Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3

Fry, Michelle Y.; Najdrová, Vladimíra; Maggiolo, Ailiena O.; Saladi, Shyam; Doležal, Pavel; Clemons, William M.

doi:10.1038/s41594-022-00798-4

Cited by 11 publications

(12 citation statements)

References 92 publications

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“…The lowest-resolution (8.46 Å) map in the test set is of the closed conformation of the ER targeting factor Get3: EMDB entry 25375 (Fry et al ., 2022). The authors did not deposit coordinates in the PDB for this reconstruction, presumably because it had the lowest resolution of a series of maps.…”

Section: Resultsmentioning

confidence: 99%

Likelihood-based docking of models into cryo-EM maps

Millán

McCoy²,

Terwilliger

et al. 2022

Preprint

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Optimized docking of models into cryo-EM maps requires exploiting expected signal in the data to minimize the calculation time while maintaining sufficient signal. The likelihood-based rotation function used in crystallography can be employed to establish plausible orientations in a docking search. A phased likelihood translation function yields scores for the placement and rigid-body refinement of oriented models. Optimised strategies for choices of the resolution of data and the size of search volumes are based on expected log-likelihood-gain scores, computed in advance of the search calculation. Tests demonstrate that the new procedure is fast, robust and effective at placing models into even challenging cryo-EM maps.

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Section: Resultsmentioning

confidence: 99%

Likelihood-based docking of models into cryo-EM maps

Millán

McCoy²,

Terwilliger

et al. 2022

Preprint

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show abstract

“…Get3, closed conformation. The lowest resolution (8.46 A ˚) map in the test set is of the closed conformation of the ER targeting factor Get3: EMDB entry EMD-25375 (Fry et al, 2022). The authors did not deposit coordinates in the PDB for this reconstruction, presumably because it had the lowest resolution of a series of maps.…”

Section: Cystic Fibrosis Transmembrane Regulator Df508mentioning

confidence: 99%

“…We chose the crystal structure the same authors determined for the open conformation, from PDB entry 7spz (Fry et al, 2022), as the model. The reconstruction is pseudosymmetric, so there are two independent copies to find.…”

Section: Cystic Fibrosis Transmembrane Regulator Df508mentioning

confidence: 99%

Likelihood-based docking of models into cryo-EM maps

Millán

McCoy

Terwilliger

et al. 2023

Acta Crystallogr D Struct Biol

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Optimized docking of models into cryo-EM maps requires exploiting an understanding of the signal expected in the data to minimize the calculation time while maintaining sufficient signal. The likelihood-based rotation function used in crystallography can be employed to establish plausible orientations in a docking search. A phased likelihood translation function yields scores for the placement and rigid-body refinement of oriented models. Optimized strategies for choices of the resolution of data from the cryo-EM maps to use in the calculations and the size of search volumes are based on expected log-likelihood-gain scores computed in advance of the search calculation. Tests demonstrate that the new procedure is fast, robust and effective at placing models into even challenging cryo-EM maps.

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“…Finally, TA binding on Get3 activates the ATPase activity of this targeting factor ( Figure 4 A, step 6) [ 92 ]. ATP hydrolysis induces the detachment of TA-loaded Get3 from Get4/5 and favors its interaction with the Get1/2 receptors instead, driving the movement of the targeting complex from the cytosol to the ER membrane [ 96 , 97 ]. Thus, the GET pathway is accomplished by a sequential series of energetically downhill molecular events.…”

Section: An Hsp70-cochaperone Cascade Guides Tas To the Ermentioning

confidence: 99%

Role of Hsp70 in Post-Translational Protein Targeting: Tail-Anchored Membrane Proteins and Beyond

Shan

2023

IJMS

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The Hsp70 family of molecular chaperones acts as a central ‘hub’ in the cell that interacts with numerous newly synthesized proteins to assist in their biogenesis. Apart from its central and well-established role in facilitating protein folding, Hsp70s also act as key decision points in the cellular chaperone network that direct client proteins to distinct biogenesis and quality control pathways. In this paper, we review accumulating data that illustrate a new branch in the Hsp70 network: the post-translational targeting of nascent membrane and organellar proteins to diverse cellular organelles. Work in multiple pathways suggests that Hsp70, via its ability to interact with components of protein targeting and translocation machineries, can initiate elaborate substrate relays in a sophisticated cascade of chaperones, cochaperones, and receptor proteins, and thus provide a mechanism to safeguard and deliver nascent membrane proteins to the correct cellular membrane. We discuss the mechanistic principles gleaned from better-studied Hsp70-dependent targeting pathways and outline the observations and outstanding questions in less well-studied systems.

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Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3

Cited by 11 publications

References 92 publications

Likelihood-based docking of models into cryo-EM maps

Likelihood-based docking of models into cryo-EM maps

Likelihood-based docking of models into cryo-EM maps

Role of Hsp70 in Post-Translational Protein Targeting: Tail-Anchored Membrane Proteins and Beyond

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