Structural Requirements for Interaction of Peroxisomal Targeting Signal 2 and Its Receptor PEX7

Kunze, Markus; Neuberger, Georg; Maurer‐Stroh, Sebastian; Ma, Jun; Eck, Thomas; Braverman, Nancy; Schmid, Johannes A.; Eisenhaber, Frank; Berger, Johannes

doi:10.1074/jbc.m111.301853

Cited by 50 publications

(56 citation statements)

References 65 publications

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“…In general, peroxisomal matrix proteins synthesized in the cytosol are bound by peroxisomal receptors – PEX523 in the case of PTS1, and PEX724 in the case of PTS2. These receptor–cargo complexes then move to the peroxisome membrane where they dock with protein sub-complexes that are in or on the membrane.…”

Section: Resultsmentioning

confidence: 99%

Pathway Compartmentalization in Peroxisome of Saccharomyces cerevisiae to Produce Versatile Medium Chain Fatty Alcohols

Sheng

Stevens

Feng

2016

Sci Rep

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Fatty alcohols are value-added chemicals and important components of a variety of industries, which have a >3 billion-dollar global market annually. Long chain fatty alcohols (>C12) are mainly used in surfactants, lubricants, detergents, pharmaceuticals and cosmetics while medium chain fatty alcohols (C6–C12) could be used as diesel-like biofuels. Microbial production of fatty alcohols from renewable feedstock stands as a promising strategy to enable sustainable supply of fatty alcohols. In this study, we report, for the first time, that medium chain fatty alcohols could be produced in yeast via targeted expression of a fatty acyl-CoA reductase (TaFAR) in the peroxisome of Saccharomyces cerevisiae. By tagging TaFAR enzyme with peroxisomal targeting signal peptides, the TaFAR could be compartmentalized into the matrix of the peroxisome to hijack the medium chain fatty acyl-CoA generated from the beta-oxidation pathway and convert them to versatile medium chain fatty alcohols (C10 & C12). The overexpression of genes encoding PEX7 and acetyl-CoA carboxylase further improved fatty alcohol production by 1.4-fold. After medium optimization in fed-batch fermentation using glucose as the sole carbon source, fatty alcohols were produced at 1.3 g/L, including 6.9% 1-decanol, 27.5% 1-dodecanol, 2.9% 1-tetradecanol and 62.7% 1-hexadecanol. This work revealed that peroxisome could be engineered as a compartmentalized organelle for producing fatty acid-derived chemicals in S. cerevisiae.

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Section: Resultsmentioning

confidence: 99%

Pathway Compartmentalization in Peroxisome of Saccharomyces cerevisiae to Produce Versatile Medium Chain Fatty Alcohols

Sheng

Stevens

Feng

2016

Sci Rep

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“…Secondary antibodies were goat ␣-mouse-HRP (Dako) and goat ␣-rabbit-HRP (Bio-Rad). All procedures were carried out as described previously (28). Detection was carried out using a Chemidoc XRSϩ detection system (Bio-Rad), and quantitative analysis was performed using Image Lab 4.0 analysis software.…”

Section: Methodsmentioning

confidence: 99%

Impaired Very Long-chain Acyl-CoA β-Oxidation in Human X-linked Adrenoleukodystrophy Fibroblasts Is a Direct Consequence of ABCD1 Transporter Dysfunction

Wiesinger

Kunze

Regelsberger

et al. 2013

Journal of Biological Chemistry

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126

121

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Background: ABCD1 is a peroxisomal ABC transporter whose dysfunction causes X-linked adrenoleukodystrophy (X-ALD). Results: ␤-Oxidation of C26:0 and C22:0 acyl-CoA esters is impaired in X-ALD. ABCD3 accounts for residual ␤-oxidation activity in X-ALD fibroblasts. Conclusion: ABCD1 mediates very long-chain acyl-CoA ester ␤-oxidation without need for additional re-esterification by an acyl-CoA synthetase. Significance: Our study provides proof of deficient acyl-CoA ester ␤-oxidation in X-ALD.

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“…Although PTS-1 and PTS-2 motif patterns are apparently well conserved in different phyla, correct prediction of proteins with peroxisomal targeting sequences is a challenging task. In silico predictions can be nonetheless done using computational methods; such analyses have been shown to be highly accurate in different systems (Hawkins et al, 2007;Kunze et al, 2011;Lingner et al, 2012;Opperdoes and Szikora, 2006).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Proteomics Analysis of Glycosomes fromLeishmania donovani

Jamdhade

Pawar

Chavan

et al. 2015

OMICS: A Journal of Integrative Biology

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Leishmania donovani is a kinetoplastid protozoan that causes a severe and fatal disease kala-azar, or visceral leishmaniasis. L. donovani infects human host after the phlebotomine sandfly takes a blood meal and resides within the phagolysosome of infected macrophages. Previous studies on host-parasite interactions have not focused on Leishmania organelles and the role that they play in the survival of this parasite within macrophages. Leishmania possess glycosomes that are unique and specialized subcellular microbody organelles. Glycosomes are known to harbor most peroxisomal enzymes and, in addition, they also possess nine glycolytic enzymes. In the present study, we have carried out proteomic profiling using high resolution mass spectrometry of a sucrose density gradient-enriched glycosomal fraction isolated from L. donovani promastigotes. This study resulted in the identification of 4022 unique peptides, leading to the identification of 1355 unique proteins from a preparation enriched in L. donovani glycosomes. Based on protein annotation, 566 (41.8%) were identified as hypothetical proteins with no known function. A majority of the identified proteins are involved in metabolic processes such as carbohydrate, lipid, and nucleic acid metabolism. Our present proteomic analysis is the most comprehensive study to date to map the proteome of L. donovani glycosomes.

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Structural Requirements for Interaction of Peroxisomal Targeting Signal 2 and Its Receptor PEX7

Cited by 50 publications

References 65 publications

Pathway Compartmentalization in Peroxisome of Saccharomyces cerevisiae to Produce Versatile Medium Chain Fatty Alcohols

Pathway Compartmentalization in Peroxisome of Saccharomyces cerevisiae to Produce Versatile Medium Chain Fatty Alcohols

Impaired Very Long-chain Acyl-CoA β-Oxidation in Human X-linked Adrenoleukodystrophy Fibroblasts Is a Direct Consequence of ABCD1 Transporter Dysfunction

Comprehensive Proteomics Analysis of Glycosomes fromLeishmania donovani

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