Structure Characterization and Action Mechanism of an Antiaging New Compound from <i>Gastrodia elata</i> Blume

Farooq, Umer; Pan, Yanjun; Yang, Lin; Wang, Ying; Osada, Hiroyuki; Xiang, Lan; Qi, Jianhua

doi:10.1155/2019/5459862

Cited by 17 publications

(25 citation statements)

References 37 publications

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“…Aged yeast showed a significantly lower expression of the SIR2, SOD1 and SOD2 genes compared to young yeast, whereas, as already observed, the positive control RES could reverse that trend [28][29][30][31]. The expression of the SIR2, SOD1 and SOD2 genes was also significantly increased by AE and CHL.…”

Section: Almond Skin Extract and Chlorogenic Acid Activated Expressiosupporting

confidence: 70%

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Almond Skin Extracts and Chlorogenic Acid Delay Replicative Aging by Enhanced Oxidative Stress Response Involving SIR2 and SOD2 in Yeast

Abid¹,

Elamrani²,

Drouet³

et al. 2020

Preprint

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Almond (Prunus dulcis (Mill.) D.A.Webb) is one of the largest nut crops in the world. Recently, phenolic compounds, mostly stored in almond skin, have been associated with much of the health-promoting behavior associated with their intake. The almond skin enriched fraction obtained from cold-pressed oil residues of the endemic Moroccan Beldi ecotypes is particularly rich in chlorogenic acid. In this study, both almond skin extract (AE) and chlorogenic acid (CHL) supplements, similar to traditional positive control resveratrol, significantly increased the replicative life-span of yeast compared to the untreated group. Our results showed that AE and CHL significantly reduced the production of reactive oxygen and nitrogen species (ROS/RNS), most likely due to their ability to maintain mitochondrial function during aging, as indicated by the maintenance of normal mitochondrial membrane potential in treated groups. This may be associated with the observed activation of the anti-oxidative stress response in treated yeast, which results in activation at both gene expression and enzymatic activity levels for SOD2 and SIR2, the latter being an upstream inducer of SOD2 expression. Interestingly, the differential gene expression induction of mitochondrial SOD2 gene at the expense of the cytosolic SOD1 gene confirms the key role of mitochondrial function in this regulation. Furthermore, AE and CHL have contributed to the survival of yeast under UV-C-induced oxidative stress, by reducing the development of ROS / RNS, resulting in a significant reduction in cellular oxidative damage as evidenced by decreased membrane lipid peroxidation, protein carbonyl content and 8-oxo-guanine formation in DNA. Together, these results demonstrate the interest of AE and CHL as new regulators in the replicative life-span and control of the oxidative stress response of yeast.

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Section: Almond Skin Extract and Chlorogenic Acid Activated Expressiosupporting

confidence: 70%

“…Control cells of yeast have been inoculated with the same volume of DMSO. Resveratrol (RES) was used as a positive control at a classically applied concentration of 10 μM[28][29][30][31].…”

mentioning

confidence: 99%

Almond Skin Extracts and Chlorogenic Acid Delay Replicative Aging by Enhanced Oxidative Stress Response Involving SIR2 and SOD2 in Yeast

Abid¹,

Elamrani²,

Drouet³

et al. 2020

Preprint

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“…Y4741 yeast in the liquid glucose medium was treated with RES (10 μ M) or amarogentin (0, 1, 3, and 10 μ M) for 48 h at 28°C. The activity assays of total SOD, SOD1, SOD2, GPx, and CAT were performed according to a previous study [ 23 , 24 ]. The BY4741 yeast cells in the liquid glucose medium was treated with RES (10 μ M) or amarogentin (0, 1, 3, and 10 μ M) for 24 h at 28°C with the initial OD of 0.1.…”

Section: Methodsmentioning

confidence: 99%

“…Natural products are important sources of drugs to reduce or prevent oxidative stresses in humans. In our previous studies, 19 molecules were isolated from natural products which were able to extend the replicative lifespan of yeast via antioxidative stress [20][21][22][23][24][25]. Therefore, searching for effective molecules with antioxidative stress potential should be considered an important strategy to treat aging and neurodegenerative disorders.…”

Section: Introductionmentioning

confidence: 99%

“…A unique characteristic of K6001 is that only mother cells can produce daughter cells in a glucose medium but not in a galactose medium [26]. In our previous study, K6001, which is a yeast mutant strain, was used as a bioassay system to evaluate the antiaging activity of natural products [20][21][22][23][24][25]. Furthermore, the PC12 cell line, which was derived from rat pheochromocytoma cells, is one of the useful models for studying the nervous system at the cellular level [27].…”

Section: Introductionmentioning

confidence: 99%

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Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress

Disasa

Cheng

Manzoor

et al. 2020

Oxidative Medicine and Cellular Longevity

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In the present study, the replicative lifespan assay of yeast was used to guide the isolation of antiaging substance from Gentiana rigescens Franch, a traditional Chinese medicine. A compound with antiaging effect was isolated, and the chemical structure of this molecule as amarogentin was identified by spectral analysis and compared with the reported data. It significantly extended the replicative lifespan of K6001 yeast at doses of 1, 3, and 10 μM. Furthermore, amarogentin improved the survival rate of yeast under oxidative stress by increasing the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and these enzymes’ gene expression. In addition, this compound did not extend the replicative lifespan of sod1, sod2, uth1, and skn7 mutants with K6001 background. These results suggested that amarogentin exhibited antiaging effect on yeast via increase of SOD2, CAT, GPx gene expression, enzyme activity, and antioxidative stress. Moreover, we evaluated antioxidant activity of this natural products using PC12 cell system, a useful model for studying the nervous system at the cellular level. Amarogentin significantly improved the survival rate of PC12 cells under H2O2-induced oxidative stress and increased the activities of SOD and SOD2, and gene expression of SOD2, CAT, GPx, Nrf2, and Bcl-x1. Meanwhile, the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) of PC12 cells were significantly reduced after treatment of the amarogentin. These results indicated that antioxidative stress play an important role for antiaging and neuroprotection of amarogentin. Interestingly, amarogentin exhibited neuritogenic activity in PC12 cells. Therefore, the natural products, amarogentin from G. rigescens with antioxidant activity could be a good candidate molecule to develop drug for treating neurodegenerative diseases.

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Bioprospection of Orchids and Appraisal of Their Therapeutic Indications

Ghai

Verma

Kaur

et al. 2021

Bioprospecting of Plant Biodiversity for Industrial Molecules

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Structure Characterization and Action Mechanism of an Antiaging New Compound from Gastrodia elata Blume

Cited by 17 publications

References 37 publications

Almond Skin Extracts and Chlorogenic Acid Delay Replicative Aging by Enhanced Oxidative Stress Response Involving SIR2 and SOD2 in Yeast

Almond Skin Extracts and Chlorogenic Acid Delay Replicative Aging by Enhanced Oxidative Stress Response Involving SIR2 and SOD2 in Yeast

Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress

Bioprospection of Orchids and Appraisal of Their Therapeutic Indications

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