Structure and characterization of the human insulin-like growth factor binding protein (IGFBP)-6 promoter: identification of a functional retinoid response element

Dailly, Yves P.; Zhou, Yue; Linkhart, Thomas A.; Baylink, David J.; Strong, Donna D.

doi:10.1016/s0167-4781(01)00192-0

Cited by 18 publications

(11 citation statements)

References 34 publications

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“…In line with this, compounds stimulating or inducing differentiation in these cell lines such as retinoic acid (RA), vitamin D, steroid hormones, cAMP, and several growth factors including IGF-1 upregulate IGFBP-6 expression (Bach et al, 1998;Chambery et al, 1998Chambery et al, , 2000Bach, 1999;Dailly et al, 2001). In the O2A assays, IGF-1 and insulin only slightly increased IG-FBP-6 mRNA.…”

Section: Discussionmentioning

confidence: 87%

Insulin‐like growth factor‐binding protein 6 inhibits survival and differentiation of rat oligodendrocyte precursor cells

Kühl

Hoekstra

Vries

et al. 2003

Glia

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Insulin-like growth factor 1 (IGF-1) is a growth and survival factor for oligodendrocyte lineage cells and promotes myelination. We demonstrate that IGF-binding protein 6 (IGFBP-6) is expressed and localized to the Golgi complex in rat oligodendrocyte precursor (O2A) cells. IGFBP-6 mRNA showed a developmentally regulated expression pattern, displaying a transient decrease during early development, and enhanced levels upon cell maturation. IGFBP-6 mRNA expression could be reduced by addition of basic fibroblast growth factor and progesterone while estrogen increased IGFBP-6 mRNA. IGF-1, platelet-derived growth factor, and insulin had no effect. When added exogenously, IGFBP-6 reduced O2A cell survival in the absence of IGF-1 and inhibited IGF-1-stimulated survival in a partially IGF-1-dependent and partially IGF-1-independent fashion. In addition, IGFBP-6 reduced the IGF-stimulated expression of two myelin proteins, CNPase and MAG. Taken together, the data show that IGFBP-6 is a new negative effector of oligodendrocyte survival and differentiation.

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Section: Discussionmentioning

confidence: 87%

Insulin‐like growth factor‐binding protein 6 inhibits survival and differentiation of rat oligodendrocyte precursor cells

Kühl

Hoekstra

Vries

et al. 2003

Glia

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“…Our results confirm that IGFBP-6 is an estradiol-responsive gene. Dailly (Dailly et al, 2001) analyzed the promoter activity of the IGFBP-6 gene 5' -flanking region in the SaOS-2 and U-2OS cell lines. Deletion analysis showed the promoted activity of IGFBP-6 gene requires the -1,731 to +154 bp sequence of the gene promoter, and the important elements include a putative RARE, an AP-2 site, a CACCC box, and an Sp1 site (Dailly et al, 2001;Song et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Dailly (Dailly et al, 2001) analyzed the promoter activity of the IGFBP-6 gene 5' -flanking region in the SaOS-2 and U-2OS cell lines. Deletion analysis showed the promoted activity of IGFBP-6 gene requires the -1,731 to +154 bp sequence of the gene promoter, and the important elements include a putative RARE, an AP-2 site, a CACCC box, and an Sp1 site (Dailly et al, 2001;Song et al, 2001). We found that estrogen activity in SaOS-2 cells is primarily associated with the -44 to +118 region of the IGFBP-6 gene promoter that contains the liganded-ER binding site in the -9 to +1 region (Figure 2 and 4).…”

Section: Discussionmentioning

confidence: 99%

“…Structural and functional analyses of the IGFBP-6 gene promoter have been performed (Hodzic et al, 1997;Dailly et al, 2001), and the 5'-promoter region contains retinoic acid response elements, CAAT boxes, CACCC boxes, NF-1, and activated protein-1 (AP-1)/AP-2 sites (Dailly et al, 2001;Song et al, 2001). However, the molecular mechanism of estrogen signaling through ER in IGFBP-6 transcription has not been determined.…”

