Structure and component alteration of rabbit Achilles tendon in tissue culture

Hosaka, Yoshinao Z.; Yamasaki, Tadatsugu; Suzuki, Daisuke; Matsuda, Noboru; Takehana, Kazushige

doi:10.2220/biomedres.26.279

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…TNFα is produced by various cells and enhances many biological events, including production of MMPs and stimulation or inhibition of cellular proliferation, and secreted TNFα stimulates cells to synthesize IL-1β in a para/autocrine manner (12,27,28). Even in the tendon, these gelatinases and pro-infl ammatory cytokines are synthesized by tendinocytes and are involved in the turnover of tendon tissue and in the maintenance of homeostasis (9,15,16,23). In several types of fi broblast-like cells, pro-infl ammatory cytokines can activate both MMP-2 and -9 (45).…”

Section: Figmentioning

confidence: 99%

Effect of heat on synthesis of gelatinases and pro-inflammatory cytokines in equine tendinocytes

et al. 2006

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The aim of this study was to clarify whether matrix metalloproteinases (MMP-2 and -9: gelatinases) and pro-infl ammatory cytokines [tumor necrosis factor (TNF) α and interleukin (IL)-1β] are induced by heat in tendon tissue in vitro and to test the hypothesis that heat exposure causes tendinocytes to synthesize pro-infl ammatory cytokines and that synthesis of these cytokines, in turn, leads to up-regulation of synthesis of gelatinases. Isolated tendinocytes from equine superfi cial digital fl exor tendons were cultured and all experiments were performed on cells passaged 3 or 4 times. In the cells exposed to heat (37 to 45°C, 0 to 60 min), the survival rate decreased sharply in a temperature-and time-dependent manner, especially at 42 and 45°C. Cells exposed at 40°C, however, showed little change in survival rate and morphology. Gelatin zymograms revealed that proMMP-2 and -9 were the only two MMPs remaining in the supernatant of the cultured tendinocytes, including that of untreated cells. Addition of TNFα and IL-1β to the culture medium of tendinocytes accelerated proMMP-9 synthesis considerably. Heating the tendinocytes (40°C) led to a three-fold increase in proMMP-9 synthesis in a short time. Only TNFα was detected in tendinocytes after heat exposure for 30 and 60 min. In contrast, IL-1β was under the detectable level in ELISA. Cooling of heat-exposed cells from 40°C to 37°C considerably down-regulated cellular proMMP-9 synthesis. Furthermore, proMMP-9 level was greatly reduced in cells treated at lower temperatures, 20°C and 5°C. These fi ndings support our hypothesis that hyperthermia in the horse tendon induces tendinocytes to synthesize pro-infl ammatory cytokines and that the synthesis of these cytokines results in the up-regulation of gelatinases.Tendon injury, especially in the superfi cial digital flexor tendon (SDFT), has proved to be a major problem for racehorses. Recent studies have shown that about 10-30% of racehorses suffer from tendonitis (29). Although the etiology of tendonitis has been discussed in many reports (2,19,25), there is little information on the mechanism of tendon degradation. Pro-infl ammatory cytokines, such as tumor necrosis factor (TNF) α and interleukin (IL)-1β, and gelatinases in matrix metalloproteinases (MMPs) are involved in degradation of connective tissues (30,33,34,40), and these pro-infl ammatory cytokines and gelatinases are synthesized in tendinocytes (15,18,33). Additionally, TNF α and IL-1β are known to be initiators of the synthesis of gelatinases and of the synthesis and release of cytokines (3,7,8,10,24,28).

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Section: Figmentioning

confidence: 99%

Effect of heat on synthesis of gelatinases and pro-inflammatory cytokines in equine tendinocytes

et al. 2006

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“…The tumor necrosis factor receptor superfamily (TNFRSF) is an ancient family of molecules which is present in both vertebrates (Su et al, 1992; Tan et al, 1997; Bridgham et al, 2001; Bridgham and Johnson, 2003; Tamura et al, 2004; Hosaka et al, 2005; Iha et al, 2009) and invertebrates (Ouwe-Missi-Oukem-Boyer et al, 1994; Kauppila et al, 2003; Wang et al, 2006; Kato et al, 2009; Li et al, 2009). For many years, the focus on the immunological and pathological role of TNFRSF members and their ligands (the TNFSF or tumor necrosis factor superfamily) has overshadowed research examining non-immunological functions for these proteins.…”

Section: Introductionmentioning

confidence: 99%

The role of tumor necrosis factor receptor superfamily members in mammalian brain development, function and homeostasis

Twohig

Cuff

Yong

et al. 2011

Reviews in the Neurosciences

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Tumor necrosis factor receptor superfamily (TNFRSF) members were initially identified as immunological mediators, and are still commonly perceived as immunological molecules. However, our understanding of the diversity of TNFRSF members' roles in mammalian physiology has grown significantly since the first discovery of TNFRp55 (TNFRSF1) in 1975. In particular, the last decade has provided evidence for important roles in brain development, function and the emergent field of neuronal homeostasis. Recent evidence suggests that TNFRSF members are expressed in an overlapping regulated pattern during neuronal development, participating in the regulation of neuronal expansion, growth, differentiation and regional pattern development. This review examines evidence for non-immunological roles of TNFRSF members in brain development, function and maintenance under normal physiological conditions. In addition, several aspects of brain function during inflammation will also be described, when illuminating and relevant to the non-immunological role of TNFRSF members. Finally, key questions in the field will be outlined.

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“…This was associated with histological evidence of collagen degradation. Other studies have reported an increase in MMP2 (Hosaka et al, 2005), MMP9 (Hosaka et al, 2005; Jones et al, 2006), and MMP13 (Nomura et al, 2007) expression in models of chronic tendonitis.…”

Section: Discussionmentioning

confidence: 85%

The effect of tissue culture on suture holding strength and degradation in canine tendon

Omae

Zhao

Sun

et al. 2009

J Hand Surg Eur Vol

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The purpose of this study was to assess tendon metabolism and suture pull-out strength after simple tendon suture in a tissue culture model. One hundred and twelve flexor digitorum profundus tendons from 28 dogs were cultured for 7, 14, or 21 days with or without a static tensile load. In both groups increased levels of matrix metalloproteinase (MMP) mRNA was noted. Suture pull-out strength did not decrease during tissue culture. While the presence of a static load had no effect on the pull-out strength, it did affect MMP mRNA expression. This tissue culture model could be useful in studying the effect of factors on the tendon-suture interface.

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Structure and component alteration of rabbit Achilles tendon in tissue culture

Cited by 6 publications

References 35 publications

Effect of heat on synthesis of gelatinases and pro-inflammatory cytokines in equine tendinocytes

Effect of heat on synthesis of gelatinases and pro-inflammatory cytokines in equine tendinocytes

The role of tumor necrosis factor receptor superfamily members in mammalian brain development, function and homeostasis

The effect of tissue culture on suture holding strength and degradation in canine tendon

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