Structure and function of an archetypal respiratory chain complex: NADH-ubiquinone reductase

Ci, Ragan

doi:10.1042/bst0180515

Cited by 22 publications

(12 citation statements)

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“…Briefly, the activity of mitochondrial complex I was measured according to the method described by Ragan [32], which follows the decrease in NADH absorbance (340 nm) that occurs when ubiquinone (CoQ 1 ) is reduced to form ubiquinol. The activity of mitochondrial complex I was expressed as 1 nmol of NADH oxidized per minute per milligram of mitochondrial protein.…”

Section: Methodsmentioning

confidence: 99%

Effects of dietary leucine supplementation on the hepatic mitochondrial biogenesis and energy metabolism in normal birth weight and intrauterine growth-retarded weanling piglets

Zhang

et al. 2017

Nutr Res Pract

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BACKGROUND/OBJECTIVESThe study was conducted to evaluate the effects of dietary leucine supplementation on mitochondrial biogenesis and energy metabolism in the liver of normal birth weight (NBW) and intrauterine growth-retarded (IUGR) weanling piglets.MATERIALS/METHODSA total of sixteen pairs of NBW and IUGR piglets from sixteen sows were selected according to their birth weight. At postnatal day 14, all piglets were weaned and fed either a control diet or a leucine-supplemented diet for 21 d. Thereafter, a 2 × 2 factorial experimental design was used. Each treatment consisted of eight replications with one piglet per replication.RESULTSCompared with NBW piglets, IUGR piglets had a decreased (P < 0.05) hepatic adenosine triphosphate (ATP) content. Also, IUGR piglets exhibited reductions (P < 0.05) in the activities of hepatic mitochondrial pyruvate dehydrogenase (PDH), citrate synthase (CS), α-ketoglutarate dehydrogenase (α-KGDH), malate dehydrogenase (MDH), and complexes I and V, along with decreases (P < 0.05) in the concentration of mitochondrial DNA (mtDNA) and the protein expression of hepatic peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α). Dietary leucine supplementation increased (P < 0.05) the content of ATP, and the activities of CS, α-KGDH, MDH, and complex V in the liver of piglets. Furthermore, compared to those fed a control diet, piglets given a leucine-supplemented diet exhibited increases (P < 0.05) in the mtDNA content and in the mRNA expressions of sirtuin 1, PGC-1α, nuclear respiratory factor 1, mitochondrial transcription factor A, and ATP synthase, H+ transporting, mitochondrial F1 complex, β polypeptide in liver.CONCLUSIONSDietary leucine supplementation may exert beneficial effects on mitochondrial biogenesis and energy metabolism in NBW and IUGR weanling piglets.

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Section: Methodsmentioning

confidence: 99%

Effects of dietary leucine supplementation on the hepatic mitochondrial biogenesis and energy metabolism in normal birth weight and intrauterine growth-retarded weanling piglets

Zhang

et al. 2017

Nutr Res Pract

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“…Citrate synthase activity was determined by the method used by Haas et al (30). Mitochondria complex I activity was determined by the method used by Ragan (31). Mitochondria complex II activity was measured based on the method of Sottocasa et al (32).…”

Section: Methodsmentioning

confidence: 99%

Dietary Olive and Perilla Oils Affect Liver Mitochondrial DNA Methylation in Large Yellow Croakers

Liao

Mai

et al. 2015

The Journal of Nutrition

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“…It is known that electron transfer through complex I is coupled to proton translocation and the consensus opinion is that the H + /2e − ratio is 4 [35,36]. However, the lack of structural and functional data has hampered understanding of the coupling mechanism.…”

Section: Complex Imentioning

confidence: 98%

The molecular machinery of Keilin's respiratory chain

Rich

2003

Biochemical Society Transactions

334

207

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Keilin's classic paper of 1925 [Keilin (1925) Proc. R. Soc. London Ser. B 100, 129-151], achieved with simple, but elegant, techniques, describes the cytochrome components of the respiratory chain and their roles in intracellular respiration and oxygen consumption. Since that time, a tremendous amount of work has clarified the intricate details of the prosthetic groups, cofactors and proteins that comprise the respiratory chain and associated machinery for ATP synthesis. The work has culminated in advanced crystallographic and spectroscopic methods that provide structural and mechanistic details of this mitochondrial molecular machinery, in many instances to atomic level. I review here the current state of understanding of the mitochondrial respiratory chain in terms of structures and dynamics of the component proteins and their roles in the biological electron and proton transfer processes that result in ATP synthesis. These advances, together with emerging evidence of further diverse roles of mitochondria in health and disease, have prompted a new era of interest in mitochondrial function.

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Structure and function of an archetypal respiratory chain complex: NADH-ubiquinone reductase

Cited by 22 publications

References 0 publications

Effects of dietary leucine supplementation on the hepatic mitochondrial biogenesis and energy metabolism in normal birth weight and intrauterine growth-retarded weanling piglets

Effects of dietary leucine supplementation on the hepatic mitochondrial biogenesis and energy metabolism in normal birth weight and intrauterine growth-retarded weanling piglets

Dietary Olive and Perilla Oils Affect Liver Mitochondrial DNA Methylation in Large Yellow Croakers

The molecular machinery of Keilin's respiratory chain

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