Structure and Assembly of the Influenza A Virus Ribonucleoprotein Complex

Abstract

Edited by Wilhelm JustKeywords: Influenza A virus Ribonucleoprotein complex RNP Nucleoprotein NP Structure Assembly a b s t r a c tThe genome of influenza A viruses consists of eight segments of single-stranded, negative-sense RNA that are encapsidated as individual rod-shaped ribonucleoprotein complexes (RNPs). Each RNP contains a viral RNA, a viral polymerase and multiple copies of the viral nucleoprotein (NP). Influenza A virus RNPs play important roles during virus infection by directing viral RNA replication and transcription, intracellular transport of the viral RNA, gene reassortment as well as viral genome packaging into progeny particles. As a unique genomic entity, the influenza A virus RNP has been extensively studied since the 1960s. Recently, exciting progress has been made in studying the RNP structure and its assembly, leading to a better understanding of the structural basis of various RNP functions.

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“…Genomes of influenza A and B viruses are structurally similar, and consist of eight RNA genome segments of negative polarity. These genome segments encode 12 proteins and are named according to the product translated from the primary open reading frame: PB1, PB2, PA, HA, NP, NA, M, and NS [6]. The polymerase complex PB2, PB1, and PA transcribes one mRNA from each genomic fragment, which is translated to the corresponding protein.…”

Проблема Создания Универсальной Противогриппозной Вакцины

“…Геномы вирусов грип-па А и В структурно схожи, они состоят из 8 геном-ных сегментов РНК негативной полярности, которые кодируют 12 белков и названы по продукту, транс-лируемому с основной открытой рамки считывания: PB1, PB2, PA, HA, NP, NA, M и NS [6]. Полимеразный комплекс PB2, PB1, PA транскрибирует одну мРНК с каждого геномного фрагмента, транслируемую в одноименный белок.…”

Quantum dots crack the influenza uncoating puzzle

“…A single NP molecule binds 24 to 27 nucleotides in vivo (6, 7) and has two sets of nuclear localization signals (NLSs), ensuring robust nuclear import of uncoated vRNPs during entry (8,9). vRNPs are 10 nm in width and 30 to 110 nm in length (10) and package themselves into a revolving cylinderlike orientation, adopting a typical 7+1 arrangement with seven vRNPs surrounding a central one (11,12). All eight viral RNA segments contain unique packaging sequences residing mainly in the 3′ and 5′ termini of each viral RNA (13), ensuring the packaging of eight distinct vRNPs into budding particles.…”