Structure and function of the cytoskeleton in filamentous fungi

Raudaskoski, Marjatta; Salo, Vanamo; Niini, Sara S.

doi:10.29203/ka.1988.265

Cited by 25 publications

(4 citation statements)

References 31 publications

(49 reference statements)

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“…In the S. bovinus-P. sylvestris ectomycorrhiza tbe microtubule tracks beneatb the plasma membrane in mantle bypbae are comparable to tbose known from non-symbiotic byphae (Salo et al, 1989), altbougb tbe polarity of microtubule tracks towards tbe byphal tips was difficult to recognize in tbe mantle bypbae. In tbe non-symbiotic bypbae tbe microtubule tracks are involved in organelle movement and distribution associated with the extension growth and branching of the hypbae (Raudaskoski et al, 1988), which is also needed in tbe mantle for its development in concert witb the elongation of tbe sbort root. Tbe frequent occurrence of dividing nuclei in tbe bypbae in tbe mantle around the root apex indicate active growth.…”

Section: Resultsmentioning

confidence: 99%

Identification of cytoskeletal components in pine ectomycorrhizas

et al. 1993

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SUMMARYEctomycorrhizal associations were synthesized befween pine seedlings and the fungi Suillus bovinus (L.) ex Fr. or Paxillus involutus (Batsch ex Fr.) Fr. Immunoblotting of polypeptides separated electrophoretically from crude tissue extracts revealed the abundant presence of tubulin and aetin in ectomycorrhiza and lower amounts in the fungal strands surrounding the ectomycorrhizal roots. In ectomycorrhiza the a-tubulins from fungal hyphae and plant cells were clearly distinguishable but such discrimination was not possible for /^-tubulin or aetin due to the similar mobility of proteins originating from the conifer and fungal tissues. Young ectomycorrhizal short roots were fixed while still attached to the seedlings and, using indirect immunofluorescence microscopy with tubulin antibodies, microtubules were detected in both the conifer cells and in fungal hyphae. In the host plant cytoplasmic and spindle microtubules were visualized in meristem cells and in differentiating vascular tissue but not in the cortical cells. In symbiotic hyphae the microtubule tracks and spindles of dividing nuclei were clearly distinguished in the mantle hyphae in the tip region of the short roots. In the Hartig net hyphae microtubule tracks changed to a less clear, reticulate structure. Aetin was visualized as long filaments in vascular tissue cells and as small microfilament bundles in mantle hyphae. Short microtubules and aetin dots were detected in cytoplasmcontaining hyphae on the strand surface. The possible role of the cytoskeletal elements in the maintenance of the ectomycorrhizal association is discussed.

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Section: Resultsmentioning

confidence: 99%

Identification of cytoskeletal components in pine ectomycorrhizas

et al. 1993

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“…Fungi contain actin, but its diverse functions are not well understood [1,2]. Evidence accu-Correspondence to: F. Barja, Laboratory of General Microbiology, University of Geneva, Sciences III, 30, Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.…”

Section: Introductionmentioning

confidence: 99%

Localization of actin and characterization of its isoforms in the hyphae of Neurospora crassa

Barja

1991

FEMS Microbiology Letters

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The actin of Neurospora crassa wild type Strain St. Lawrence has been purified, characterized and localized. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I-Sepharose. This protein was identified as actin on immunoblots when an anti-actin monoclonal antibody raised against chicken gizzards was used as a probe. After two-dimensional gel electrophoresis three actin isoforms were detected. The distribution of actin in hyphae was examined by FITC-phalloidin staining of formaldehyde fixed hyphae. F-actin was found to be mainly concentrated in the hyphal tips in which it formed a uniform cap. Apical actin could be involved in hyphal morphogenesis, organelle motility and maintenance of polarity.

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“…Microtubules have been observed in many fungi [1] such as in the aquatic moulds of the genus Allomyces in which they were found to be present in the subapical zone of the vegetative hyphae [2] and in the zoospores [3]. They are known to be involved in flagellar motility [4][5][6], chromosome segregation [7], maintenance of cellular morphology [8] and intracellular transport of organelles including mitochondria and vesicles Correspondence to: I. Sch6nenberger, Laboratory of General Microbiology, University of Geneva, Sciences III, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.…”

Section: Introductionmentioning

confidence: 99%

Evolution of tubulin isoforms during the sporophytic development of Allomyces arbuscula

SCHONENBERGER

1993

FEMS Microbiology Letters

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Tubulins extracted from the sporophytic developmental stages of Allomyces arbuscula have been characterised by one‐ and two‐dimensional SDS‐PAGE gels immunoblotted with monoclonal antibodies as α‐, acetylated α‐ (Mr 57 kDa both) and β‐ (Mr 55 kDa) isoforms. The zoosporangial isoforms could be characterised only when precautions were taken to inhibit the strong tubulin proteolytic activity at this stage. The zoospores and zoosporangia contained greater amounts of the acetylated α‐ and β‐isoforms than the mycelium, while the non acetylated α‐isoform was present in greater quantity in the mycelium than in the zoospores or zoosporangia.

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Structure and function of the cytoskeleton in filamentous fungi

Cited by 25 publications

References 31 publications

Identification of cytoskeletal components in pine ectomycorrhizas

Identification of cytoskeletal components in pine ectomycorrhizas

Localization of actin and characterization of its isoforms in the hyphae of Neurospora crassa

Evolution of tubulin isoforms during the sporophytic development of Allomyces arbuscula

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