Structural study of phosphate groups in layered metal phosphates by high-resolution solid-state31P NMR spectroscopy

Eguchi, Taro; Nakamura, N.; Yamaguchi, Shunro; Danjyo, Mayumi; Tsuhako, Mitsutomo

doi:10.1039/a607807i

Cited by 81 publications

(53 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The coordination of phosphate/ phosphonate group to metal (IV), especially for Zr 4 + , has now been extensively illustrated in the literature 28,29 . Nakayama et al 30 also revealed the coordination of Ti 4 + with phosphate groups similar to Zr 4 + with phosphate groups using 31 P NMR. In this case, the MO6 are octahedrally coordinated by oxygen atoms, with the three oxygens of each phosphonate bound to three different zirconium ions 31 .…”

Section: Introductionmentioning

confidence: 99%

“…After this reaction, the phosphonate groups are coupled to the microspheres with a space arm of nine atoms, which facilitate the accessibility of the phosphopeptides. Finally, Ti 4 + is immobilized on the monodisperse microspheres via chelation with the phosphonate groups; each Ti 4 + ion bridges two phosphate oxygen atoms from two phosphonate groups present on the monodisperse microspheres 30,34 . The Ti 4 + -IMAC adsorbent prepared by this way has excellent performance in phosphopeptide enrichment, as demonstrated below.…”

Section: Experimental Design Preparation Of Immobilized Titanium (Iv)mentioning

confidence: 99%

See 1 more Smart Citation

Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography

et al. 2013

View full text Add to dashboard Cite

Mass spectrometry (Ms)-based proteomics has become the preferred tool for the analysis of protein phosphorylation. to be successful at such an endeavor, there is a requirement for an efficient enrichment of phosphopeptides. this is necessary because of the substoichiometric nature of phosphorylation at a given site and the complexity of the cell. recently, new alternative materials have emerged that allow excellent and robust enrichment of phosphopeptides. these monodisperse microsphere-based immobilized metal ion affinity chromatography (IMac) resins incorporate a flexible linker terminated with phosphonate groups that chelate either zirconium or titanium ions. the chelated zirconium or titanium ions bind specifically to phosphopeptides, with an affinity that is similar to that of other widely used metal oxide affinity chromatography materials (typically tio 2 ). Here we present a detailed protocol for the preparation of monodisperse microsphere-based ti 4 + -IMac adsorbents and the subsequent enrichment process. Furthermore, we discuss general pitfalls and crucial steps in the preparation of phosphoproteomics samples before enrichment and, just as importantly, in the subsequent mass spectrometric analysis. Key points such as lysis, preparation of the chromatographic system for analysis and the most appropriate methods for sequencing phosphopeptides are discussed. Bioinformatics analysis specifically relating to site localization is also addressed. Finally, we demonstrate how the protocols provided are appropriate for both single-protein analysis and the screening of entire phosphoproteomes. It takes ~2 weeks to complete the protocol: 1 week to prepare the ti 4 + -IMac material, 2 d for sample preparation, 3 d for Ms analysis of the enriched sample and 2 d for data analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Experimental Design Preparation Of Immobilized Titanium (Iv)mentioning

confidence: 99%

Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography

et al. 2013

View full text Add to dashboard Cite

show abstract

“…1. In studies of the isotropic 31 P chemical shifts of metal phosphates, Nakayama et al [27] found that the shifts of (H 2 PO 4 ) − , (HPO 4 ) 2− , and (PO 4 ) 3− groups occur at around δ −10, −20 and −30 ppm respectively. Subsequent studies have confirmed these findings [28,29].…”

Section: Characterisationmentioning

confidence: 98%

Proton conduction mechanism and the stability of sol–gel titanium phosphates

et al. 2007

View full text Add to dashboard Cite

“…3). On the basis of the previous studies in zirconium or titanium phosphates [13][14][15], we assigned the NMR peaks at −14.7 ppm to the HPO 4 group and −24.6 ppm to the PO 4 group. Considering these results it was concluded that AlZrPO-8 contains (HPO 4 ) and (PO 4 ) groups in the ratio of 1:3.…”

Section: Characterizationmentioning

confidence: 99%

“…3 and 4, respectively. Investigations [14]. The 31 P MAS NMR spectrum of AlZrPO-8 gives two peaks at −14.7 and −24.6 ppm with a relative intensity ratio of 1:3 (Fig.…”

Section: Characterizationmentioning

confidence: 99%

Hydrothermal synthesis and characterization of microporous crystals of trivalent metal-containing zirconium phosphates

Sun

Liu

et al. 2004

Catalysis Today

View full text Add to dashboard Cite

Structural study of phosphate groups in layered metal phosphates by high-resolution solid-state31P NMR spectroscopy

Cited by 81 publications

References 11 publications

Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography

Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography

Proton conduction mechanism and the stability of sol–gel titanium phosphates

Hydrothermal synthesis and characterization of microporous crystals of trivalent metal-containing zirconium phosphates

Contact Info

Product

Resources

About