Structure and antimicrobial activity relationship of royalisin, an antimicrobial peptide from royal jelly of Apis mellifera

Bíliková, Katarína; Huang, Sheng-Chang; Lin, I-Ping; Šimúth, Jozef; Peng, Ching‐I

doi:10.1016/j.peptides.2015.03.001

Cited by 91 publications

(78 citation statements)

References 32 publications

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“…The major protein components of both species had different molecular weights, isoelectric points, and immunological characteristics (Takenaka & Takenaka, 1996). Many studies have shown the effect of royalisin against Gramnegative bacteria and some Gram-positive (Ilyasov et al, 2012) including the agent causing AFB (Bíliková et al, 2001;Bachanová et al, 2002;Yoshiyama & Kimura, 2010;Bílikova et al, 2015). In this study, we used royal jelly collected from A. mellifera therefore this might affect the AFB resistance of A. cerana as it may be somewhat different when compared in nature.…”

Section: Discussionmentioning

confidence: 99%

Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

Krongdang

Evans²,

Chen³

et al. 2018

Insect Science

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American foulbrood (AFB) disease is caused by Paenibacillus larvae. Currently, this pathogen is widespread in the European honey bee-Apis mellifera. However, little is known about infectivity and pathogenicity of P. larvae in the Asiatic cavity-nesting honey bees, Apis cerana. Moreover, comparative knowledge of P. larvae infectivity and pathogenicity between both honey bee species is scarce. In this study, we examined susceptibility, larval mortality, survival rate and expression of genes encoding antimicrobial peptides (AMPs) including defensin, apidaecin, abaecin, and hymenoptaecin in A. mellifera and A. cerana when infected with P. larvae. Our results showed similar effects of P. larvae on the survival rate and patterns of AMP gene expression in both honey bee species when bee larvae are infected with spores at the median lethal concentration (LC ) for A. mellifera. All AMPs of infected bee larvae showed significant upregulation compared with noninfected bee larvae in both honey bee species. However, larvae of A. cerana were more susceptible than A. mellifera when the same larval ages and spore concentration of P. larvae were used. It also appears that A. cerana showed higher levels of AMP expression than A. mellifera. This research provides the first evidence of survival rate, LC and immune response profiles of Asian honey bees, A. cerana, when infected by P. larvae in comparison with the European honey bee, A. mellifera.

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Section: Discussionmentioning

confidence: 99%

Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

Krongdang

Evans²,

Chen³

et al. 2018

Insect Science

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“…It has been reported that RJ has shown different pharmacological activities such as antimicrobial (Bílikova et al, 2015), antiviral (Hashemipour et al, 2014), antitumor (Guo et Royal jelly (RJ) is one of the most attractive bee products. RJ has long been used in traditional medicine.…”

Section: Introductionmentioning

confidence: 99%

Correlations of Physicochemical Parameters, Antioxidant Activity and Total Polyphenol Content of Fresh Royal Jelly Samples

Balkanska¹

2018

Int.J.Curr.Microbiol.App.Sci

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“…Royal jelly is a secretion of honeybee hypopharynx and mandibular salivary glands. Royalisin, which is a potent antimicrobial protein composed of 51 amino acid residues in royal jelly, has extensive sequence homology with sapecin and phormicins, both of which are composed of 40 amino acids . Nevertheless, the role of the extra stretch of 11 amino acid residues at the C‐terminus of royalisin (positions 41‐51), termed royalisin P11 (Figure ), remains unknown.…”

Section: Introductionmentioning

confidence: 99%

A fluorescently labeled undecapeptide derived from a protein in royal jelly of the honeybee—royalisin—for specific detection of oxidized low‐density lipoprotein

Sato

Unuma

Yamazaki

et al. 2018

Journal of Peptide Science

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The probes for detection of oxidized low-density lipoprotein (ox-LDL) in plasma and in atherosclerotic plaques are expected to facilitate the diagnosis, prevention, and treatment of atherosclerosis. Recently, we have reported that a heptapeptide (Lys-Trp-Tyr-Lys-Asp-Gly-Asp, KP6) coupled through the ε-amino group of N-terminal Lys to fluorescein isothiocyanate (FITC), (FITC)KP6, can be useful as a fluorescent probe for specific detection of ox-LDL. In the present study, to develop a novel fluorescent peptide for specific detection of ox-LDL, we investigated the interaction (with ox-LDL) of an undecapeptide corresponding to positions 41 to 51 of a potent antimicrobial protein (royalisin, which consists of 51 residues; from royal jelly of honeybees), conjugated at the N-terminus to FITC in the presence of 6-amino-n-caproic acid (AC) linker, (FITC-AC)-royalisin P11, which contains both sequences, Phe-Lys-Asp and Asp-Lys-Tyr, similar to Tyr-Lys-Asp in (FITC)KP6. The (FITC-AC)-royalisin P11 bound with high specificity to ox-LDL in a dose-dependent manner, through the binding to major lipid components in ox-LDL (lysophosphatidylcholine and oxidized phosphatidylcholine). In contrast, a (FITC-AC)-shuffled royalisin P11 peptide, in which sequences Phe-Lys-Asp and Asp-Lys-Tyr were modified to Lys-Phe-Asp and Asp-Tyr-Lys, respectively, hardly bound to LDL and ox-LDL. These findings strongly suggest that (FITC-AC)-royalisin P11 may be an effective fluorescent probe for specific detection of ox-LDL and that royalisin from the royal jelly of honeybees may play a role in the treatment of atherosclerosis through the specific binding of the region at positions 41 to 51 to ox-LDL.

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Structure and antimicrobial activity relationship of royalisin, an antimicrobial peptide from royal jelly of Apis mellifera

Cited by 91 publications

References 32 publications

Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

Comparative susceptibility and immune responses of Asian and European honey bees to the American foulbrood pathogen, Paenibacillus larvae

Correlations of Physicochemical Parameters, Antioxidant Activity and Total Polyphenol Content of Fresh Royal Jelly Samples

A fluorescently labeled undecapeptide derived from a protein in royal jelly of the honeybee—royalisin—for specific detection of oxidized low‐density lipoprotein

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