Structure and Functional Analysis of the Microbial Community in an Aerobic: Anaerobic Sequencing Batch Reactor (SBR) with no Phosphorus Removal

Kong, Yun; Beer, Michael; Seviour, Robert J.; Lindrea, K.; Rees, Gavin N.

doi:10.1078/0723-2020-00075

Cited by 49 publications

(71 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These isolates have been shown phylogenetically to be members of the Alphaproteobacteria (Amaricoccus spp., and 'Defluvicoccus vanus'), Betaproteobacteria (Quadracoccus sp. ), Gammaproteobacteria, and Actinobacteria (Tetrasphaera spp., Micropruina glycogenica and Kineosphaera limosa) (Maszenan et al, 1997;Kong et al, 2001;Shintani et al, 2000;Hanada et al, 2002;Liu et al, 2002a). However, except for M. glycogenica, none of those isolates have been seen in abundance in either fullor laboratory-scale EBPR processes (Kong et al, 2001;Seviour et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic–aerobic activated sludge processes

et al. 2004

View full text Add to dashboard Cite

In an acetate-fed anaerobic-aerobic membrane bioreactor, a deteriorated enhanced biological phosphorus removal (EBPR) community was developed (as determined based on the chemical profiles of organic substrate, soluble phosphate, and intracellular carbohydrate and polyhydroxyalkanote (PHA) concentrations). Microscopic observations revealed the dominance of tetrad-forming organisms (TFOs), of which the majority stained positively for PHA under anaerobic conditions. Fluorescence in situ hybridization (FISH) confirmed that the Alphaproteobacteria (85?0±7?0 % of total cells) were the most dominant group. A 16S rRNA gene clone library specific for the Alphaproteobacteria indicated that most 16S rRNA gene clones (61 % of total clones) were closely affiliated with 'Defluvicoccus vanus', forming a cluster within subgroup 1 of the Alphaproteobacteria. Combined PHA staining and FISH with specific probes designed for the members of the 'Defluvicoccus' cluster suggested diversity within this TFO cluster, and that these TFOs were newly identified glycogen-accumulating organisms in EBPR systems. However, these 'Defluvicoccus'-related TFOs were only seen in low abundance in 12 different EBPR and non-EBPR systems, suggesting that they were not the key populations responsible for the deterioration of full-scale EBPR processes.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic–aerobic activated sludge processes

et al. 2004

View full text Add to dashboard Cite

show abstract

“…FISH combined with Neisser staining for polyphosphate and Nile blue staining for polyhydroxyalkanoates (PHAs) were carried out following the procedures described by Kong et al (2004Kong et al ( , 2006. FISH combined with 49,6-diamidino-2-phenylyindole (DAPI) staining was carried out as described previously (Kong et al, 2001). …”

