Structure and expression of a chicken gene coding for U1 RNA

Roop, Dennis R.; Kristo, Paula; Stumph, William E.; Tsai, Ming‐Jer; O’Malley, Bert W.

doi:10.1016/0092-8674(81)90430-x

Cited by 132 publications

(93 citation statements)

References 51 publications

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“…In birds and mammals the genes are not present in an obvious repeating unit (1)(2)(3), although the possibility of a large repeated unit cannot be ruled out for humans since the genes isolated thus far are virtually identical in both the 5' and 3' flanking regions (4). The human U2 genes are organized in a large repeated unit (7).…”

Section: Introductionmentioning

confidence: 99%

“…Multiple copies of the Ul RNA gene are present in all organisms in which these genes have been studied (1)(2)(3)(4)(5)(6). In birds and mammals the genes are not present in an obvious repeating unit (1)(2)(3), although the possibility of a large repeated unit cannot be ruled out for humans since the genes isolated thus far are virtually identical in both the 5' and 3' flanking regions (4).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structure and expression of a second sea urchin U1 RNA gene repeat

Nash

Santiago

et al. 1986

Nucl Acids Res

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure and expression of a second sea urchin U1 RNA gene repeat

Nash

Santiago

et al. 1986

Nucl Acids Res

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“…So far most studies of snRNA genes have concerned pseudogenes, many of which appear to have widely different flanking sequences (Westin et al, 1981). Roop et al (1981), however, studied the distribution of U I sequences in the chicken genome and came to the conclusion that 6-10 copies are present per haploid genome equivalent. Sequences analysis of one chicken clone revealed a structure that perfectly matches the sequence of chicken Ul RNA (Roop et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

“…Roop et al (1981), however, studied the distribution of U I sequences in the chicken genome and came to the conclusion that 6-10 copies are present per haploid genome equivalent. Sequences analysis of one chicken clone revealed a structure that perfectly matches the sequence of chicken Ul RNA (Roop et al, 1981). This observation poses the question whether the wide distribution of pseudogenes is a feature specific for higher eukaryotes since the pseudogenes appear to predominate both in the mouse and the human genome.…”

Section: Discussionmentioning

confidence: 99%

A candidate gene for human U1 RNA

1982

The EMBO Journal

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Clones containing sequences complementary to the small nuclear RNA Ul were isolated from the human DNA library of Lawn et al. (1978). Three clones were studied by hybrdization and restriction enzyme cleavage. The results showed that the inserts in all three clones were different and that each clone contains one single copy of a sequence which hybridizes to Ul RNA. The results revealed moreover that only one of the three clones contains all the cleavage sites which can be predicted from the known sequence of human U1 RNA, suggesting that the three clones comprise one candidate Ul gene and two pseudogenes. A fragment from the recombinant with the candidate Ul gene was subcloned in the pPR322 plasmid and part of its sequence was determined. The results showed that the subclone contains a sequence which matches that of the human Ul RNA perfectly. The sequence "TATAT" which often is found adjacent to RNA polymerase U start sites, was identified 33-37 base pairs upstream from the beginning of the Ul sequence. Two ten base pairs long, nearly perfect, direct repeats were also identified in the vicinity of the Ul sequence and an imperfect inverted repeat follows immediately after the Ul gene.

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“…The estimates for gene copy number range from ca. 5 to 50 per haploid genome in Drosophila (S. M. Mount, Ph.D. thesis, Yale University, New Haven, Conn., 1983) sea urchin (5), chicken (24), rodent (16,30), and human (9,14,15) DNAs. In spite of this multiplicity of genes, only one or two Ul RNA species have been detected in each of these organisms.…”

mentioning

confidence: 99%