Structure and intracellular antioxidant activity of pectic polysaccharide from acerola (Malpighia emarginata)

Klosterhoff, Rafael; Bark, Juliana Müller; Glänzel, Nícolas Manzke; Iacomini, Marcello; Martinez, Gláucia Regina; Winnischofer, Sheila Maria Brochado; Cordeiro, Lucimara M.C.

doi:10.1016/j.ijbiomac.2017.08.032

Cited by 49 publications

(13 citation statements)

References 47 publications

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“…When cells are in the state of oxidative stress, they cannot scavenge the free radicals generated by mitochondria. As previous research reported [43][44][45][46][47], the antioxidant activity of polysaccharides is associated with their composition of sugar chains, branched chains, molecular weight, substituents, and conformation. The higher the molecular weight and the higher the content of uronic acid the polysaccharide has, the stronger its antioxidant ability.…”

Section: Discussionmentioning

confidence: 89%

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A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities

Lin

Liu

et al. 2020

Antioxidants

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After extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1,4-linked GalA (93.48%) with side chains of 1,2,4-linked Rha and 1,3,5-linked Ara and terminals of 1-linked Rha and 1-linked Ara, which might be the homogalacturonan (HG) type with side chains of the RG-I type, corresponding to the results of NMR. In AFM and SEM images, self-assembly and aggregation of SAZMP4 were respectively observed indicating its structural features. The antioxidant activity of SAZMP4 against H 2 O 2 -induced oxidative stress in Caco-2 cells was determined by activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as malondialdehyde (MDA) and reactive oxygen species (ROS) levels, indicating SAZMP4 can be a natural antioxidant. Also, a better water retention capacity and thermal stability of SAZMP4 was observed based on DSC analysis, which could be applied in food industry as an additive.

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Section: Discussionmentioning

confidence: 89%

“…As polysaccharides can only be digested in the intestinal tract, this study used Caco-2 as the cell model and H 2 O 2 as the irritant to induce cellular oxidative stress [47]. The antioxidant system in the human body has the ability to recover and regulate itself.…”

Section: Discussionmentioning

confidence: 99%

A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities

Lin

Liu

et al. 2020

Antioxidants

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“…Com a equação y = 11,489x + 0,0153 (R 2 de 0,9988) foi possível calcular a quantidade de amostra necessária para capturar (neutralizar) 50 % dos radicais presentes em solução (CI 50 O valor da atividade antioxidante apresentou-se excelente ao comparar com a atividade antioxidante da Malpighia emarginata encontrada por Rezende, Nogueira e Narain ( 2017), (CI 50 de 10 g.mL -1 ), Rufino et al (2010) (CI 50 de 670 e 49,2 g.g -1 ), SOUSA (2010) (CI 50 de 308,07 mg.mL -1 e CI 50 de 386,46 ± 1,41 mg.mL -1 ), Pereira et al (2013) (CI 50 de 359,42 µg.mL -1 , 418,48 µg.mL -1 e 471,42 µg.mL -1 ) e Klosterhoff et al (2018) (IC 100 µg/mL -1 ).…”

Section: Atividade Antioxidanteunclassified

Atividade antioxidante e antimicrobiana de extrato de Malpighia emarginata e sua aplicação em revestimentos à base de gelatina

Rampanelli

Rossi

Dinnebier

et al. 2020

PERSP

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O objetivo do estudo foi investigar as atividades antioxidante e antimicrobiana do extrato de Malpighia emarginata a partir dos frutos e sua aplicação em revestimento à base de gelatina na conservação de maçã minimamente processada. A atividade antioxidante e a atividade antimicrobiana foram realizadas para o extrato de Malpighia emarginata. Diferentes tratamentos (sem adição do revestimento, com revestimento e revestimento com extrato de Malpighia emarginata) foram aplicados como métodos de conservação das maçãs minimamente processadas. O extrato de Malpighia emarginata apresenta atividade antioxidante de 4,34 µg.mL-1 e não apresentou atividade antimicrobiana. A aplicação do extrato ao revestimento à base de gelatina não aumentou a vida de prateleira em relação à cor dos cortes de maçã.

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“…Molecular weight, homogeneity, total uronic acid, and monosaccharide composition analyses were carried out [73,74]. The study included mainly a pectic polysaccharide and biological model [75]. The research showed the capacity of pectin to protect the cell against the cytotoxicity that was generated by H 2 O 2 by means of a decrease of intracellular reactive oxygen species levels.…”

Section: Outline Of Biological Activities Of Pectic Polysaccharidesmentioning

confidence: 99%

Biological Activity and Pharmacological Application of Pectic Polysaccharides: A Review

et al. 2018

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Pectin is a polymer with a core of alternating α-1,4-linked d-galacturonic acid and α-1,2-l-rhamnose units, as well as a variety of neutral sugars such as arabinose, galactose, and lesser amounts of other sugars. Currently, native pectins have been compared to modified ones due to the development of natural medicines and health products. In this review, the results of a study of the bioactivity of pectic polysaccharides, including its various pharmacological applications, such as its immunoregulatory, anti-inflammatory, hypoglycemic, antibacterial, antioxidant and antitumor activities, have been summarized. The potential of pectins to contribute to the enhancement of drug delivery systems has been observed.

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Structure and intracellular antioxidant activity of pectic polysaccharide from acerola (Malpighia emarginata)

Cited by 49 publications

References 47 publications

A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities

A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities

Atividade antioxidante e antimicrobiana de extrato de Malpighia emarginata e sua aplicação em revestimentos à base de gelatina

Biological Activity and Pharmacological Application of Pectic Polysaccharides: A Review

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