Structure and functional impact of seed region variant in MIR-499 gene family in bronchial asthma

Toraih, Eman A.; Hussein, Mohammad; Ageeli, Essam Al; Riad, Eman; AbdAllah, Nouran B.; Helal, Ghada M.; Fawzy, Manal S.

doi:10.1186/s12931-017-0648-0

Cited by 19 publications

(28 citation statements)

References 54 publications

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“…These results indicated that placental exosomes in early pregnancy may play a significant role in the maternal immune inflammatory process. Consistent with this hypothesis, miR-499 is reportedly involved in the immune inflammatory process 25 , suggesting that miR-499 may play a crucial role in maternal immune regulation during early pregnancy in dairy cows. To further investigate the differential abundance of placental exosomal bta-miR-499 in early pregnancy, we analyzed bta-miR-499 levels in exosomes isolated from the plasma of non-pregnant (G.D.0, n = 5) and early pregnant cows (G.D.60, n = 5).…”

Section: Resultsmentioning

confidence: 54%

Placental exosome-mediated Bta-miR-499-Lin28B/let-7 axis regulates inflammatory bias during early pregnancy

Zhao

Yang

et al. 2018

Cell Death Dis

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Abnormal inflammatory bias in the maternal-fetal interface leads to reproductive failure in mammals. Placental exosomes are involved in maternal-fetal communication during pregnancy. However, whether the placenta or fetus is involved in regulating the balance of uterine local inflammation through exosomes remains unclear, and the mechanism must be further explored. Here we demonstrated that placenta-specific exosomes are abundant in the peripheral blood of dairy cows during early pregnancy and selectively load miRNAs, such as bta-miR-499. In vitro, placental exosome-derived bta-miR-499 inhibits the activation of NF-κB via the Lin28B/let-7 axis, thus repressing LPS-induced inflammation in bovine endometrial epithelial (BEND) cells. Subsequently, inhibition of mmu-miR-499 leads to an impaired balance of inflammation at the maternal-fetal interface in vivo, resulting in an increased risk of pregnancy failure due to placental loss and fetal growth restriction. Thus, our data demonstrate that placental exosomal miR-499 may be a critical immune regulator in the regulation of the inflammation balance at the maternal-fetal interface in the early gestation of dairy cows and other mammals.

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Section: Resultsmentioning

confidence: 54%

Placental exosome-mediated Bta-miR-499-Lin28B/let-7 axis regulates inflammatory bias during early pregnancy

Zhao

Yang

et al. 2018

Cell Death Dis

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“…MiR-499 is also associated significantly with myocardial infarction, and together with miR-133a and miR-208a, they represent potential early diagnostic biomarkers of myocardial infarction [97][98][99][100]. Furthermore, miR-499 is significantly involved in the inflammatory signaling pathways of bronchial asthma [101]. No data on the role of miR-499a-5p in pathogenesis of endothelial dysfunction are currently available.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

