Structure-dependent immune modulating activity of okra polysaccharide on THP-1 macrophages

Trakoolpolpruek, Thanathon; Moonmangmee, Somporn; Chanput, Wasaporn

doi:10.1016/j.bcdf.2018.10.002

Cited by 20 publications

(19 citation statements)

References 35 publications

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“…Furthermore, the TNF-α up-regulation was reported only after a 20 µg mL −1 okra polysaccharide treatment. 23 No substantial effect on the IL-1β and TNF-α gene expression was reported after treatment with digested okra polysaccharides. 23 Mairuae et al 24 stated that 50, 100, and 200 μg mL −1 okra extracts blocked the TNF-α and IL-1β production in LPS-stimulated BV2 microglia cells in a concentration-dependent manner.…”

Section: Reviewmentioning

confidence: 99%

“…23 No substantial effect on the IL-1β and TNF-α gene expression was reported after treatment with digested okra polysaccharides. 23 Mairuae et al 24 stated that 50, 100, and 200 μg mL −1 okra extracts blocked the TNF-α and IL-1β production in LPS-stimulated BV2 microglia cells in a concentration-dependent manner. Chen et al 25 reported that 25, 50, 100, and 200 μg mL −1 okra polysaccharides increased the TNF-α secretion in LPS-stimulated RAW264.7 macrophages in a dose-dependent manner.…”

Section: Reviewmentioning

confidence: 99%

“…Food & Function release and mRNA level. Trakoolpolpruek et al 23 reported that 10 and 20 µg mL −1 okra polysaccharides increased the IL-1β gene expression in THP-1 macrophages. Furthermore, the TNF-α up-regulation was reported only after a 20 µg mL −1 okra polysaccharide treatment.…”

Section: Reviewmentioning

confidence: 99%

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Effects of okra (Abelmoschus esculentusL) on inflammatory mediators: a systematic review of preclinical studies

2022

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

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Effects of okra (Abelmoschus esculentusL) on inflammatory mediators: a systematic review of preclinical studies

2022

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“…Vegetable mucilages have been studied due to their biological activities in humans and animals. The polysaccharides from these mucilages demonstrate some important biological activities, such as immunomodulated and anti-inflammatory [ 27 , 37 , 38 , 39 ].…”

Section: Beneficial Properties Of Okra Mucilage To Health and Its Relevance For Considering Okra As A Functional Foodmentioning

confidence: 99%

Okra (Abelmoschus esculentus L.) as a Potential Functional Food Source of Mucilage and Bioactive Compounds with Technological Applications and Health Benefits

Dantas

Buriti

Florentino

2021

Plants

100

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Abelmoschus esculentus has fruit popularly known as okra and belongs to the Malvaceae family. It is commonly used in cooking but also in traditional medicine in the treatment of worms, dysentery, inflammation, and also irritation of the stomach, intestines, and kidneys, as it is a potential functional food. Its mucilage is a highly viscous polysaccharide that is mostly composed of monosaccharides D-galactose, L-rhamnose, and galacturonic acid, as well as proteins and minerals. The functional properties of okra mucilage have been widely studied, mainly for its potential antidiabetic activity; thus, its use as adjuvant or nutraceutical therapy for diabetes is very promising. Due to its rheological properties, it is a potential resource for pharmaceutical and food applications. Okra mucilage can be extracted by several methods, which can directly influence its physicochemical characteristics and biological activity. Features such as low cost, non-toxicity, biocompatibility, and high availability in nature arouse the interest of researchers for the study of okra mucilage. The survey of research on the applications of okra mucilage highlights the importance of using this promising source of bioactive compounds with interesting technological properties. The potential of okra as a functional food, the properties of okra mucilage, and its technological applications are discussed in this review.

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“…Macrophages mainly exist in tissues or peripheral blood, and play a central role in host defense by removing pathogens and dead cells [ 18 , 19 ]. Therefore, the in vitro evaluation of macrophages activities after bioactive substance exposure is an important indicator to reflect the immunoregulatory capacity of compounds [ 20 ]. As reported, the molecular weights of polysaccharides would significantly affect the solubility and bioactivities, and low-molecular-weight polysaccharides could exhibit stronger activities due to the higher dissolubility and permeability compared with those of higher molecular weight [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Jiao

et al. 2022

Nutrients

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In recent years, multiple edible polysaccharides from Codonopsis pilosula were mainly isolated with high average molecular weights and exhibited various bioactivities, but it was proven that low-molecular-weight polysaccharides could exert stronger activities due to the superior water solubility and permeability. In the present study, the water-soluble polysaccharide C. pilosula with low molecular weight was isolated under ultrasonic assistance at 30 °C, the extraction process was optimized via response surface method (RSM), and the structure and immunoregulatory activity were further investigated. The maximum yield (4.86%) for crude polysaccharides (cCPPs) was obtained under following parameters: ultrasonic power of 370 W, liquid/material ratio of 33 mL/g, ultrasonic time of 81 min. Subsequently, the cCPPs were further purified through dialysis and Sephadex G-25 column to acquire purified polysaccharide (CPPs). Structural analysis indicated that CPPs was a glucofructan (average molecular weight of 4.23 × 103 Da) with (2→1)-β-D-Fruf and (1→)-α-D-Glcp as the backbone branched by (2→6)-β-D-Fruf. Additionally, CPPs could enhance immunoregulatory function by stimulating NO production and cytokine (IL-6 and TNF-α) secretion of RAW264.7 macrophages dose-dependently, which presented no cytotoxic effects. These data suggest that CPPs have the potential to be used as a nutritional dietary compound and natural immunostimulant supplement in the food industry.

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Structure-dependent immune modulating activity of okra polysaccharide on THP-1 macrophages

Cited by 20 publications

References 35 publications

Effects of okra (Abelmoschus esculentusL) on inflammatory mediators: a systematic review of preclinical studies

Effects of okra (Abelmoschus esculentusL) on inflammatory mediators: a systematic review of preclinical studies

Okra (Abelmoschus esculentus L.) as a Potential Functional Food Source of Mucilage and Bioactive Compounds with Technological Applications and Health Benefits

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

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