Structure deformation and curvature sensing of PIEZO1 in lipid membranes

Yang, Xu-Zhong; Lin, Chao; Chen, Xudong; Li, Shouqin; Li, Xueming; Xiao, Bailong

doi:10.1038/s41586-022-04574-8

Cited by 133 publications

(177 citation statements)

References 52 publications

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“…Moreover, since Piezo1 activity is affected by the lipidic composition of the cell membrane [ 39 ], a correct cell surface flip-flop of phosphatidylserine was found to control the myoblast fusion by regulating Piezo1 activity via a force from lipids effect [ 26 ]. In line with this, the increased exposure of phosphatidylserine induced by TMEM16F scramblase stimulates the fusion of the C2C12 cells [ 40 ], whereas the prevalence of phosphatidylserine inner translocation mediated by P4-ATP-ase flippase avoids the excess of syncytia formation [ 26 ].…”

Section: The Role Of Piezo1 During Adult Myogenesismentioning

confidence: 99%

“…Due to the potential implication of Piezo1 in cardiovascular and inflammatory conditions [ 43 , 46 , 77 ], increasing attention has been directed in the last years to characterize new potential treatments capable of pharmacologically modulating Piezo1 function, or acting on the elastic properties of the plasma membrane, such as its lipid/cholesterol components, which regulate Piezo1 function in a dynamic manner [ 39 ]. Some in vitro studies underline statins, methyl-β-cyclodextrin, or dynasore (a GTPase inhibitor) and dietary fatty acid supplementation, in modulating Piezo 1 activation/inhibition by acting on the membrane cholesterol content and fatty acid metabolism [ 33 , 34 , 78 , 79 ].…”

Section: Translational Perspectives For Developing Piezo1-based Thera...mentioning

confidence: 99%

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The State of the Art of Piezo1 Channels in Skeletal Muscle Regeneration

Bernareggi

Bosutti

Massaria

et al. 2022

IJMS

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Piezo1 channels are highly mechanically-activated cation channels that can sense and transduce the mechanical stimuli into physiological signals in different tissues including skeletal muscle. In this focused review, we summarize the emerging evidence of Piezo1 channel-mediated effects in the physiology of skeletal muscle, with a particular focus on the role of Piezo1 in controlling myogenic precursor activity and skeletal muscle regeneration and vascularization. The disclosed effects reported by pharmacological activation of Piezo1 channels with the selective agonist Yoda1 indicate a potential impact of Piezo1 channel activity in skeletal muscle regeneration, which is disrupted in various muscular pathological states. All findings reported so far agree with the idea that Piezo1 channels represent a novel, powerful molecular target to develop new therapeutic strategies for preventing or ameliorating skeletal muscle disorders characterized by an impairment of tissue regenerative potential.

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Section: The Role Of Piezo1 During Adult Myogenesismentioning

confidence: 99%

Section: Translational Perspectives For Developing Piezo1-based Thera...mentioning

confidence: 99%

The State of the Art of Piezo1 Channels in Skeletal Muscle Regeneration

Bernareggi

Bosutti

Massaria

et al. 2022

IJMS

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“…They use this radius of curvature to compare the predictions of the membrane dome model (6) to Piezo gating in a patch of membrane (16), which is not a 10 nm vesicle. The authors of (10) might have noticed in Figure 1 of their paper that in larger vesicles, Piezo's radius of curvature is greater than 10 nm, as was also shown previously (9), and they ought to have wondered what it would be in a planar membrane. As we have shown, it is closer to 40 nm.…”

Section: Discussionmentioning

confidence: 75%

Elastic properties and shape of the Piezo dome underlying its mechanosensory function

Haselwandter

Guo

et al. 2022

Preprint

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In a companion paper we show that the free membrane shape of lipid bilayer vesicles containing the mechanosensitive ion channel Piezo can be predicted, with no free parameters, from membrane elasticity theory together with measurements of the protein geometry and vesicle size (accompanying paper). Here we use these results to determine the force that Piezo exerts on the free membrane and, hence, that the free membrane exerts on Piezo, for a range of vesicle sizes. From vesicle shape measurements alone, we thus obtain a force-distortion relationship for Piezo, from which we deduce Piezo's intrinsic radius of curvature, 42 +- 12 nm, and bending stiffness, 18 +- 2.1k!T, in free-standing lipid bilayer membranes mimicking cell membranes. Applying these estimates to a spherical cap model of Piezo embedded in a lipid bilayer, we suggest that Piezo's intrinsic curvature, surrounding membrane footprint, small stiffness, and large area are the key properties of Piezo that give rise to low-threshold, high-sensitivity mechanical gating.

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“…MD became the method of choice for unraveling nanoscale phenomena since it yields great accuracy in understanding the properties of lipid membranes and lipid−protein interactions that can describe biological membranes in a relatively realistic manner [ 18 ]. Nevertheless, although computational models have provided important insight into the organization of plasma membrane and protein-lipid interactions, and interesting and relevant work has been performed on artificial membranes [ 19 , 20 , 21 ], they do not necessarily give an entirely accurate insight into protein-lipid interactions as they happen in membranes of living organisms.…”

Section: Membrane Dynamics and Lipid-protein Interplaymentioning

confidence: 99%

Start Me Up: How Can Surrounding Gangliosides Affect Sodium-Potassium ATPase Activity and Steer towards Pathological Ion Imbalance in Neurons?

et al. 2022

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Gangliosides, amphiphilic glycosphingolipids, tend to associate laterally with other membrane constituents and undergo extensive interactions with membrane proteins in cis or trans configurations. Studies of human diseases resulting from mutations in the ganglioside biosynthesis pathway and research on transgenic mice with the same mutations implicate gangliosides in the pathogenesis of epilepsy. Gangliosides are reported to affect the activity of the Na+/K+-ATPase, the ubiquitously expressed plasma membrane pump responsible for the stabilization of the resting membrane potential by hyperpolarization, firing up the action potential and ion homeostasis. Impaired Na+/K+-ATPase activity has also been hypothesized to cause seizures by several mechanisms. In this review we present different epileptic phenotypes that are caused by impaired activity of Na+/K+-ATPase or changed membrane ganglioside composition. We further discuss how gangliosides may influence Na+/K+-ATPase activity by acting as lipid sorting machinery providing the optimal stage for Na+/K+-ATPase function. By establishing a distinct lipid environment, together with other membrane lipids, gangliosides possibly modulate Na+/K+-ATPase activity and aid in “starting up” and “turning off” this vital pump. Therefore, structural changes of neuronal membranes caused by altered ganglioside composition can be a contributing factor leading to aberrant Na+/K+-ATPase activity and ion imbalance priming neurons for pathological firing.

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Structure deformation and curvature sensing of PIEZO1 in lipid membranes

Cited by 133 publications

References 52 publications

The State of the Art of Piezo1 Channels in Skeletal Muscle Regeneration

The State of the Art of Piezo1 Channels in Skeletal Muscle Regeneration

Elastic properties and shape of the Piezo dome underlying its mechanosensory function

Start Me Up: How Can Surrounding Gangliosides Affect Sodium-Potassium ATPase Activity and Steer towards Pathological Ion Imbalance in Neurons?

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