Hromadníková

Dvořáková

Krofta

2020

IJMS

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The aim of the study was to examine the effect of previous pregnancies and classical cardiovascular risk factors on vascular endothelial function in a group of 264 young and middle-aged women 3 to 11 years postpartum. We examined microvascular functions by peripheral arterial tonometry and EndoPAT 2000 device with respect to a history of gestational hypertension, preeclampsia, fetal growth restriction, the severity of the disease with regard to the degree of clinical signs and delivery date. Besides, we compared Reactive Hyperemia Index (RHI) values and the prevalence of vascular endothelial dysfunction among the groups of women with normal and abnormal values of BMI, waist circumference, systolic and diastolic blood pressures, heart rate, total serum cholesterol levels, serum high-density lipoprotein cholesterol levels, serum low-density lipoprotein cholesterol levels, serum triglycerides levels, serum lipoprotein A levels, serum C-reactive protein levels, serum uric acid levels, and plasma homocysteine levels. Furthermore, we determined the effect of total number of pregnancies and total parity per woman, infertility and blood pressure treatment, presence of trombophilic gene mutations, current smoking of cigarettes, and current hormonal contraceptive use on the vascular endothelial function. We also examined the association between the vascular endothelial function and postpartum whole peripheral blood expression of microRNAs involved in pathogenesis of cardiovascular/cerebrovascular diseases (miR-1-3p, miR-16-5p, miR-17-5p, miR-20a-5p, miR-20b-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-29a-3p, miR-92a-3p, miR-100-5p, miR-103a-3p, miR-125b-5p, miR-126-3p, miR-130b-3p, miR-133a-3p, miR-143-3p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-181a-5p, miR-195-5p, miR-199a-5p, miR-210-3p, miR-221-3p, miR-342-3p, miR-499a-5p, and miR-574-3p). A proportion of overweight women (17.94% and 20.59%) and women with central obesity (18.64% and 21.19%) had significantly lower RHI values at 10.0% false positive rate (FPR) both before and after adjustment of the data for the age of patients. At 10.0% FPR, a proportion of women with vascular endothelial dysfunction (RHI ≤ 1.67) was identified to have up-regulated expression profile of miR-1-3p (11.76%), miR-23a-3p (17.65%), and miR-499a-5p (18.82%) in whole peripheral blood. RHI values also negatively correlated with expression of miR-1-3p, miR-23a-3p, and miR-499a-5p in whole peripheral blood. Otherwise, no significant impact of other studied factors on vascular endothelial function was found. We suppose that screening of these particular microRNAs associated with vascular endothelial dysfunction may help to stratify a highly risky group of young and middle-aged women that would benefit from early implementation of primary prevention strategies. Nevertheless, it is obvious, that vascular endothelial dysfunction is just one out of multiple cardiovascular risk factors which has only a partial impact on abnormal expression of cardiovascular and cerebrovascular disease associated microRNAs in whole peripheral blood of young and middle-aged women.

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“…Another candidate miRNA in atopic asthma is miR-499. Toraih et al [ 50 ] demonstrated that a polymorphism (rs3746444), resulting in A>G substitution in the seed region of this miRNA gene, was associated with asthma risk in the population of Egyptian children and adults. The same polymorphism showed a significant association with spirometry results (increased forced expiratory volume in 1 s, FEV 1 ) in a population of Korean patients with AG/GG genotype in comparison to patients with AA genotype [ 51 ].…”

Section: Ncrna Studies In Asthmamentioning

confidence: 99%

Non-Coding RNAs in Pediatric Airway Diseases

Narożna

Langwiński

Szczepankiewicz

2017

Genes

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Non-coding RNAs (ncRNAs) are involved in the regulation of numerous biological processes and pathways and therefore have been extensively studied in human diseases. Previous reports have shown that non-coding RNAs play a crucial role in the pathogenesis and aberrant regulation of respiratory diseases. The altered expression of microRNAs (miRNAs) and long non-coding RNAs in blood and also locally in sputum or exhaled breath condensate influences lung function, immune response, and disease phenotype and may be used for the development of biomarkers specific for airway disease. In this review, we provide an overview of the recent works studying the non-coding RNAs in airway diseases, with a particular focus on chronic respiratory diseases of childhood. We have chosen the most common chronic respiratory condition—asthma—and the most severe, chronic disease of the airways—cystic fibrosis. Study of the altered expression of non-coding RNAs in these diseases may be key to better understanding their pathogenesis and improving diagnosis, while also holding promise for the development of therapeutic strategies using the regulatory potential of non-coding RNAs.

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Structure and functional impact of seed region variant in MIR-499 gene family in bronchial asthma

Cited by 19 publications

References 54 publications

Placental exosome-mediated Bta-miR-499-Lin28B/let-7 axis regulates inflammatory bias during early pregnancy

Placental exosome-mediated Bta-miR-499-Lin28B/let-7 axis regulates inflammatory bias during early pregnancy

Evaluation of Vascular Endothelial Function in Young and Middle-Aged Women with Respect to a History of Pregnancy, Pregnancy-Related Complications, Classical Cardiovascular Risk Factors, and Epigenetics

Non-Coding RNAs in Pediatric Airway Diseases

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