Section: Figurementioning

confidence: 99%

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Transcriptional activation of insulin-like growth factor binding protein 6 by 17β-estradiol in SaOS-2 cells

et al. 2009

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Osteoblasts can synthesize the insulin-like growth factors (IGFs) and the IGF-binding proteins (IGFBPs), which may either enhance or attenuate IGF-stimulated bone cell proliferation. Since estrogen induced osteoblastic differentiation and proliferation through an estrogen-responsive gene in target cells, we investigated the effects of estrogen on IGFBP-6 expression in the human osteoblastic-like cell line SaOS-2. Expressions of IGFBP-6 protein and mRNA increased 2.8 and 2-fold, respectively, in the presence of 17-β-estradiol (E2) (0.01 to 1 μM) and estrogen receptor (ER) in SaOS-2 cells. On the other hand, E2 induced a 2-fold increase in SaOS-2 cell proliferation. To identify genomic sequences associated with estrogen responsiveness, the 5'-promoter region (-44 to +118) of the IGFBP-6 gene was cloned into a chloramphenicol acetyltransferase (CAT) reporter vector. E2 induced a 3-fold increase in CAT activity in SaOS-2 cells transiently transfected with this construct. Identification of the estrogen-responsive element (ERE) [5'-CCTTCA CCTG-3'] (-9 to +1) in this IGFBP-6 gene promoter region was confirmed using electromobility shift assays and deletion analysis. This functional ERE was important for E2-induced trans-activation of the IGFBP-6 gene.These results demonstrate that E2 exhibits a positive effect on IGFBP-6 gene transcription through estrogen-liganded ER binding to the functional ERE in the IGFBP-6 gene promoter in SaOS-2 cells.

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“…HCA 3 cells were treated with a variety of chemicals, including a DNA-damaging agent (cis-platin), DNA polymerase inhibitor (hydroxyurea), microtubule disruptor (colchicine), amino acid analogue (mimosine), mitochondrial uncoupler (rhodamine), and reducing agents (dithiothreitol and N-ethylmaleimide). The nuclear receptor agonist retinoic acid, which has been reported to induce IGFBP-6 expression (38,39), was included as a comparison. Cells were treated with a sublethal dose of toxins and were allowed to recover for 3 days before collecting the conditioned medium and RNA as described under "Materials and Methods."…”

Section: Fig 2-continuedmentioning

confidence: 99%

Proteomic Identification of Insulin-like Growth Factor-binding Protein-6 Induced by Sublethal H2O2 Stress from Human Diploid Fibroblasts

Xie

Tsaprailis²,

Chen

2005

Molecular & Cellular Proteomics

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Fibroblasts are the most ubiquitous cell types within our body. They produce various factors to maintain the texture and structure of a particular organ or tissue. To identify protein factors secreted by fibroblasts and alteration of these protein factors upon oxidative stress, HCA 3 human skin diploid fibroblasts were exposed to a sublethal dose of H 2 O 2 , which induces a prematurely senescent phenotype. Conditioned media from prematurely senescent cells versus control cells were analyzed for proteins using an LC-MS/MS-based proteomic technique. Collagen ␣1(VI), collagen ␣2(I), fibronectin, lumican, and matrix metalloproteinase 2 were among the proteins consistently detected from control and H

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Structure and characterization of the human insulin-like growth factor binding protein (IGFBP)-6 promoter: identification of a functional retinoid response element

Cited by 18 publications

References 34 publications

Insulin‐like growth factor‐binding protein 6 inhibits survival and differentiation of rat oligodendrocyte precursor cells

Insulin‐like growth factor‐binding protein 6 inhibits survival and differentiation of rat oligodendrocyte precursor cells

Transcriptional activation of insulin-like growth factor binding protein 6 by 17β-estradiol in SaOS-2 cells

Proteomic Identification of Insulin-like Growth Factor-binding Protein-6 Induced by Sublethal H2O2 Stress from Human Diploid Fibroblasts

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