mentioning

confidence: 99%

Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant

et al. 2007

View full text Add to dashboard Cite

The structure and function of the microbial community in a full-scale enhanced biological phosphorus removal wastewater treatment plant (WWTP; Skagen) were investigated using the full-cycle rRNA approach, combined with ecophysiological studies. A total of 87 16S rRNA gene sequences were retrieved, and 78 operational taxonomic units were identified. Novel oligonucleotide probes were designed, and quantitative fluorescence in situ hybridization revealed that six hitherto undescribed probe-defined groups within the phylum Bacteroidetes (two groups), and classes Betaproteobacteria (two groups) and Gammaproteobacteria (two groups), were relatively abundant (.1 % of total biovolume) in the Skagen WWTP and 10 other full-scale WWTPs with biological P removal. The most abundant was a group of rod-shaped Bacteroidetes attached to filamentous bacteria, which is distantly related to the genus Haliscomenobacter of the family Saprospiraceae, and comprised 9-19 % of the bacterial biovolume in all the WWTPs investigated. The other five probe-defined groups were found in all WWTPs, but they were less abundant (1-6 %). Two groups had a glycogen-accumulating phenotype and one Dechloromonas-related group had a polyphosphate-accumulating phenotype, and they were potentially all involved in denitrification. In total, about 81 % of all bacteria hybridizing with the general eubacterial probe were detected in the Skagen WWTP by using clone-or group-specific probes, indicating that most members of the microbial community had been identified. INTRODUCTIONAn increasing number of wastewater treatment plants (WWTPs) are designed, or have been upgraded, to remove C, N and P by using microbial activity in the process known as enhanced biological phosphorus removal (EBPR). This process is popular mainly because it is more environmentally friendly, due to reduced use of chemicals and low sludge production, compared to P removal by chemical precipitation (Seviour et al., 2003). The general understanding of design and operation of activated-sludge WWTPs has increased significantly in the last two decades, but occasionally EBPR WWTPs still suffer from suboptimal operation and breakdown of the P-removal process. These problems are believed to be due to poor understanding of the structure of the microbial community in EBPR WWTPs and insufficient knowledge of the ecophysiology of the key microbial populations.Our understanding of the microbiology of EBPR WWTPs is to a large extent obtained from culture-independent studies using enriched cultures in well-controlled laboratory-scale reactors by using the full-cycle rRNA approach (Amann, 1995), as reviewed in detail by Mino et al. (1998) and Seviour et al. (2003). Some potentially important micro-organisms involved in the EBPR process have been identified in such laboratory-scale reactors, but only a few of them have been shown to be important in full-scale WWTPs. These are all uncultured organisms and can thus only be detected and quantified by using molecular methods such as fluorescence in situ hybrid...

show abstract

“…The performance of EBPR systems depends on the competition of PAOs with GAOs, so it is desirable to minimise the growth of the latter in reactors [33]. Most research is carried out in lab-scale SBRs fed with a single carbon source, usually acetate or propionate [10,12,[27][28][29][30][31][32]49]. Due to the nature of these experiments conducted with highly enriched cultures, key aspects of PAO and GAO ecophysiology and their competition in full-scale systems need elucidation.…”

Section: Wastewater Compositionmentioning

confidence: 99%

“…In the majority of lab-scale experiments, SBRs with 6 h cycles were used to enrich either PAOs or GAOs [2,11,12,15,27,28,[30][31][32][33]49,53].…”

Section: Sbr Configuration and Lab-scale Conditionsmentioning

confidence: 99%

From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor

et al. 2013

View full text Add to dashboard Cite

A laboratory scale sequencing batch reactor (SBR), fed with synthetic wastewater containing a mixture of organic compounds, was operated for nearly six months. Despite maintaining the same operational conditions, a deterioration of enhanced biological phosphorus removal (EBPR) occurred after 40 days of SBR operation. The Prel/Cupt ratio decreased from 0.28 to 0.06 P-mol C-mol−1, and C requirements increased from 11 to 32 mg C h−1 g−1 of mixed liquor suspended solids. A FISH analysis showed that the percentage of Accumulibacter in an overall community of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) dropped from 93% to 13%. An increase in abundance of Gammaproteobacteria (from 2.6% to 22%) and Alphaproteobacteria (from 1.8% to 10%) was observed. The number of Competibacter increased from 0.5% to nearly 9%. Clusters 1 and 2 of Defluviicoccus-related GAOs, not detected before deterioration, constituted 35% and 27% of Alphaproteobacteria, respectively. We concluded that lab-scale experiments should not be extended implicitly to full-scale EBPR systems because some bacterial groups are detected mainlyin lab-scale reactors. Well-defined, lab-scale operational conditions reduce the number of ecological niches available to bacteria.

show abstract

Structure and Functional Analysis of the Microbial Community in an Aerobic: Anaerobic Sequencing Batch Reactor (SBR) with no Phosphorus Removal

Cited by 49 publications

References 38 publications

Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic–aerobic activated sludge processes

Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic–aerobic activated sludge processes

Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant

From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor

Contact Info

Product

Resources

About