Structure and expression of a cloned cDNA for human interleukin-2

Abstract

A cDNA coding for human interleukin-2 (IL-2) has been cloned from a cDNA library prepared from partially purified IL-2 mRNA. The DNA sequence codes for a polypeptide which consists of 153 amino acids including a putative signal sequence. A biologically active polypeptide, characteristic of human IL-2, was produced when the cDNA was fused to a simian virus 40 promoter sequence and used to transfect cultured monkey COS cells.

Small-Molecule Inhibitors of IL-2/IL-2R: Lessons Learned and Applied

“…The gene for IL-2 was cloned in 1983 (Degrave et al 1983;Taniguchi et al 1983), and the crystal structure ( Fig. 3) determined in 1987 (Brandhuber et al 1987).…”

Le renouveau de l’interleukine 2

“…En retour, la prolifération de ces lignées ou de ces clones T a servi de révélateur pour permettre la purification et la caracté-risation biochimique de la cytokine. L'IL-2 a aussi été la première cytokine à être clonée, en 1983 [2].…”

Expression and purification of human IL-2 protein from Escherichia coli

“…After recombinant protein technology, IL-2 was expressed after isolation of IL-2 mRNA from human leukemic T-cell line, followed by cloning of the complementary cDNA into a suitable vector. The cloned IL-2 was expressed in different hosts such as E. coli (Ju et al, 1987;Devos et al, 1983;Rosenberg et al, 1984), insect cells (Smith et al, 1985), and monkey COS cells (Taniguchi et al, 1983). Most of the expressed IL-2 in E. coli was reported to be insoluble (forms IBs), however, the recombinant IL-2 was still retaining its biological activity even after extraction from SDS polyacrylamide gel (Devos et al, 1983).…”