Structural variants and modifications of hammerhead ribozymes targeting influenza A virus conserved structural motifs

Czapik, Tomasz; Piasecka, Julita; Kierzek, Ryszard; Kierzek, Elżbieta

doi:10.1016/j.omtn.2022.05.035

Cited by 3 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, modifications performed on the ribozymes require further investigation. Czapik et al showed that modifications such as adding a hairpin motif to the hammerhead ribozyme decreased their catalytic activity compared with the unmodified ribozymes [79].…”

Section: Stability and Delivery Of Ribozymesmentioning

confidence: 99%

Ribozymes as Therapeutic Agents against Infectious Diseases

Wong¹,

Shahid²,

Tan³

2023

RNA Therapeutics - History, Design, Manufacturing, and Applications

View full text Add to dashboard Cite

Ribozymes, also known as RNA enzymes, are catalytic RNA molecules capable of cleaving specific RNA sequences, leading to decreased expression of targeted genes. Recent studies suggest their role in cancer therapeutics, genetic diseases and retroviral infections. This book chapter will focus on ribozymes acting as therapeutic agents against infectious diseases caused by viral and bacterial pathogens. Firstly, we will introduce a brief history of ribozymes and a general overview of ribozymes and their characteristics. Next, different types of ribozymes will be explored regarding their targets and mechanisms of action. After that, ribozymes specific to viral and bacterial infections will be explored. We will briefly discuss the current status of ribozymes as therapeutic agents. Finally, the roadblock and challenges ribozymes face before being developed into therapeutic agents—such as their delivery and efficacy issues—will be discussed.

show abstract

Section: Stability and Delivery Of Ribozymesmentioning

confidence: 99%

Ribozymes as Therapeutic Agents against Infectious Diseases

Wong¹,

Shahid²,

Tan³

2023

RNA Therapeutics - History, Design, Manufacturing, and Applications

View full text Add to dashboard Cite

show abstract

“…Interesting observations were made by Czapik et al, who proposed new ribozyme hammerhead variants targeting conserved structural motifs in RNA segment 5 (+) of influenza virus strain A/California/04/2009 (H1N1) [ 105 ]. The variants were enriched with structural and chemical modifications; an additional hairpin motif was introduced as well as an attempt was made to select ribozyme target pairs with sequence features that enable the potential formation of the trans-Hoogsteen interactions.…”

Section: Influenza a Virusmentioning

confidence: 99%

“…Moreover, qRT-PCR analysis showed a significant decrease in viral RNA following the use of plasmids with ribozyme constructs enriched with the hairpin structure. The lowest viral titer was achieved with the use of the chimeric sh613RZ6C construct [ 105 ]. This use of ribozymes is promising, as it may allow the future development of constructs targeting important structural domains to achieve significant antiviral effects.…”

Section: Influenza a Virusmentioning

confidence: 99%

Structural and Functional RNA Motifs of SARS-CoV-2 and Influenza A Virus as a Target of Viral Inhibitors

Szczesniak

Baliga-Gil

Jarmolowicz

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the COVID-19 pandemic, whereas the influenza A virus (IAV) causes seasonal epidemics and occasional pandemics. Both viruses lead to widespread infection and death. SARS-CoV-2 and the influenza virus are RNA viruses. The SARS-CoV-2 genome is an approximately 30 kb, positive sense, 5′ capped single-stranded RNA molecule. The influenza A virus genome possesses eight single-stranded negative-sense segments. The RNA secondary structure in the untranslated and coding regions is crucial in the viral replication cycle. The secondary structure within the RNA of SARS-CoV-2 and the influenza virus has been intensively studied. Because the whole of the SARS-CoV-2 and influenza virus replication cycles are dependent on RNA with no DNA intermediate, the RNA is a natural and promising target for the development of inhibitors. There are a lot of RNA-targeting strategies for regulating pathogenic RNA, such as small interfering RNA for RNA interference, antisense oligonucleotides, catalytic nucleic acids, and small molecules. In this review, we summarized the knowledge about the inhibition of SARS-CoV-2 and influenza A virus propagation by targeting their RNA secondary structure.

show abstract

Evolution of the RNA world: From signals to codes

Spirov

2023

Biosystems

View full text Add to dashboard Cite

Structural variants and modifications of hammerhead ribozymes targeting influenza A virus conserved structural motifs

Cited by 3 publications

References 55 publications

Ribozymes as Therapeutic Agents against Infectious Diseases

Ribozymes as Therapeutic Agents against Infectious Diseases

Structural and Functional RNA Motifs of SARS-CoV-2 and Influenza A Virus as a Target of Viral Inhibitors

Evolution of the RNA world: From signals to codes

Contact Info

Product

Resources

About