Structure and sequence of a wheat phosphoribulokinase gene

Lloyd, Julie C.; Horsnell, Philip R.; Dyer, Tristan A.; Raines, Christine A.

doi:10.1007/bf00036823

Cited by 7 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We are interested in the mechanisms which control the expression of Calvin cycle genes and lead to the establishment of a functional cycle during leaf development. In working towards this objective we have isolated genes encoding a number of Calvin cycle enzymes from wheat [ 19,20,30] and Arabidopsis thaliana (unpublished data). This paper describes the isolation and nucleotide sequence analysis of the A. thaliana SBPase gene and compares its structure with the wheat SBPase gene.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-biphosphatase gene and expression studies in wheat and Arabidopsis thaliana

1994

Self Cite

View full text Add to dashboard Cite

We report here the isolation and nucleotide sequence of genomic clones encoding the chloroplast enzyme sedoheptulose-1,7-bisphosphatase (SBPase) from Arabidopsis thaliana. The coding region of this gene contains eight exons (72-76 bp) and seven introns (75-91 bp) and encodes a polypeptide of 393 amino acids. Unusually, the 5' non-coding region contains two additional AUG codons upstream of the translation initiation codon. A comparison of the deduced Arabidopsis and wheat SBPase polypeptide sequences reveals 78.6%, identity. Expression studies showed that the level of SBPase mRNA in Arabidopsis and wheat is regulated in a light-dependent manner and is also influenced by the developmental stage of the leaf. Although the Arabidopsis SBPase gene is present in a single copy, two hybridizing transcripts were detected in some tissues, suggesting the presence of alternate transcription start sites in the upstream region.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-biphosphatase gene and expression studies in wheat and Arabidopsis thaliana

1994

Self Cite

View full text Add to dashboard Cite

show abstract

“…sphaeroides (Gibson et al, 1990;Gibson et al, 1991), Alcaligenes eutrophus (Kossmann et al, 1989), and Xanthobacter flavus (Meijer et al, 1990), indicates very close homology. Interestingly, however, only slight sequence homology of bacterial PRKs towards the plant (Milanez and Mural, 1988;Lloyd et al, 1991), algal (Roesler and Ogren, 1990), or cyanobacterial (Su and Bogorad, 1991) enzymes is apparent; for example the plant enzymes share only 13% identity towards the Rb. sphaeroides proteins.…”

Section: B Phosphoribulokinase (Prk)mentioning

confidence: 99%

The Biochemistry and Metabolic Regulation of Carbon Metabolism and CO2 Fixation in Purple Bacteria

Tabita

Advances in Photosynthesis and Respiration

101

134

View full text Add to dashboard Cite

“…Competitor fragment concentrations were determined spectrophotometrically. [13] and FBPase [14] genes showing sites used to generate probe fragments. Transcription start sites are indicated by arrows.…”

Section: Probes and Competitor Dna Preparationmentioning

confidence: 99%

“…Figure la shows the sequence included in the SBPase probe fragments. We have previously published the upstream nucleotide sequences of the wheat FBPase [14] and PRKase [13] genes. A schematic representation of all of these sequences showing the restriction enzyme sites used to generate the probe fragments is shown in Fig. lb.…”

Section: Calvin Cycle Gene Probesmentioning

confidence: 99%

A light- and developmentally-regulated DNA-binding interaction is common to the upstream sequences of the wheat Calvin cycle bisphosphatase genes

et al. 1993

Self Cite

View full text Add to dashboard Cite

We have characterised a DNA-binding interaction common to the upstream sequences of the wheat fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) genes. The recognition site for this sequence-specific binding activity, designated wheat FBPase factor (WF-1), is located within 125 bp of the transcription start site of each gene. Within these regions there are no sequence motifs similar to those shown to be important for light-regulated expression in other species. The binding activity was not detected in wheat root nuclear extracts, or in pea leaf extracts. There was a higher level of binding activity in light-grown than in dark-grown wheat leaves. The level was also found to decline when light-grown plants were given an extended dark treatment, but could be reinduced by light. Utilising the gradient of developmental maturity which exists within the wheat leaf it was found that WF-1 activity increases during leaf development.

show abstract

Structure and sequence of a wheat phosphoribulokinase gene

Cited by 7 publications

References 2 publications

Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-biphosphatase gene and expression studies in wheat and Arabidopsis thaliana

Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-biphosphatase gene and expression studies in wheat and Arabidopsis thaliana

The Biochemistry and Metabolic Regulation of Carbon Metabolism and CO2 Fixation in Purple Bacteria

A light- and developmentally-regulated DNA-binding interaction is common to the upstream sequences of the wheat Calvin cycle bisphosphatase genes

Contact Info

Product

Resources

About