Structure characterization of soybean peptides and their protective activity against intestinal inflammation

Peptides and protein hydrolysates exhibiting anti-inflammatory activity: sources, structural features and modulation mechanisms

“…Specifically, peptides YGGGGE and SEGGFLE obtained by hydrolysis with a mixture of Alcalase/Neutrase reported protective activity against intestinal inflammation, as well as peptide SLVNNDDRDS, named S-10-S, obtained by trypsin hydrolysis, that exerted its effect through inhibition of the NF-κB pathway and downregulation of the gene expression of IL-1β and TNF-α. 61,86…”

“…25,60 Finally, the bioactivity of purified peptides is validated in vitro and/or in vivo through the quantification of inflammatory mediators such as prostaglandin E2 (PGE2), TNF-α, IL-6, IL-1β, IL-10, IL-8, VCAM-1 or NO in cell lines or by measuring the inhibition of enzymes involved in inflammation such as COX-2, LOX and iNOS. [61][62][63][64] Nonetheless, current techniques for separation, purification, identification, and quantification of biopeptides from their natural matrices are limited by high technicality and high cost, which restricts their therapeutic competitiveness and industrial application. 53 Processes for peptide preparation and identification represent an analytical challenge because they implie high yield and purity for the quantification of its potential bioactivity and the validation of their structure-activity relationship.…”

“…Specifically, peptides YGGGGE and SEGGFLE obtained by hydrolysis with a mixture of Alcalase/Neutrase reported protective activity against intestinal inflammation, as well as peptide SLVNNDDRDS, named S-10-S, obtained by trypsin hydrolysis, that exerted its effect through inhibition of the NF-κB pathway and downregulation of the gene expression of IL-1β and TNFα. 61,86 The enzymes Corolase PP and Alcalase have also been used successfully to hydrolyze leguminosae plant proteins and identify anti-inflammatory peptides such as GPETAFLR an octapeptide derived from lupine protein hydrolyzed with alcalase that exerted protection against inflammatory damage in retinal pigment epithelium cells, as well as modulated the inflammatory response and plasticity in human primary monocytes. 121,122 Moreover, GPETAFLR inhibited neuroinflammation by preventing inflammation in BV-2 microglial cells and potentiating neuroprotection in mouse brain.…”

Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway

“…Globulins were studied as one of the major proteins from soy, like in amaranth, increasing the weight of evidence to confirm that kiwicha peptides could have an effect in in vivo models to manage intestinal inflammation. In fact, the recent structural characterization of soybean peptides and their protective activity against intestinal inflammation (which have been proven to reduce the production and expression of IL-1β and IL-6) were addressed by Wen et al , 33 who showed the beneficial effects of these peptides on gut health. A review of the biological functions of peptides from legumes in gastrointestinal health can be found elsewhere.…”

Biological functions of peptides from legumes in gastrointestinal health. A review legume peptides with gastrointestinal protection

“…Wen, Bi, et al (2022), observed that soybean peptides prepared by Alcalase® and neutrase, alleviate LPS‐induced inflammation by reducing the production and expression of NO, TNFα, IL‐1β, IL‐6 and promoting the mRNA expression of proteins related to inflammation inhibition (IL‐10).…”