Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum

Yue, Heng; Xu, Qianqian; Bian, Guoyong; Guo, Qing; Fang, Zhou; Wu, Wenhui

doi:10.1016/j.ijbiomac.2019.11.071

Cited by 20 publications

(10 citation statements)

References 40 publications

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“…The signal at 4.48 and 3.96 ppm belonged to the H-1 and H-6 of 1, 6-β-D-Gal linkage [ 18 , 30 ]. The signal at 4.23 ppm was assigned to the H-4 of 1, 4-β-D-Gal linkage, and the peaks at 73.18 and 78.93 ppm were connected to the C-2 and C-4 of 1, 4-β-D-Gal linkage [ 18 , 31 ]. The peaks at 5.11 and 4.02 ppm suggested the H-1 and H-3 of 1, 5-α-L-Ara linkage, respectively, with signals at 109.16 and 83.79 ppm were derived from the C-1 and C-2 of 1, 5-α-L-Ara linkage [ 32 ], respectively.…”

Section: Resultsmentioning

confidence: 99%

Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques

et al. 2021

Molecules

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In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated. Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies. Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively. The high contents of bound polyphenolics were measured in LLP-H and LLP-M, as well as polysaccharides obtained from lotus leaves by vacuum drying (LLP-V). Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures. Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase. Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others. These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.

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Section: Resultsmentioning

confidence: 99%

Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques

et al. 2021

Molecules

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“…Previously, many studies have reported that neutral polysaccharides have potential as an immunomodulator or supplement in functional food to enhance immunity. For example, a neutral polysaccharide SMP-0b extracted from Solanum muricatum could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells (Yue et al, 2020); a neutral polysaccharide SPW-2, purified from the leaves of Sambucus adnata Wall, exerted an immunomodulatory effect by activating macrophages and enhancing the host immune system function (Yuan et al, 2020); a neutral polysaccharide WSRP-1b from Kushui rose waste possessed immunomodulatory activity by enhancing phagocytosis of macrophages, increasing the production of ROS, NO, cytokines (IL-6 and TNF-α), and activating the NF-κB signaling pathway (Wu et al, 2020). Inspired by these studies of the immunomodulatory activity of neutral polysaccharides, we also examined the immunomodulatory ability of LHPW through a series of experiments.…”

Section: Discussionmentioning

confidence: 99%

Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide From Lycopi Herba

Zhang

et al. 2021

Front. Pharmacol.

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Lycopi Herba has been broadly used as a traditional medicinal herb in Asia due to its ability to strengthen immunity. However, it is still obscure for its material basis and underlying mechanisms. Polysaccharide, as one of the most important components of most natural herbs, usually contributes to the immunomodulatory ability of herbs. Here, we aimed to detect polysaccharides from Lycopi Herba and examine their potential immunomodulatory activity. A novel polysaccharide (LHPW) was extracted from Lycopi Herba and purified by DEAE-52 cellulose chromatography and G-100 sephadex. According to physicochemical methods and monosaccharide composition analysis, LHPW was mainly composed of galactose, glucose, fructose, and arabinose. NMR and methylation analyses indicated that LHPW was a neutral polysaccharide with a backbone containing →3,6)-β-D-Galp-(1→, →4)-β-D-Galp-(1→ and →4)-α-D-Glcp-(1→, with the branches of →1)-β-D-Fruf-(2→ and →6)-α-D-Galp-(1→. Immunological tests indicated that LHPW could activate macrophage RAW264.7 and promote splenocyte proliferation. This study discovered a novel polysaccharide from Lycopi Herba and showed it was a potential immunomodulator.

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“…The weak signal at 2.16 ppm was tentatively deduced to be the existence of acetyl groups [29], and the strong peak at 3.81 ppm was tentatively deduced to be the signal of methyl esters connecting to carboxyl groups of D-GalA [29]. In addition, the peak at 4.46 ppm was tentatively deduced to be the H-1 of 1,6-β-D-Gal, and the peaks at 4.23 and 3.72 ppm were tentatively deduced to be the H-4 and H-5 of 1,4-β-D-Gal [29,30], respectively. The peak at 3.95 ppm was tentatively deduced to be the H-3 of 1,4-α-D-Glc [29].…”

Section: Nmr Analysismentioning

confidence: 95%

The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies

Guo

Zhao

et al. 2021

Foods

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Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared. Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies. Protein-bound polysaccharides and some hemicellulose could be extracted from sweet tea with diluted alkali solution. STPs extracted by deep-eutectic solvents and diluted alkali solution exhibited the most favorable biological properties. Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties. To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.

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Structure characterization and immunomodulatory activity of a new neutral polysaccharide SMP-0b from Solanum muricatum

Cited by 20 publications

References 40 publications

Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques

Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques

Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide From Lycopi Herba

The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies

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