 69%

Escherichia�coli O101‑induced diarrhea develops gut microbial dysbiosis in rats

et al. 2018

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“…The rats were acclimatized for 7 days and then randomly divided into four groups (n = 6/group): the control (C) group, antibiotic-associated diarrhea (DM) group, natural recovery (NR) group, and WAP treatment (WAP) group. To establish the ADD model, rats on the DM, NR, and WAP groups received lincomycin hydrochloride (10 mL/kg) twice a day for 4 days by gavage [16], whereas rats in the C group received an equivalent amount of physiological saline. The rats of the DM group were anesthetized with isoflurane using a small animal anesthesia machine.…”

Section: Experimental Designmentioning

confidence: 99%

The Structure Features and Improving Effects of Polysaccharide from Astragalus membranaceus on Antibiotic-Associated Diarrhea

Ren

et al. 2019

Antibiotics

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Astragalus membranaceus (Astragalus) is often used as a medical and food resource in China. The present study was designed to investigate the features and effects of polysaccharide from Astragalus membranaceus (WAP) on rats with antibiotic-associated diarrhea (AAD). WAP was mainly composed of glucose, galactose, arabinose and glacturonic acid, with glucan, arabinogalactan and RG-I regions, and it showed loosely irregular sheet conformation. WAP decreased the inflammatory cell infiltration of colon in AAD rats, increased propionate and butyrate production, improved metabolic levels, adjusted the diversity and composition of gut microbiota, increased the relative abundance of Pseudomonas, and decreased the relative abundance of Allobaculum and Coprococcus. In conclusion, WAP contained different types of polysaccharide regions and sheet three-dimensional conformation, while it ameliorated AAD by recovering the colon structure, adjusting the gut microbiota, and improving the SCFAs levels. The results can provide some data basis for natural products to alleviate the side effects related to antibiotics.

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“…As diarrhea is a public health problem with potentially deadly effects, the WHO teamed up with other international organizations to launch a diarrheal disease control program with an emphasis on the use of traditional drug treatments to tackle the condition on a global scale (Xiong et al, 2018). Herbs and formulas in TCM can serve as drug discovery resources and valuable therapeutic strategies (Xu et al, 2015;Lv et al, 2017); however, according to the Chinese medicine theory, different types of diarrhea require different TCM formulas. Diarrhea can be divided into different forms such as dampness-heat, dampness-cold, spleen yang deficiency syndrome (Xiong et al, 2018) etc.…”

Section: Introductionmentioning

confidence: 99%

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Hua

Ma²,

Zhang

et al. 2020

Front. Pharmacol.

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Ethnopharmacological Relevance: Diarrhea is a major medical problem in clinical practice. According to the theory of traditional Chinese medicine (TCM), different types of diarrhea should be treated with different TCM formulations based on the targeted medical condition. Dampness-heat diarrhea (DHD) is a serious diarrheal disease and Pulsatilla decoction (PD), a TCM, has been found effective against DHD. Objective: The aim of this study was to clarify the mechanism of action of PD in DHD using an untargeted lipidomics strategy. Materials and Methods: Wistar rats were randomized to four groups, including the control group, model group, PD groups and self-healing group. The PD groups were given a daily intragastric gavage of PD at doses of 3.76 g/kg. The rat model of DHD established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment, drinking and intraperitoneal injection of Escherichia coli., which imitated the inducing conditions of DHD. Then the clinical symptoms and signs, blood routine, serum inflammatory cytokines levels and the histopathological changes of main organs were detected and observed to evaluate DHD model and therapeutic effect of PD. Lipid biomarkers of DHD were selected by comparing the control and model groups with the colon lipidomics technology and an ultra-high performance liquid chromatography (UHPLC) coupled with Q Exactive plus mass analyzer. Multivariate statistical analysis and pattern recognition were employed to examine different lipids within the colon of PD-treated rats. Results: The clinical symptoms and signs of the model rats were consistent with the diagnostic criteria of DHD. After treatment with PD, the clinical symptoms and signs of the rats with DHD were improved; the indexes of blood routine and inflammatory cytokines levels tended to be normal. The lipidomics profile of the model group were

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Structural modulation of gut microbiota during alleviation of antibiotic-associated diarrhea with herbal formula

Cited by 82 publications

References 33 publications

Escherichia�coli O101‑induced diarrhea develops gut microbial dysbiosis in rats

Escherichia�coli O101‑induced diarrhea develops gut microbial dysbiosis in rats

The Structure Features and Improving Effects of Polysaccharide from Astragalus membranaceus on Antibiotic-Associated Diarrhea

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

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